Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein
Wolbachia, an endosymbiotic bacterium found in most species of filarial parasites, is thought to play a significant role in inducing innate inflammatory responses in lymphatic filariasis patients. However, the Wolbachia-deriv...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2007/84318 |
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| author | Chantima Porksakorn Surang Nuchprayoon Kiwon Park Alan L. Scott |
| author_facet | Chantima Porksakorn Surang Nuchprayoon Kiwon Park Alan L. Scott |
| author_sort | Chantima Porksakorn |
| collection | DOAJ |
| description | Wolbachia, an endosymbiotic bacterium
found in most species of filarial parasites, is thought to play a significant
role in inducing innate inflammatory responses in lymphatic filariasis
patients. However, the Wolbachia-derived molecules
that are recognized by the innate immune system have not yet been
identified. In this study, we exposed the murine macrophage cell line
RAW 264.7 to a recombinant form of the major Wolbachia
surface protein (rWSP) to determine if WSP is capable of innately inducing
cytokine transcription. Interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) mRNAs were all
upregulated by the rWSP stimulation in a dose-dependant manner. TNF
transcription peaked at 3 hours, whereas IL-1β and IL-6 transcription peaked at 6 hours post-rWSP exposure. The
levels of innate cytokine expression induced by a high-dose (9.0 μg/mL) rWSP in the RAW 264.7 cells were comparable to the
levels induced by 0.1 μg/mL E. coli-derived lipopolysaccharides.
Pretreatment of the rWSP with
proteinase-K drastically reduced IL-1β, IL-6, and TNF transcription. However, the proinflammatory
response was not inhibited by polymyxin
B treatment. These results strongly suggest that the major
Wolbachia surface
protein molecule WSP is an important inducer of innate immune
responses during filarial infections. |
| format | Article |
| id | doaj-art-3a64814b7a85432d81fd5313d191ddb4 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-3a64814b7a85432d81fd5313d191ddb42025-02-03T05:47:10ZengWileyMediators of Inflammation0962-93511466-18612007-01-01200710.1155/2007/8431884318Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface ProteinChantima Porksakorn0Surang Nuchprayoon1Kiwon Park2Alan L. Scott3Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandLymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USADepartment of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USAWolbachia, an endosymbiotic bacterium found in most species of filarial parasites, is thought to play a significant role in inducing innate inflammatory responses in lymphatic filariasis patients. However, the Wolbachia-derived molecules that are recognized by the innate immune system have not yet been identified. In this study, we exposed the murine macrophage cell line RAW 264.7 to a recombinant form of the major Wolbachia surface protein (rWSP) to determine if WSP is capable of innately inducing cytokine transcription. Interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) mRNAs were all upregulated by the rWSP stimulation in a dose-dependant manner. TNF transcription peaked at 3 hours, whereas IL-1β and IL-6 transcription peaked at 6 hours post-rWSP exposure. The levels of innate cytokine expression induced by a high-dose (9.0 μg/mL) rWSP in the RAW 264.7 cells were comparable to the levels induced by 0.1 μg/mL E. coli-derived lipopolysaccharides. Pretreatment of the rWSP with proteinase-K drastically reduced IL-1β, IL-6, and TNF transcription. However, the proinflammatory response was not inhibited by polymyxin B treatment. These results strongly suggest that the major Wolbachia surface protein molecule WSP is an important inducer of innate immune responses during filarial infections.http://dx.doi.org/10.1155/2007/84318 |
| spellingShingle | Chantima Porksakorn Surang Nuchprayoon Kiwon Park Alan L. Scott Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein Mediators of Inflammation |
| title | Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein |
| title_full | Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein |
| title_fullStr | Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein |
| title_full_unstemmed | Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein |
| title_short | Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein |
| title_sort | proinflammatory cytokine gene expression by murine macrophages in response to brugia malayi wolbachia surface protein |
| url | http://dx.doi.org/10.1155/2007/84318 |
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