Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries
This study utilized DNA-encoded library (DEL) screening to identify novel inhibitors of carbonic anhydrase 9 (CA9), a key cancer target. Among the identified compounds, CA9–005 exhibited inhibitory activity and reduced cancer cell viability. Biolayer interferometry confirmed CA9–005's direct in...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625004382 |
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| author | Ju Ho Lee Sung Bum Park Byumseok Koh Jiwoo Kim Kyoung Jin Choi Jeonghui Im Byungho Lim Daesoo Kim Byungwook Lee Sunjong Yu Nam-Chul Cho Doyoun Kim Yuno Lee Hyun Jin Kim Ki Young Kim Jung-Nyoung Heo |
| author_facet | Ju Ho Lee Sung Bum Park Byumseok Koh Jiwoo Kim Kyoung Jin Choi Jeonghui Im Byungho Lim Daesoo Kim Byungwook Lee Sunjong Yu Nam-Chul Cho Doyoun Kim Yuno Lee Hyun Jin Kim Ki Young Kim Jung-Nyoung Heo |
| author_sort | Ju Ho Lee |
| collection | DOAJ |
| description | This study utilized DNA-encoded library (DEL) screening to identify novel inhibitors of carbonic anhydrase 9 (CA9), a key cancer target. Among the identified compounds, CA9–005 exhibited inhibitory activity and reduced cancer cell viability. Biolayer interferometry confirmed CA9–005's direct interaction with CA9, supported by structural modeling. These findings highlight the potential of DEL screening for drug discovery and establish CA9–005 as a anticancer candidate, warranting further structural and therapeutic optimization. |
| format | Article |
| id | doaj-art-3a73a80f82404bbca68b3a108e357cd5 |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-3a73a80f82404bbca68b3a108e357cd52025-08-20T03:59:31ZengElsevierResults in Chemistry2211-71562025-07-011610245510.1016/j.rechem.2025.102455Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-librariesJu Ho Lee0Sung Bum Park1Byumseok Koh2Jiwoo Kim3Kyoung Jin Choi4Jeonghui Im5Byungho Lim6Daesoo Kim7Byungwook Lee8Sunjong Yu9Nam-Chul Cho10Doyoun Kim11Yuno Lee12Hyun Jin Kim13Ki Young Kim14Jung-Nyoung Heo15Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Medicinal Chemistry & Pharmacology, Korea National University of Science & Technology (UST), Daejeon 34113, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 34134, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 34134, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Medicinal Chemistry & Pharmacology, Korea National University of Science & Technology (UST), Daejeon 34113, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaDigital Biotech Innovation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaDigital Biotech Innovation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of KoreaTherapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Medicinal Chemistry & Pharmacology, Korea National University of Science & Technology (UST), Daejeon 34113, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of Korea; Corresponding authors at: Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea.Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of Korea; Corresponding authors at: Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea.Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of Korea; Corresponding authors at: Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea.Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 34134, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of Korea; Corresponding authors at: Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea.Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea; Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 34134, Republic of Korea; Department of DEL Technology, KRICT, Daejeon 34114, Republic of Korea; Corresponding authors at: Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea.This study utilized DNA-encoded library (DEL) screening to identify novel inhibitors of carbonic anhydrase 9 (CA9), a key cancer target. Among the identified compounds, CA9–005 exhibited inhibitory activity and reduced cancer cell viability. Biolayer interferometry confirmed CA9–005's direct interaction with CA9, supported by structural modeling. These findings highlight the potential of DEL screening for drug discovery and establish CA9–005 as a anticancer candidate, warranting further structural and therapeutic optimization.http://www.sciencedirect.com/science/article/pii/S2211715625004382DNA-encoded librariesDELCarbonic anhydrase 9Screeningcancer inhibition |
| spellingShingle | Ju Ho Lee Sung Bum Park Byumseok Koh Jiwoo Kim Kyoung Jin Choi Jeonghui Im Byungho Lim Daesoo Kim Byungwook Lee Sunjong Yu Nam-Chul Cho Doyoun Kim Yuno Lee Hyun Jin Kim Ki Young Kim Jung-Nyoung Heo Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries Results in Chemistry DNA-encoded libraries DEL Carbonic anhydrase 9 Screening cancer inhibition |
| title | Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries |
| title_full | Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries |
| title_fullStr | Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries |
| title_full_unstemmed | Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries |
| title_short | Discovery of CA9–005 as a new carbonic anhydrase 9 inhibitor using a DNA encoded-libraries |
| title_sort | discovery of ca9 005 as a new carbonic anhydrase 9 inhibitor using a dna encoded libraries |
| topic | DNA-encoded libraries DEL Carbonic anhydrase 9 Screening cancer inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625004382 |
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