An agarose spot assay for chemotactic invasion
The directed motility of cells toward the source of a soluble chemical (chemotaxis) plays a role in events ranging from immune function to cancer progression. Numerous chemotaxis assays are commonly employed, yet none provides an optimal combination of the relevant parameters. The ideal chemotaxis a...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2010-02-01
|
| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000113353 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850152046706032640 |
|---|---|
| author | Helen L. Wiggins Joshua Z. Rappoport |
| author_facet | Helen L. Wiggins Joshua Z. Rappoport |
| author_sort | Helen L. Wiggins |
| collection | DOAJ |
| description | The directed motility of cells toward the source of a soluble chemical (chemotaxis) plays a role in events ranging from immune function to cancer progression. Numerous chemotaxis assays are commonly employed, yet none provides an optimal combination of the relevant parameters. The ideal chemotaxis assay for use in the analysis of cells crawling across a planar surface should be cost-effective, simple to perform, and suitable for high-throughput multiplexing, as well as permit alteration of experimental conditions during cell motility. Here we describe a novel chemotaxis assay based upon the invasion of cells into agarose spots into which chemoattractants are suspended. Our studies demonstrate that this system assays chemotaxis and not chemokinesis, and provide proof-of-principle for drug screening studies as well as analysis through high-resolution cellular imaging. |
| format | Article |
| id | doaj-art-3a6741f60b994cd992c741eec0cfc4c8 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2010-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-3a6741f60b994cd992c741eec0cfc4c82025-08-20T02:26:05ZengTaylor & Francis GroupBioTechniques0736-62051940-98182010-02-0148212112410.2144/000113353An agarose spot assay for chemotactic invasionHelen L. Wiggins0Joshua Z. Rappoport11School of Biosciences, University of Birmingham, Edgbaston, UK1School of Biosciences, University of Birmingham, Edgbaston, UKThe directed motility of cells toward the source of a soluble chemical (chemotaxis) plays a role in events ranging from immune function to cancer progression. Numerous chemotaxis assays are commonly employed, yet none provides an optimal combination of the relevant parameters. The ideal chemotaxis assay for use in the analysis of cells crawling across a planar surface should be cost-effective, simple to perform, and suitable for high-throughput multiplexing, as well as permit alteration of experimental conditions during cell motility. Here we describe a novel chemotaxis assay based upon the invasion of cells into agarose spots into which chemoattractants are suspended. Our studies demonstrate that this system assays chemotaxis and not chemokinesis, and provide proof-of-principle for drug screening studies as well as analysis through high-resolution cellular imaging.https://www.future-science.com/doi/10.2144/000113353chemotaxiscell motilitymetastasis |
| spellingShingle | Helen L. Wiggins Joshua Z. Rappoport An agarose spot assay for chemotactic invasion BioTechniques chemotaxis cell motility metastasis |
| title | An agarose spot assay for chemotactic invasion |
| title_full | An agarose spot assay for chemotactic invasion |
| title_fullStr | An agarose spot assay for chemotactic invasion |
| title_full_unstemmed | An agarose spot assay for chemotactic invasion |
| title_short | An agarose spot assay for chemotactic invasion |
| title_sort | agarose spot assay for chemotactic invasion |
| topic | chemotaxis cell motility metastasis |
| url | https://www.future-science.com/doi/10.2144/000113353 |
| work_keys_str_mv | AT helenlwiggins anagarosespotassayforchemotacticinvasion AT joshuazrappoport anagarosespotassayforchemotacticinvasion AT helenlwiggins agarosespotassayforchemotacticinvasion AT joshuazrappoport agarosespotassayforchemotacticinvasion |