Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats
Abstract Painful diabetic neuropathy (PDN) is one of the most common complications of diabetes. Recent studies suggested that gut microbiota dysbiosis contributes to the development of PDN, but underlying mechanisms remain elusive. In this study, we found decreased probiotics generating bacteria suc...
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| Language: | English |
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BMC
2025-02-01
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| Series: | Journal of Neuroinflammation |
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| Online Access: | https://doi.org/10.1186/s12974-025-03352-3 |
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| author | Ye Jiang Jing Yang Min Wei Jiayin Shou Shixiong Shen Zhuoying Yu Zixian Zhang Jie Cai Yanhan Lyu Dongsheng Yang Yongzheng Han Jinpiao Zhu Zhigang Liu Daqing Ma Guo-gang Xing Min Li |
| author_facet | Ye Jiang Jing Yang Min Wei Jiayin Shou Shixiong Shen Zhuoying Yu Zixian Zhang Jie Cai Yanhan Lyu Dongsheng Yang Yongzheng Han Jinpiao Zhu Zhigang Liu Daqing Ma Guo-gang Xing Min Li |
| author_sort | Ye Jiang |
| collection | DOAJ |
| description | Abstract Painful diabetic neuropathy (PDN) is one of the most common complications of diabetes. Recent studies suggested that gut microbiota dysbiosis contributes to the development of PDN, but underlying mechanisms remain elusive. In this study, we found decreased probiotics generating bacteria such as Lactobacillus and Bifidobacterium strains in the PDN rats. Supplementation with multiple probiotics for 12 weeks alleviated pain, reversed nerve fiber lesions, and restored neuronal hyperexcitability. Probiotics administration effectively attenuated intestinal barrier impairment, reduced serum lipopolysaccharide and proinflammatory cytokines, and mitigated disruptions in the blood-nerve barrier. Furthermore, probiotics treatment inhibited the activation of the TLR4/MyD88/NF-κB signaling pathway and reduced proinflammatory cytokines in the sciatic nerve of the PDN rats. Together, our findings suggest that gut microbiota dysbiosis participates in PDN pathogenesis, and probiotics offer therapeutic potential via modulating the microbiota-gut-nerve axis. Graphical abstract |
| format | Article |
| id | doaj-art-3a846113040f4b43b88ff1add8866d0b |
| institution | Kabale University |
| issn | 1742-2094 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj-art-3a846113040f4b43b88ff1add8866d0b2025-02-09T12:48:10ZengBMCJournal of Neuroinflammation1742-20942025-02-0122111710.1186/s12974-025-03352-3Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in ratsYe Jiang0Jing Yang1Min Wei2Jiayin Shou3Shixiong Shen4Zhuoying Yu5Zixian Zhang6Jie Cai7Yanhan Lyu8Dongsheng Yang9Yongzheng Han10Jinpiao Zhu11Zhigang Liu12Daqing Ma13Guo-gang Xing14Min Li15Department of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalNeuroscience Research Institute, Peking UniversityNeuroscience Research Institute, Peking UniversityDepartment of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalDepartment of Anesthesiology, Peking University Third HospitalPerioperative and Systems Medicine Laboratory, Department of Anesthesiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthPerioperative and Systems Medicine Laboratory, Department of Anesthesiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthPerioperative and Systems Medicine Laboratory, Department of Anesthesiology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthNeuroscience Research Institute, Peking UniversityDepartment of Anesthesiology, Peking University Third HospitalAbstract Painful diabetic neuropathy (PDN) is one of the most common complications of diabetes. Recent studies suggested that gut microbiota dysbiosis contributes to the development of PDN, but underlying mechanisms remain elusive. In this study, we found decreased probiotics generating bacteria such as Lactobacillus and Bifidobacterium strains in the PDN rats. Supplementation with multiple probiotics for 12 weeks alleviated pain, reversed nerve fiber lesions, and restored neuronal hyperexcitability. Probiotics administration effectively attenuated intestinal barrier impairment, reduced serum lipopolysaccharide and proinflammatory cytokines, and mitigated disruptions in the blood-nerve barrier. Furthermore, probiotics treatment inhibited the activation of the TLR4/MyD88/NF-κB signaling pathway and reduced proinflammatory cytokines in the sciatic nerve of the PDN rats. Together, our findings suggest that gut microbiota dysbiosis participates in PDN pathogenesis, and probiotics offer therapeutic potential via modulating the microbiota-gut-nerve axis. Graphical abstracthttps://doi.org/10.1186/s12974-025-03352-3Painful diabetic neuropathyMicrobiota–gut–nerve axisProbioticsNeuroinflammation |
| spellingShingle | Ye Jiang Jing Yang Min Wei Jiayin Shou Shixiong Shen Zhuoying Yu Zixian Zhang Jie Cai Yanhan Lyu Dongsheng Yang Yongzheng Han Jinpiao Zhu Zhigang Liu Daqing Ma Guo-gang Xing Min Li Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats Journal of Neuroinflammation Painful diabetic neuropathy Microbiota–gut–nerve axis Probiotics Neuroinflammation |
| title | Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats |
| title_full | Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats |
| title_fullStr | Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats |
| title_full_unstemmed | Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats |
| title_short | Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats |
| title_sort | probiotics alleviate painful diabetic neuropathy by modulating the microbiota gut nerve axis in rats |
| topic | Painful diabetic neuropathy Microbiota–gut–nerve axis Probiotics Neuroinflammation |
| url | https://doi.org/10.1186/s12974-025-03352-3 |
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