Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach
The West Nile virus (WNV) is a mosquito-borne virus of the Flaviviridae family that is transmitted through the mosquito-migratory bird-mosquito cycle. Currently, WNV infection is widespread in the Americas, Europe, and Africa, and is one of the most important global epidemic infectious diseases. Alt...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1468530/full |
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| author | Kunsheng Tao Chan He Tong Zhang Changguang Xiao Lifei Du Zongjie Li Donghua Shao Jianchao Wei Beibei Li Yafeng Qiu Zhiyong Ma Ke Liu |
| author_facet | Kunsheng Tao Chan He Tong Zhang Changguang Xiao Lifei Du Zongjie Li Donghua Shao Jianchao Wei Beibei Li Yafeng Qiu Zhiyong Ma Ke Liu |
| author_sort | Kunsheng Tao |
| collection | DOAJ |
| description | The West Nile virus (WNV) is a mosquito-borne virus of the Flaviviridae family that is transmitted through the mosquito-migratory bird-mosquito cycle. Currently, WNV infection is widespread in the Americas, Europe, and Africa, and is one of the most important global epidemic infectious diseases. Although migratory birds play an important role in the spread of WNV, monitoring of migratory birds carrying the WNV remains limited. Here, we developed a new nucleic acid test for detecting migratory birds carrying WNV, which uses amplicons of WNV to test fecal samples from migratory birds. This new method was validated by using full-length WNV genomic plasmid. With this amplicon method, we tested the migratory bird droppings collected in different locations. The results indicated that the positive rate of WNV nucleic acid in migratory bird droppings was over 39%, which provides clues to the fact that migratory birds may carry the WNV in Xinjiang, China. |
| format | Article |
| id | doaj-art-3a75234a23b34d53986234c1dbd9b4fb |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-3a75234a23b34d53986234c1dbd9b4fb2025-08-20T03:45:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-03-011610.3389/fmicb.2025.14685301468530Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approachKunsheng Tao0Chan He1Tong Zhang2Changguang Xiao3Lifei Du4Zongjie Li5Donghua Shao6Jianchao Wei7Beibei Li8Yafeng Qiu9Zhiyong Ma10Ke Liu11Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, ChinaThe West Nile virus (WNV) is a mosquito-borne virus of the Flaviviridae family that is transmitted through the mosquito-migratory bird-mosquito cycle. Currently, WNV infection is widespread in the Americas, Europe, and Africa, and is one of the most important global epidemic infectious diseases. Although migratory birds play an important role in the spread of WNV, monitoring of migratory birds carrying the WNV remains limited. Here, we developed a new nucleic acid test for detecting migratory birds carrying WNV, which uses amplicons of WNV to test fecal samples from migratory birds. This new method was validated by using full-length WNV genomic plasmid. With this amplicon method, we tested the migratory bird droppings collected in different locations. The results indicated that the positive rate of WNV nucleic acid in migratory bird droppings was over 39%, which provides clues to the fact that migratory birds may carry the WNV in Xinjiang, China.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1468530/fullWest Nile virusmigratory birdampliconfecal samplesepidemiology |
| spellingShingle | Kunsheng Tao Chan He Tong Zhang Changguang Xiao Lifei Du Zongjie Li Donghua Shao Jianchao Wei Beibei Li Yafeng Qiu Zhiyong Ma Ke Liu Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach Frontiers in Microbiology West Nile virus migratory bird amplicon fecal samples epidemiology |
| title | Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach |
| title_full | Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach |
| title_fullStr | Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach |
| title_full_unstemmed | Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach |
| title_short | Evidence of WNV infection in migratory birds passing through Xinjiang, China, using viral genome amplicon approach |
| title_sort | evidence of wnv infection in migratory birds passing through xinjiang china using viral genome amplicon approach |
| topic | West Nile virus migratory bird amplicon fecal samples epidemiology |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1468530/full |
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