Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022
This study aimed to investigate the prevalence, antimicrobial resistance (AMR), and virulence characteristics of <i>Vibrio parahaemolyticus</i> (<i>V. parahaemolyticus</i>) isolated from olive flounder (<i>Paralichthys olivaceus</i>) and rockfish (<i>Sebaste...
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MDPI AG
2025-07-01
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| author | Jonghoon Lee Hansol Kim Haiseong Kang Yongchjun Park Insun Joo Hyochin Kim |
| author_facet | Jonghoon Lee Hansol Kim Haiseong Kang Yongchjun Park Insun Joo Hyochin Kim |
| author_sort | Jonghoon Lee |
| collection | DOAJ |
| description | This study aimed to investigate the prevalence, antimicrobial resistance (AMR), and virulence characteristics of <i>Vibrio parahaemolyticus</i> (<i>V. parahaemolyticus</i>) isolated from olive flounder (<i>Paralichthys olivaceus</i>) and rockfish (<i>Sebastes schlegelii</i>) sashimi samples sold in South Korea from 2021 to 2022. A total of 500 fish samples were analyzed, from which 17 <i>V. parahaemolyticus</i> isolates were obtained. Antibiotic susceptibility testing using the minimum inhibitory concentration method revealed that 58.8% (10/17) of the isolates exhibited resistance to ampicillin, indicating the potential for AMR transmission in seafood-associated pathogens. Whole-genome sequencing (WGS) and a polymerase chain reaction detected the presence of <i>tlh</i> and <i>trh</i> virulence genes in all isolates, suggesting their pathogenic potential. Although the overall isolation rate of <i>V. parahaemolyticus</i> was low, the presence of virulence and AMR genes indicates public health relevance associated with raw seafood consumption. The increasing consumer demand for raw fish, coupled with environmental changes such as rising ocean temperatures, underscores the necessity of continuous surveillance to prevent foodborne outbreaks. These findings emphasize the need for targeted AMR monitoring and further research to mitigate the dissemination of resistant <i>V. parahaemolyticus</i> strains and enhance seafood safety. |
| format | Article |
| id | doaj-art-3a5e84a70b134ff8908f50bb2b0b9327 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-3a5e84a70b134ff8908f50bb2b0b93272025-08-20T02:47:09ZengMDPI AGMicroorganisms2076-26072025-07-01137156610.3390/microorganisms13071566Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022Jonghoon Lee0Hansol Kim1Haiseong Kang2Yongchjun Park3Insun Joo4Hyochin Kim5Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of KoreaFood Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of KoreaFood Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of KoreaFood Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of KoreaFood Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of KoreaFood Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of KoreaThis study aimed to investigate the prevalence, antimicrobial resistance (AMR), and virulence characteristics of <i>Vibrio parahaemolyticus</i> (<i>V. parahaemolyticus</i>) isolated from olive flounder (<i>Paralichthys olivaceus</i>) and rockfish (<i>Sebastes schlegelii</i>) sashimi samples sold in South Korea from 2021 to 2022. A total of 500 fish samples were analyzed, from which 17 <i>V. parahaemolyticus</i> isolates were obtained. Antibiotic susceptibility testing using the minimum inhibitory concentration method revealed that 58.8% (10/17) of the isolates exhibited resistance to ampicillin, indicating the potential for AMR transmission in seafood-associated pathogens. Whole-genome sequencing (WGS) and a polymerase chain reaction detected the presence of <i>tlh</i> and <i>trh</i> virulence genes in all isolates, suggesting their pathogenic potential. Although the overall isolation rate of <i>V. parahaemolyticus</i> was low, the presence of virulence and AMR genes indicates public health relevance associated with raw seafood consumption. The increasing consumer demand for raw fish, coupled with environmental changes such as rising ocean temperatures, underscores the necessity of continuous surveillance to prevent foodborne outbreaks. These findings emphasize the need for targeted AMR monitoring and further research to mitigate the dissemination of resistant <i>V. parahaemolyticus</i> strains and enhance seafood safety.https://www.mdpi.com/2076-2607/13/7/1566<i>Vibrio parahaemolyticus</i>antimicrobial resistanceampicillinseafoodgastroenteritis |
| spellingShingle | Jonghoon Lee Hansol Kim Haiseong Kang Yongchjun Park Insun Joo Hyochin Kim Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022 Microorganisms <i>Vibrio parahaemolyticus</i> antimicrobial resistance ampicillin seafood gastroenteritis |
| title | Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022 |
| title_full | Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022 |
| title_fullStr | Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022 |
| title_full_unstemmed | Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022 |
| title_short | Antibiotic Resistance and Characteristics of <i>Vibrio parahaemolyticus</i> Isolated from Seafood Distributed in South Korea from 2021 to 2022 |
| title_sort | antibiotic resistance and characteristics of i vibrio parahaemolyticus i isolated from seafood distributed in south korea from 2021 to 2022 |
| topic | <i>Vibrio parahaemolyticus</i> antimicrobial resistance ampicillin seafood gastroenteritis |
| url | https://www.mdpi.com/2076-2607/13/7/1566 |
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