Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum)
The optimization of tissue culture protocols for blueberry propagation is crucial to meet the increasing demand for disease-free planting materials. This study aimed to determine the effects of three auxins (IBA, IAA, and NAA) at different concentrations to enhance root and shoot development of two...
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| Format: | Article |
| Language: | English |
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2024-12-01
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| Series: | Journal of Horticultural Research |
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| Online Access: | https://doi.org/10.2478/johr-2024-0016 |
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| author | Zalt Najla Yasmin Samina Hamakhan Awara M. Polat Şule Yu Hong Ge Chunfeng Imrak Burhanettin Kafkas Ebru |
| author_facet | Zalt Najla Yasmin Samina Hamakhan Awara M. Polat Şule Yu Hong Ge Chunfeng Imrak Burhanettin Kafkas Ebru |
| author_sort | Zalt Najla |
| collection | DOAJ |
| description | The optimization of tissue culture protocols for blueberry propagation is crucial to meet the increasing demand for disease-free planting materials. This study aimed to determine the effects of three auxins (IBA, IAA, and NAA) at different concentrations to enhance root and shoot development of two blueberry cultivars with different abilities to root, ‘Lanmei #1’and ‘Brightwell’. Results showed that NAA at 0.5 mg·L−1 significantly promoted shoot number and length in ‘Lanmei #1’ while ‘Brightwell’ showed a limited response. IAA had a variable impact on both shoot and root parameters, with ‘Lanmei #1’ showing more pronounced positive responses. IBA showed a differential response on root and shoot parameters in both cultivars, with ‘Lanmei #1’ showing a superior response for shoot number, length, root length, and overall plant vigor. Activated carbon had minimal effect on growth parameters across both cultivars. ‘Lanmei #1’ demonstrated a robust response to auxin treatments, suggesting it is more suitable for in vitro rooting. The present results confirm previous data on the different abilities of blueberry genotypes to root and the necessity of optimizing the rooting protocol depending on the cultivar. |
| format | Article |
| id | doaj-art-3a91889d3e5244bd8fd50462a969db4f |
| institution | DOAJ |
| issn | 2353-3978 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Horticultural Research |
| spelling | doaj-art-3a91889d3e5244bd8fd50462a969db4f2025-08-20T02:51:57ZengSciendoJournal of Horticultural Research2353-39782024-12-01322758010.2478/johr-2024-0016Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum)Zalt Najla0Yasmin Samina1Hamakhan Awara M.2Polat Şule3Yu Hong4Ge Chunfeng5Imrak Burhanettin6Kafkas Ebru71Department of Horticulture, Faculty of Agriculture, Çukurova University, Balcalı, Sarıçam, Adana 01330, Turkey1Department of Horticulture, Faculty of Agriculture, Çukurova University, Balcalı, Sarıçam, Adana 01330, Turkey2Bakrajo Technical Institute, Sulaimani Polytechnic University, Department of Plant Protection, Kurdistan Region, Iraq1Department of Horticulture, Faculty of Agriculture, Çukurova University, Balcalı, Sarıçam, Adana 01330, Turkey3Nanjing Botanical Garden, Memorial Sun Yat-Sen, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, China3Nanjing Botanical Garden, Memorial Sun Yat-Sen, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, China1Department of Horticulture, Faculty of Agriculture, Çukurova University, Balcalı, Sarıçam, Adana 01330, Turkey1Department of Horticulture, Faculty of Agriculture, Çukurova University, Balcalı, Sarıçam, Adana 01330, TurkeyThe optimization of tissue culture protocols for blueberry propagation is crucial to meet the increasing demand for disease-free planting materials. This study aimed to determine the effects of three auxins (IBA, IAA, and NAA) at different concentrations to enhance root and shoot development of two blueberry cultivars with different abilities to root, ‘Lanmei #1’and ‘Brightwell’. Results showed that NAA at 0.5 mg·L−1 significantly promoted shoot number and length in ‘Lanmei #1’ while ‘Brightwell’ showed a limited response. IAA had a variable impact on both shoot and root parameters, with ‘Lanmei #1’ showing more pronounced positive responses. IBA showed a differential response on root and shoot parameters in both cultivars, with ‘Lanmei #1’ showing a superior response for shoot number, length, root length, and overall plant vigor. Activated carbon had minimal effect on growth parameters across both cultivars. ‘Lanmei #1’ demonstrated a robust response to auxin treatments, suggesting it is more suitable for in vitro rooting. The present results confirm previous data on the different abilities of blueberry genotypes to root and the necessity of optimizing the rooting protocol depending on the cultivar.https://doi.org/10.2478/johr-2024-0016in vitro culturegrowth regulatorsmicropropagationrooting potential |
| spellingShingle | Zalt Najla Yasmin Samina Hamakhan Awara M. Polat Şule Yu Hong Ge Chunfeng Imrak Burhanettin Kafkas Ebru Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum) Journal of Horticultural Research in vitro culture growth regulators micropropagation rooting potential |
| title | Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum) |
| title_full | Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum) |
| title_fullStr | Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum) |
| title_full_unstemmed | Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum) |
| title_short | Effect of Auxins and Activated Carbon on In Vitro Rooting of Two Blueberry Cultivars (Vaccinium corymbosum) |
| title_sort | effect of auxins and activated carbon on in vitro rooting of two blueberry cultivars vaccinium corymbosum |
| topic | in vitro culture growth regulators micropropagation rooting potential |
| url | https://doi.org/10.2478/johr-2024-0016 |
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