In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
Myocardial infarction (MI) causes an extensive loss of heart muscle cells and leads to congestive heart disease (CAD), the leading cause of mortality and morbidity worldwide. Mesenchymal stromal cell- (MSC-) based cell therapy is a promising option to replace invasive interventions. However the opti...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7513252 |
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| author | Peter Szaraz Matthew Librach Leila Maghen Farwah Iqbal Tanya A. Barretto Shlomit Kenigsberg Andrée Gauthier-Fisher Clifford L. Librach |
| author_facet | Peter Szaraz Matthew Librach Leila Maghen Farwah Iqbal Tanya A. Barretto Shlomit Kenigsberg Andrée Gauthier-Fisher Clifford L. Librach |
| author_sort | Peter Szaraz |
| collection | DOAJ |
| description | Myocardial infarction (MI) causes an extensive loss of heart muscle cells and leads to congestive heart disease (CAD), the leading cause of mortality and morbidity worldwide. Mesenchymal stromal cell- (MSC-) based cell therapy is a promising option to replace invasive interventions. However the optimal cell type providing significant cardiac regeneration after MI is yet to be found. The aim of our study was to investigate the cardiomyogenic differentiation potential of first trimester human umbilical cord perivascular cells (FTM HUCPVCs), a novel, young source of immunoprivileged mesenchymal stromal cells. Based on the expression of cardiomyocyte markers (cTnT, MYH6, SIRPA, and CX43) FTM and term HUCPVCs achieved significantly increased cardiomyogenic differentiation compared to bone marrow MSCs, while their immunogenicity remained significantly lower as indicated by HLA-A and HLA-G expression and susceptibility to T cell mediated cytotoxicity. When applying aggregate-based differentiation, FTM HUCPVCs showed increased aggregate formation potential and generated contracting cells within 1 week of coculture, making them the first MSC type with this ability. Our results indicate that young FTM HUCPVCs have superior cardiomyogenic potential coupled with beneficial immunogenic properties when compared to MSCs of older tissue sources, suggesting that in vitro predifferentiation could be a potential strategy to increase their effectiveness in vivo. |
| format | Article |
| id | doaj-art-3a8db5f1f4e64135960eb3487b175ae5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3a8db5f1f4e64135960eb3487b175ae52025-02-03T01:12:58ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/75132527513252In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like CellsPeter Szaraz0Matthew Librach1Leila Maghen2Farwah Iqbal3Tanya A. Barretto4Shlomit Kenigsberg5Andrée Gauthier-Fisher6Clifford L. Librach7Create Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaCreate Fertility Centre, Toronto, ON, M5G 1N8, CanadaMyocardial infarction (MI) causes an extensive loss of heart muscle cells and leads to congestive heart disease (CAD), the leading cause of mortality and morbidity worldwide. Mesenchymal stromal cell- (MSC-) based cell therapy is a promising option to replace invasive interventions. However the optimal cell type providing significant cardiac regeneration after MI is yet to be found. The aim of our study was to investigate the cardiomyogenic differentiation potential of first trimester human umbilical cord perivascular cells (FTM HUCPVCs), a novel, young source of immunoprivileged mesenchymal stromal cells. Based on the expression of cardiomyocyte markers (cTnT, MYH6, SIRPA, and CX43) FTM and term HUCPVCs achieved significantly increased cardiomyogenic differentiation compared to bone marrow MSCs, while their immunogenicity remained significantly lower as indicated by HLA-A and HLA-G expression and susceptibility to T cell mediated cytotoxicity. When applying aggregate-based differentiation, FTM HUCPVCs showed increased aggregate formation potential and generated contracting cells within 1 week of coculture, making them the first MSC type with this ability. Our results indicate that young FTM HUCPVCs have superior cardiomyogenic potential coupled with beneficial immunogenic properties when compared to MSCs of older tissue sources, suggesting that in vitro predifferentiation could be a potential strategy to increase their effectiveness in vivo.http://dx.doi.org/10.1155/2016/7513252 |
| spellingShingle | Peter Szaraz Matthew Librach Leila Maghen Farwah Iqbal Tanya A. Barretto Shlomit Kenigsberg Andrée Gauthier-Fisher Clifford L. Librach In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells Stem Cells International |
| title | In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells |
| title_full | In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells |
| title_fullStr | In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells |
| title_full_unstemmed | In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells |
| title_short | In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells |
| title_sort | in vitro differentiation of first trimester human umbilical cord perivascular cells into contracting cardiomyocyte like cells |
| url | http://dx.doi.org/10.1155/2016/7513252 |
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