First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China
Canine calicivirus (CaCV) belongs to the Caliciviridae family, which invades multiple host species. Notably, there are distinct serological and genetic differences between CaCV and other caliciviruses. However, the genome data for only 13 strains of CaCV have been recorded. Moreover, there have been...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1501632/full |
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| author | Sihan Li Liangyu Chu Yancheng Zhang Yaxuan Yu Guoqing Wang |
| author_facet | Sihan Li Liangyu Chu Yancheng Zhang Yaxuan Yu Guoqing Wang |
| author_sort | Sihan Li |
| collection | DOAJ |
| description | Canine calicivirus (CaCV) belongs to the Caliciviridae family, which invades multiple host species. Notably, there are distinct serological and genetic differences between CaCV and other caliciviruses. However, the genome data for only 13 strains of CaCV have been recorded. Moreover, there have been no reports on the CaCV genome in China. To understand the genetic characteristics of CaCV in China, this study tested CaCV in 52 canine nasal swab samples by RT–PCR and finally determined that two samples were positive for this virus. The complete genome sequences of both CaCV strains were obtained through sequencing, with a genomic length of 8,453 bp. The genomic sequences of the two Chinese CaCV strains presented 83.6% nucleotide similarity with each other but 71.6%−90.2% nucleotide similarity with previously reported CaCV strains, indicating that these two CaCV strains were genetically divergent. The subsequent sequence analysis indicated that these two strains possessed unique nucleotide sites in their genomic sequences. Phylogenetic analysis revealed that the Chinese CaCV strains belonged to different evolutionary branches. Overall, the present study provides new knowledge on the prevalence and genetic diversity of CaCV in China. |
| format | Article |
| id | doaj-art-3a80f23bbc4641efa2078058b31bdd3f |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-3a80f23bbc4641efa2078058b31bdd3f2025-08-20T02:49:52ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.15016321501632First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in ChinaSihan Li0Liangyu Chu1Yancheng Zhang2Yaxuan Yu3Guoqing Wang4College of Medicine and Biological Information Engineering, Northeastern University, Shengyang, ChinaCollege of Medicine and Biological Information Engineering, Northeastern University, Shengyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shengyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shengyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shengyang, ChinaCanine calicivirus (CaCV) belongs to the Caliciviridae family, which invades multiple host species. Notably, there are distinct serological and genetic differences between CaCV and other caliciviruses. However, the genome data for only 13 strains of CaCV have been recorded. Moreover, there have been no reports on the CaCV genome in China. To understand the genetic characteristics of CaCV in China, this study tested CaCV in 52 canine nasal swab samples by RT–PCR and finally determined that two samples were positive for this virus. The complete genome sequences of both CaCV strains were obtained through sequencing, with a genomic length of 8,453 bp. The genomic sequences of the two Chinese CaCV strains presented 83.6% nucleotide similarity with each other but 71.6%−90.2% nucleotide similarity with previously reported CaCV strains, indicating that these two CaCV strains were genetically divergent. The subsequent sequence analysis indicated that these two strains possessed unique nucleotide sites in their genomic sequences. Phylogenetic analysis revealed that the Chinese CaCV strains belonged to different evolutionary branches. Overall, the present study provides new knowledge on the prevalence and genetic diversity of CaCV in China.https://www.frontiersin.org/articles/10.3389/fvets.2024.1501632/fullcanine calicivirusvesivirusescompanion animalsgenetic characterizationphylogenetic analysis |
| spellingShingle | Sihan Li Liangyu Chu Yancheng Zhang Yaxuan Yu Guoqing Wang First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China Frontiers in Veterinary Science canine calicivirus vesiviruses companion animals genetic characterization phylogenetic analysis |
| title | First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China |
| title_full | First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China |
| title_fullStr | First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China |
| title_full_unstemmed | First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China |
| title_short | First detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in China |
| title_sort | first detection and characterization of genetically divergent canine calicivirus strains in domestic dogs in china |
| topic | canine calicivirus vesiviruses companion animals genetic characterization phylogenetic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1501632/full |
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