Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism
Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epi...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/908121 |
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| author | Qiang Lou Yijun Qi Yuanfang Ma Di Qu |
| author_facet | Qiang Lou Yijun Qi Yuanfang Ma Di Qu |
| author_sort | Qiang Lou |
| collection | DOAJ |
| description | Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/lonization mass spectrometry (MALDI-TOF-MS). Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated. The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted. The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS. |
| format | Article |
| id | doaj-art-3a68bae624384076ad6af24da549b845 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-3a68bae624384076ad6af24da549b8452025-02-03T01:12:49ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/908121908121Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose MetabolismQiang Lou0Yijun Qi1Yuanfang Ma2Di Qu3Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, ChinaLaboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, ChinaLaboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, ChinaKey laboratory of Medical Molecular Virology of Ministry of Education and Ministry of Public Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, 138 Yixueyuan Road, Shanghai, 200032, ChinaStaphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/lonization mass spectrometry (MALDI-TOF-MS). Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated. The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted. The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS.http://dx.doi.org/10.1155/2014/908121 |
| spellingShingle | Qiang Lou Yijun Qi Yuanfang Ma Di Qu Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism The Scientific World Journal |
| title | Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism |
| title_full | Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism |
| title_fullStr | Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism |
| title_full_unstemmed | Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism |
| title_short | Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism |
| title_sort | two component signal transduction system saers positively regulates staphylococcus epidermidis glucose metabolism |
| url | http://dx.doi.org/10.1155/2014/908121 |
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