Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei
Abstract Impatiens morsei is an annual or perennial flower that is a member of the Impatiens genus in the Balsaminaceae family, with pink flowers and dark purple spots on the vexil and wing petals, which is important for the study of new flower colors of Balsaminaceae. However, there has been no stu...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-93846-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849734922327031808 |
|---|---|
| author | Hai-Ge Liu Ting-Ting Feng Si-Yu Ren Jian-Yuan Yang Lu-Qiu Zhao Xiao-Li Zhang Mei-Juan Huang Hai-Quan Huang |
| author_facet | Hai-Ge Liu Ting-Ting Feng Si-Yu Ren Jian-Yuan Yang Lu-Qiu Zhao Xiao-Li Zhang Mei-Juan Huang Hai-Quan Huang |
| author_sort | Hai-Ge Liu |
| collection | DOAJ |
| description | Abstract Impatiens morsei is an annual or perennial flower that is a member of the Impatiens genus in the Balsaminaceae family, with pink flowers and dark purple spots on the vexil and wing petals, which is important for the study of new flower colors of Balsaminaceae. However, there has been no study on the flavonoid biosynthesis pathway and carotenoid biosynthesis pathway of I. morsei and the mechanism of its pigment formation is still unclear. Transcriptome sequencing of spotted and non-spotted regions was performed using the petals of I. morsei at full bloom as test material. The results showed that (1) After transcriptome sequencing, 39,917 unigene sequences and 39,110 differentially expressed genes were obtained, and the number of genes that are down-expressed was higher than genes that are up-expressed. (2) Three key genes in the flavonoid biosynthetic pathway, FLS, PAL, and CHS1, were screened out. Additionally, two key genes in the carotenoid biosynthetic pathway, ABA1 and CCS, were identified. Furthermore, two regulatory genes, MSI4 and CPC, were also selected.and analyzed the spatiotemporal expression patterns of these seven genes. The results of this study lay a theoretical foundation for the study of the formation mechanism of floral spots in I. morsei. |
| format | Article |
| id | doaj-art-3a7459c4f98043ccb34bc4e5e5aed0c9 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-3a7459c4f98043ccb34bc4e5e5aed0c92025-08-20T03:07:40ZengNature PortfolioScientific Reports2045-23222025-04-0115111610.1038/s41598-025-93846-0Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morseiHai-Ge Liu0Ting-Ting Feng1Si-Yu Ren2Jian-Yuan Yang3Lu-Qiu Zhao4Xiao-Li Zhang5Mei-Juan Huang6Hai-Quan Huang7College of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityCollege of Landscape Architecture and Horticulture Sciences, Southwest Forestry UniversityAbstract Impatiens morsei is an annual or perennial flower that is a member of the Impatiens genus in the Balsaminaceae family, with pink flowers and dark purple spots on the vexil and wing petals, which is important for the study of new flower colors of Balsaminaceae. However, there has been no study on the flavonoid biosynthesis pathway and carotenoid biosynthesis pathway of I. morsei and the mechanism of its pigment formation is still unclear. Transcriptome sequencing of spotted and non-spotted regions was performed using the petals of I. morsei at full bloom as test material. The results showed that (1) After transcriptome sequencing, 39,917 unigene sequences and 39,110 differentially expressed genes were obtained, and the number of genes that are down-expressed was higher than genes that are up-expressed. (2) Three key genes in the flavonoid biosynthetic pathway, FLS, PAL, and CHS1, were screened out. Additionally, two key genes in the carotenoid biosynthetic pathway, ABA1 and CCS, were identified. Furthermore, two regulatory genes, MSI4 and CPC, were also selected.and analyzed the spatiotemporal expression patterns of these seven genes. The results of this study lay a theoretical foundation for the study of the formation mechanism of floral spots in I. morsei.https://doi.org/10.1038/s41598-025-93846-0Impatiens morseiVariegationTranscriptome analysisGene expression |
| spellingShingle | Hai-Ge Liu Ting-Ting Feng Si-Yu Ren Jian-Yuan Yang Lu-Qiu Zhao Xiao-Li Zhang Mei-Juan Huang Hai-Quan Huang Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei Scientific Reports Impatiens morsei Variegation Transcriptome analysis Gene expression |
| title | Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei |
| title_full | Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei |
| title_fullStr | Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei |
| title_full_unstemmed | Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei |
| title_short | Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei |
| title_sort | transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in impatiens morsei |
| topic | Impatiens morsei Variegation Transcriptome analysis Gene expression |
| url | https://doi.org/10.1038/s41598-025-93846-0 |
| work_keys_str_mv | AT haigeliu transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT tingtingfeng transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT siyuren transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT jianyuanyang transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT luqiuzhao transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT xiaolizhang transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT meijuanhuang transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei AT haiquanhuang transcriptomeanalysisrevealstheexpressionofgenesinvolvedintheformationofpetalvariegationinimpatiensmorsei |