Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2
Oxidative stress poses a challenge to in vitro embryo culture. As a flavonoid, galangin (GAL) has been shown to have antioxidant effects, but the effect and antioxidant capacity of GAL in the in vitro development of porcine parthenogenetic embryos are still unknown. In this study, we demonstrated th...
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2025-07-01
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| Series: | Antioxidants |
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| author | Zhi-Chao Chi Shu-Ming Shi Li-Ying Liu Lin-Yi Qu Jing-Hang Li Guan-Lin Jia Yu-Yan He Lin-Xuan Li Yong-Xun Jin Ming-Jun Zhang Xian-Feng Yu |
| author_facet | Zhi-Chao Chi Shu-Ming Shi Li-Ying Liu Lin-Yi Qu Jing-Hang Li Guan-Lin Jia Yu-Yan He Lin-Xuan Li Yong-Xun Jin Ming-Jun Zhang Xian-Feng Yu |
| author_sort | Zhi-Chao Chi |
| collection | DOAJ |
| description | Oxidative stress poses a challenge to in vitro embryo culture. As a flavonoid, galangin (GAL) has been shown to have antioxidant effects, but the effect and antioxidant capacity of GAL in the in vitro development of porcine parthenogenetic embryos are still unknown. In this study, we demonstrated that 1 µM GAL significantly increased the blastocyst rate, decreased the accumulation of intracellular reactive oxygen species (ROS), increased the glutathione (GSH) level, and enhanced mitochondrial function in early porcine embryos. Nuclear factor erythroid-2-related factor 2 (<i>Nrf2</i>) was identified as the target gene of GAL via network pharmacology, and the transcript levels of related antioxidant enzymes (HO-1, NQO1, SOD2, and CAT) were found to be increased. Since Nrf2 has seven domains, we constructed Nrf2 mutants lacking different domains in vitro. We found that GAL specifically binds to the Neh1 domain of Nrf2. Subsequent embryonic experiments demonstrated that the antioxidant effect of GAL was abolished after Nrf2 deletion. These results suggest that GAL can directly bind to Nrf2 to regulate the level of oxidative stress and improve mitochondrial function in embryos. |
| format | Article |
| id | doaj-art-3a45029cfe17455b98de3e2fa6c37784 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-3a45029cfe17455b98de3e2fa6c377842025-08-20T03:36:10ZengMDPI AGAntioxidants2076-39212025-07-0114782210.3390/antiox14070822Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2Zhi-Chao Chi0Shu-Ming Shi1Li-Ying Liu2Lin-Yi Qu3Jing-Hang Li4Guan-Lin Jia5Yu-Yan He6Lin-Xuan Li7Yong-Xun Jin8Ming-Jun Zhang9Xian-Feng Yu10College of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaOxidative stress poses a challenge to in vitro embryo culture. As a flavonoid, galangin (GAL) has been shown to have antioxidant effects, but the effect and antioxidant capacity of GAL in the in vitro development of porcine parthenogenetic embryos are still unknown. In this study, we demonstrated that 1 µM GAL significantly increased the blastocyst rate, decreased the accumulation of intracellular reactive oxygen species (ROS), increased the glutathione (GSH) level, and enhanced mitochondrial function in early porcine embryos. Nuclear factor erythroid-2-related factor 2 (<i>Nrf2</i>) was identified as the target gene of GAL via network pharmacology, and the transcript levels of related antioxidant enzymes (HO-1, NQO1, SOD2, and CAT) were found to be increased. Since Nrf2 has seven domains, we constructed Nrf2 mutants lacking different domains in vitro. We found that GAL specifically binds to the Neh1 domain of Nrf2. Subsequent embryonic experiments demonstrated that the antioxidant effect of GAL was abolished after Nrf2 deletion. These results suggest that GAL can directly bind to Nrf2 to regulate the level of oxidative stress and improve mitochondrial function in embryos.https://www.mdpi.com/2076-3921/14/7/822galanginIVCpreimplantation embryoNrf2oxidative stressmitochondria |
| spellingShingle | Zhi-Chao Chi Shu-Ming Shi Li-Ying Liu Lin-Yi Qu Jing-Hang Li Guan-Lin Jia Yu-Yan He Lin-Xuan Li Yong-Xun Jin Ming-Jun Zhang Xian-Feng Yu Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2 Antioxidants galangin IVC preimplantation embryo Nrf2 oxidative stress mitochondria |
| title | Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2 |
| title_full | Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2 |
| title_fullStr | Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2 |
| title_full_unstemmed | Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2 |
| title_short | Galangin Regulates Oxidative Stress Levels in Porcine Embryos Through Interaction with the Neh1 Domain of Nrf2 |
| title_sort | galangin regulates oxidative stress levels in porcine embryos through interaction with the neh1 domain of nrf2 |
| topic | galangin IVC preimplantation embryo Nrf2 oxidative stress mitochondria |
| url | https://www.mdpi.com/2076-3921/14/7/822 |
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