Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i>
This study represents the first investigation into the ultrasonic and microwave extraction of bioactive metabolites from <i>Jania rubens (J. rubens)</i> (red seaweed) and <i>Sargassum. muticum</i> (<i>S. muticum</i>) (brown seaweed), with a focus on their biologic...
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2024-11-01
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| author | Kahina Hamamouche Zoubida Elhadj Latifa Khattabi Wafa Zahnit Brahim Djemoui Omar Kharoubi Walid Boussebaa Mouhamed Bouderballa Mohammed EL Moustapha Kallouche Sabry M. Attia Sheikh F. Ahmad Maria Atanassova Mohammed Messaoudi |
| author_facet | Kahina Hamamouche Zoubida Elhadj Latifa Khattabi Wafa Zahnit Brahim Djemoui Omar Kharoubi Walid Boussebaa Mouhamed Bouderballa Mohammed EL Moustapha Kallouche Sabry M. Attia Sheikh F. Ahmad Maria Atanassova Mohammed Messaoudi |
| author_sort | Kahina Hamamouche |
| collection | DOAJ |
| description | This study represents the first investigation into the ultrasonic and microwave extraction of bioactive metabolites from <i>Jania rubens (J. rubens)</i> (red seaweed) and <i>Sargassum. muticum</i> (<i>S. muticum</i>) (brown seaweed), with a focus on their biological activities. The research compares ultrasound-assisted extraction (UAE) with microwave-assisted extraction (MAE) utilizing a hydromethanolic solvent to evaluate their effects on these seaweeds’ bioactive compounds and biological activities. The assessment included a series of antioxidant essays: DPPH, ABTS, phenanthroline, and total antioxidant capacity, followed by enzyme inhibition activities: alpha-amylase and urease. Results revealed significant proportions of phenolic compounds, ranging from 48.31 ± 0.32 to 74.42 ± 0.80 μg GAE/mg, depending on the extraction method. The extracts demonstrated a high antioxidant activity, with IC<sub>50</sub> values ranging from 26.58 ± 0.39 to 87.55 ± 0.69 μg/mL. Notably, the MAE extract of <i>S. muticum</i> showed a value of 48.11 ± 2.75 μg/mL for alpha-amylase inhibition, which is strictly superior to the reference acarbose with an IC<sub>50</sub> equal to 3431.01 μg/mL. UPLC-ESI-MS/MS analysis identified 14 bioactive compounds. The proportion of riboflavin with MAE was 70.58% and 59.11% for <i>J. rubens</i> and <i>S. muticum</i> fractions, respectively. These findings underscore the critical influence of extraction technique selection on bioactive compounds’ yield and efficiency, highlighting the potential of algal biomass as a sustainable alternative in various applications. |
| format | Article |
| id | doaj-art-3a35599c71d042ce9a044183b39c06e2 |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj-art-3a35599c71d042ce9a044183b39c06e22025-08-20T02:00:47ZengMDPI AGMarine Drugs1660-33972024-11-01221253010.3390/md22120530Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i>Kahina Hamamouche0Zoubida Elhadj1Latifa Khattabi2Wafa Zahnit3Brahim Djemoui4Omar Kharoubi5Walid Boussebaa6Mouhamed Bouderballa7Mohammed EL Moustapha Kallouche8Sabry M. Attia9Sheikh F. Ahmad10Maria Atanassova11Mohammed Messaoudi12Environmental Monitoring Network Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, AlgeriaLaboratory for Environmental and Materials Sciences Studies, Ahmed Ben Bella University, Oran 31000, AlgeriaBiotechnology Research Center-C.R.B.T, Constantine 25017, AlgeriaDepartment of Chemistry, Faculty of Sciences, University of Ferhat ABBAS Setif 1, El Bez 19000, AlgeriaLaboratory of Excellence in Applied Organic Synthesis, Faculty of Exact and Applied Sciences, Ahmed Ben Bella University, Oran 31000, AlgeriaLaboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, AlgeriaScientific and Technical Research Center in Physico-Chemical Analysis CRAPC BP384, Bou-Ismail 42004, AlgeriaEnvironmental Monitoring Network Laboratory, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, AlgeriaEnvironmental Monitoring Network Laboratory, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, AlgeriaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaScientific Consulting, Chemical Engineering, University of Chemical Technology and Metallurgy, 1734 Sofia, BulgariaNuclear Research Centre of Birine, Ain Oussera 17200, AlgeriaThis study represents the first investigation into the ultrasonic and microwave extraction of bioactive metabolites from <i>Jania rubens (J. rubens)</i> (red seaweed) and <i>Sargassum. muticum</i> (<i>S. muticum</i>) (brown seaweed), with a focus on their biological activities. The research compares ultrasound-assisted extraction (UAE) with microwave-assisted extraction (MAE) utilizing a hydromethanolic solvent to evaluate their effects on these seaweeds’ bioactive compounds and biological activities. The assessment included a series of antioxidant essays: DPPH, ABTS, phenanthroline, and total antioxidant capacity, followed by enzyme inhibition activities: alpha-amylase and urease. Results revealed significant proportions of phenolic compounds, ranging from 48.31 ± 0.32 to 74.42 ± 0.80 μg GAE/mg, depending on the extraction method. The extracts demonstrated a high antioxidant activity, with IC<sub>50</sub> values ranging from 26.58 ± 0.39 to 87.55 ± 0.69 μg/mL. Notably, the MAE extract of <i>S. muticum</i> showed a value of 48.11 ± 2.75 μg/mL for alpha-amylase inhibition, which is strictly superior to the reference acarbose with an IC<sub>50</sub> equal to 3431.01 μg/mL. UPLC-ESI-MS/MS analysis identified 14 bioactive compounds. The proportion of riboflavin with MAE was 70.58% and 59.11% for <i>J. rubens</i> and <i>S. muticum</i> fractions, respectively. These findings underscore the critical influence of extraction technique selection on bioactive compounds’ yield and efficiency, highlighting the potential of algal biomass as a sustainable alternative in various applications.https://www.mdpi.com/1660-3397/22/12/530<i>Jania rubens</i><i>Sargassum muticum</i>ultrasound assisted extractionmicrowave-assisted extractionLC-MS analysisbioactive compounds |
| spellingShingle | Kahina Hamamouche Zoubida Elhadj Latifa Khattabi Wafa Zahnit Brahim Djemoui Omar Kharoubi Walid Boussebaa Mouhamed Bouderballa Mohammed EL Moustapha Kallouche Sabry M. Attia Sheikh F. Ahmad Maria Atanassova Mohammed Messaoudi Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i> Marine Drugs <i>Jania rubens</i> <i>Sargassum muticum</i> ultrasound assisted extraction microwave-assisted extraction LC-MS analysis bioactive compounds |
| title | Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i> |
| title_full | Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i> |
| title_fullStr | Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i> |
| title_full_unstemmed | Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i> |
| title_short | Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i> |
| title_sort | impact of ultrasound and microwave assisted extraction on bioactive compounds and biological activities of i jania rubens i and i sargassum muticum i |
| topic | <i>Jania rubens</i> <i>Sargassum muticum</i> ultrasound assisted extraction microwave-assisted extraction LC-MS analysis bioactive compounds |
| url | https://www.mdpi.com/1660-3397/22/12/530 |
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