The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population
Objective: Understanding the genetic basis of type 2 diabetes mellitus (T2DM) is essential in getting its etiology and designing effective preventive strategies. Evaluation of the association between the gene polymorphisms rs689A/T (INS) and rs757110C/A (ABCC8) and the susceptibility to T2DM within...
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Shahid Sadoughi University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Diabetes and Obesity |
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| Online Access: | http://ijdo.ssu.ac.ir/article-1-931-en.pdf |
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| author | Amin Haghighi Amaneh Javid Ali Khodadadian Mohammad Yahya Vahidi Mehrjardi |
| author_facet | Amin Haghighi Amaneh Javid Ali Khodadadian Mohammad Yahya Vahidi Mehrjardi |
| author_sort | Amin Haghighi |
| collection | DOAJ |
| description | Objective: Understanding the genetic basis of type 2 diabetes mellitus (T2DM) is essential in getting its etiology and designing effective preventive strategies. Evaluation of the association between the gene polymorphisms rs689A/T (INS) and rs757110C/A (ABCC8) and the susceptibility to T2DM within a group of individuals diagnosed with T2DM in Iran.
Materials and Methods: Blood samples were used for DNA extraction (200 with T2DM and 200 healthy controls). The quality and quantity of extracted DNA were assessed via ultraviolet spectrophotometry at 260 nm and 280 nm wavelengths. To identify specific alleles, primer sequences were manually designed using Primer3Plus, and the genotypes of rs689 (A>T) and rs757110 C>A were determined through ARMS-PCR. Statistical analysis was conducted using GraphPad Prism version 8.
Results: For rs689 individuals with the genotype (AA) were found to have a higher likelihood of developing diabetes (P= 0.001). Additionally, the frequency of the A allele was found to be higher in the patient group (0.13) compared to the control group (0.05). For rs757110 individuals with the genotype (CC) were found to have a higher likelihood of developing diabetes (P< 0.001). Additionally, the frequency of the C allele was found to be higher in the patient group (0.57) compared to the control group (0.39).
Conclusion: This study found that the frequency of AA and AT genotypes of rs689 and CC and CA genotypes of rs757110 are associated with T2DM risk. |
| format | Article |
| id | doaj-art-3a901f68891b4283843507a33e16face |
| institution | DOAJ |
| issn | 2008-6792 2345-2250 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Diabetes and Obesity |
| spelling | doaj-art-3a901f68891b4283843507a33e16face2025-08-20T02:59:50ZengShahid Sadoughi University of Medical SciencesIranian Journal of Diabetes and Obesity2008-67922345-22502025-02-011711927The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian PopulationAmin Haghighi0Amaneh Javid1Ali Khodadadian2Mohammad Yahya Vahidi Mehrjardi3 Department of Biology, Faculty of Engineering and Science, Science and Arts University, Yazd, Iran. Department of Biology, Faculty of Engineering and Science, Science and Arts University, Yazd, Iran. Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Diabetes Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Objective: Understanding the genetic basis of type 2 diabetes mellitus (T2DM) is essential in getting its etiology and designing effective preventive strategies. Evaluation of the association between the gene polymorphisms rs689A/T (INS) and rs757110C/A (ABCC8) and the susceptibility to T2DM within a group of individuals diagnosed with T2DM in Iran. Materials and Methods: Blood samples were used for DNA extraction (200 with T2DM and 200 healthy controls). The quality and quantity of extracted DNA were assessed via ultraviolet spectrophotometry at 260 nm and 280 nm wavelengths. To identify specific alleles, primer sequences were manually designed using Primer3Plus, and the genotypes of rs689 (A>T) and rs757110 C>A were determined through ARMS-PCR. Statistical analysis was conducted using GraphPad Prism version 8. Results: For rs689 individuals with the genotype (AA) were found to have a higher likelihood of developing diabetes (P= 0.001). Additionally, the frequency of the A allele was found to be higher in the patient group (0.13) compared to the control group (0.05). For rs757110 individuals with the genotype (CC) were found to have a higher likelihood of developing diabetes (P< 0.001). Additionally, the frequency of the C allele was found to be higher in the patient group (0.57) compared to the control group (0.39). Conclusion: This study found that the frequency of AA and AT genotypes of rs689 and CC and CA genotypes of rs757110 are associated with T2DM risk.http://ijdo.ssu.ac.ir/article-1-931-en.pdfdiabetesarms-pcrrs689rs757110 |
| spellingShingle | Amin Haghighi Amaneh Javid Ali Khodadadian Mohammad Yahya Vahidi Mehrjardi The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population Iranian Journal of Diabetes and Obesity diabetes arms-pcr rs689 rs757110 |
| title | The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population |
| title_full | The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population |
| title_fullStr | The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population |
| title_full_unstemmed | The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population |
| title_short | The impact of Polymorphisms of rs689 and rs757110 in Familial Type 2 Diabetes: A Case-Control Study in the Iranian Population |
| title_sort | impact of polymorphisms of rs689 and rs757110 in familial type 2 diabetes a case control study in the iranian population |
| topic | diabetes arms-pcr rs689 rs757110 |
| url | http://ijdo.ssu.ac.ir/article-1-931-en.pdf |
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