Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture
A model of the native absorption spectrum of the red seaweed Porphyridium purpureum culture was developed in this work. The mathematical model of each pigment is the sum of Gaussian curves. To level the light scattering, the spectra of the culture were recorded on a spectrophotometer with an integra...
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| Format: | Article |
| Language: | English |
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Samara National Research University
2024-04-01
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| Series: | Вестник Самарского университета: Естественнонаучная серия |
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| Online Access: | https://journals.ssau.ru/est/article/viewFile/27405/10535 |
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| author | D. N. Chernyshev V. S. Klochkova A. S. Lelekov |
| author_facet | D. N. Chernyshev V. S. Klochkova A. S. Lelekov |
| author_sort | D. N. Chernyshev |
| collection | DOAJ |
| description | A model of the native absorption spectrum of the red seaweed Porphyridium purpureum culture was developed in this work. The mathematical model of each pigment is the sum of Gaussian curves. To level the light scattering, the spectra of the culture were recorded on a spectrophotometer with an integrating sphere. To verify the model, a series of parallel measurements of photosynthetic pigment concentrations using standard biochemical methods and the Gaussian curve method were performed. It was shown that the proposed model with sufficient accuracy makes it possible to determine the concentration of the main photosynthetic pigments of Porphyridium purpureum culture without interfering with its growth processes. |
| format | Article |
| id | doaj-art-3a8ee126d90d44359d8d7014a4dc1682 |
| institution | Kabale University |
| issn | 2541-7525 2712-8954 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Samara National Research University |
| record_format | Article |
| series | Вестник Самарского университета: Естественнонаучная серия |
| spelling | doaj-art-3a8ee126d90d44359d8d7014a4dc16822025-08-20T03:48:06ZengSamara National Research UniversityВестник Самарского университета: Естественнонаучная серия2541-75252712-89542024-04-0130112213110.18287/2541-7525-2024-30-1-122-1318809Model of decomposition of the native absorption spectrum of Porphyridium purpureum cultureD. N. Chernyshev0https://orcid.org/0000-0002-8120-7382V. S. Klochkova1https://orcid.org/0000-0002-2120-9589A. S. Lelekov2https://orcid.org/0000-0003-1213-7963Sevastopol State UniversitySevastopol State UniversityA.O. Kovalevsky Institute of Biology of the Southern SeasA model of the native absorption spectrum of the red seaweed Porphyridium purpureum culture was developed in this work. The mathematical model of each pigment is the sum of Gaussian curves. To level the light scattering, the spectra of the culture were recorded on a spectrophotometer with an integrating sphere. To verify the model, a series of parallel measurements of photosynthetic pigment concentrations using standard biochemical methods and the Gaussian curve method were performed. It was shown that the proposed model with sufficient accuracy makes it possible to determine the concentration of the main photosynthetic pigments of Porphyridium purpureum culture without interfering with its growth processes.https://journals.ssau.ru/est/article/viewFile/27405/10535mathematical modelb-phycoerythrinchlorophyll acarotenoidsgauss curvesabsorption spectrum |
| spellingShingle | D. N. Chernyshev V. S. Klochkova A. S. Lelekov Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture Вестник Самарского университета: Естественнонаучная серия mathematical model b-phycoerythrin chlorophyll a carotenoids gauss curves absorption spectrum |
| title | Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture |
| title_full | Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture |
| title_fullStr | Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture |
| title_full_unstemmed | Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture |
| title_short | Model of decomposition of the native absorption spectrum of Porphyridium purpureum culture |
| title_sort | model of decomposition of the native absorption spectrum of porphyridium purpureum culture |
| topic | mathematical model b-phycoerythrin chlorophyll a carotenoids gauss curves absorption spectrum |
| url | https://journals.ssau.ru/est/article/viewFile/27405/10535 |
| work_keys_str_mv | AT dnchernyshev modelofdecompositionofthenativeabsorptionspectrumofporphyridiumpurpureumculture AT vsklochkova modelofdecompositionofthenativeabsorptionspectrumofporphyridiumpurpureumculture AT aslelekov modelofdecompositionofthenativeabsorptionspectrumofporphyridiumpurpureumculture |