Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches
In agricultural systems, interactions between plants and microorganisms are important to maintaining production and profitability. In this study, bacterial communities in floodwaters of rice fields were monitored during the vegetative and reproductive stages of rice plant development using 16S ampli...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/6280484 |
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| _version_ | 1850157282865709056 |
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| author | Michele Pittol Erin Scully Daniel Miller Lisa Durso Lidia Mariana Fiuza Victor Hugo Valiati |
| author_facet | Michele Pittol Erin Scully Daniel Miller Lisa Durso Lidia Mariana Fiuza Victor Hugo Valiati |
| author_sort | Michele Pittol |
| collection | DOAJ |
| description | In agricultural systems, interactions between plants and microorganisms are important to maintaining production and profitability. In this study, bacterial communities in floodwaters of rice fields were monitored during the vegetative and reproductive stages of rice plant development using 16S amplicon sequencing. The study was conducted in the south of Brazil, during the crop years 2011/12 and 2012/13. Comparative analyses showed strong differences between the communities of floodwaters associated with the two developmental stages. During the vegetative stage, 1551 operational taxonomic units (OTUs) were detected, while less than half that number (603) were identified in the reproductive stage. The higher bacterial richness observed in floodwater collected during the vegetative stage may have been favored by the higher concentration of nutrients, such as potassium, due to rhizodeposition and fertilizer application. Eighteen bacterial phyla were identified in both samples. Both communities were dominated by Gammaproteobacteria. In the vegetative stage, Alphaproteobacteria and Betaproteobacteria were more abundant and, in contrast, Bacilli and Clostridia were the more dominant classes in the reproductive stage. The major bacterial taxa identified have been previously identified as important colonizers of rice fields. The richness and composition of bacterial communities over cultivation time may contribute to the sustainability of the crop. |
| format | Article |
| id | doaj-art-3a3e695f28c148a38d010a1d58360e6e |
| institution | OA Journals |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-3a3e695f28c148a38d010a1d58360e6e2025-08-20T02:24:14ZengWileyInternational Journal of Microbiology1687-918X1687-91982018-01-01201810.1155/2018/62804846280484Bacterial Community of the Rice Floodwater Using Cultivation-Independent ApproachesMichele Pittol0Erin Scully1Daniel Miller2Lisa Durso3Lidia Mariana Fiuza4Victor Hugo Valiati5Programa de Pós-Graduação em Biologia, Universidade do Vale do Rio dos Sinos (UNISINOS), 950 Unisinos Avenue, São Leopoldo, RS, BrazilUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), Center for Grain and Animal Health Research, Stored Product Insect and Engineering Research Unit (SPIERU), 1515 College Ave., Manhattan, KS, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), Agroecosystem Management Research Unit (AMRU), 251 Filley Hall, UNL East Campus, Lincoln, NE, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), Agroecosystem Management Research Unit (AMRU), 251 Filley Hall, UNL East Campus, Lincoln, NE, USAPrograma de Pós-Graduação em Biologia, Universidade do Vale do Rio dos Sinos (UNISINOS), 950 Unisinos Avenue, São Leopoldo, RS, BrazilPrograma de Pós-Graduação em Biologia, Universidade do Vale do Rio dos Sinos (UNISINOS), 950 Unisinos Avenue, São Leopoldo, RS, BrazilIn agricultural systems, interactions between plants and microorganisms are important to maintaining production and profitability. In this study, bacterial communities in floodwaters of rice fields were monitored during the vegetative and reproductive stages of rice plant development using 16S amplicon sequencing. The study was conducted in the south of Brazil, during the crop years 2011/12 and 2012/13. Comparative analyses showed strong differences between the communities of floodwaters associated with the two developmental stages. During the vegetative stage, 1551 operational taxonomic units (OTUs) were detected, while less than half that number (603) were identified in the reproductive stage. The higher bacterial richness observed in floodwater collected during the vegetative stage may have been favored by the higher concentration of nutrients, such as potassium, due to rhizodeposition and fertilizer application. Eighteen bacterial phyla were identified in both samples. Both communities were dominated by Gammaproteobacteria. In the vegetative stage, Alphaproteobacteria and Betaproteobacteria were more abundant and, in contrast, Bacilli and Clostridia were the more dominant classes in the reproductive stage. The major bacterial taxa identified have been previously identified as important colonizers of rice fields. The richness and composition of bacterial communities over cultivation time may contribute to the sustainability of the crop.http://dx.doi.org/10.1155/2018/6280484 |
| spellingShingle | Michele Pittol Erin Scully Daniel Miller Lisa Durso Lidia Mariana Fiuza Victor Hugo Valiati Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches International Journal of Microbiology |
| title | Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches |
| title_full | Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches |
| title_fullStr | Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches |
| title_full_unstemmed | Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches |
| title_short | Bacterial Community of the Rice Floodwater Using Cultivation-Independent Approaches |
| title_sort | bacterial community of the rice floodwater using cultivation independent approaches |
| url | http://dx.doi.org/10.1155/2018/6280484 |
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