Evidence for the Essential Arginine and Histidine Residues in Catalytic Activity of Glucose 6-Phosphate Dehydrogenase from Streptomyces aureofaciens

Evidence for the Essential Arginine and Histidine Residues in Catalytic Activity of Glucose 6-Phosphate Dehydrogenase from Streptomyces aureofaciens

Glucose 6-phosphate dehydrogenase (G6PD) was purified from Streptomyces aureofaciens and inactivated with butanedione and diethylpyrocarbonate. Incubation of the enzyme with butanedione resulted in a rapid activity loss (80%) within 5 min, followed by a slow phase using a molar ratio to enzyme conce...

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Bibliographic Details
Format: Article
Language:English
Published: University of Tehran 2005-03-01
Series:Journal of Sciences, Islamic Republic of Iran
Online Access:https://jsciences.ut.ac.ir/article_31621_9113301d6db6f4521430630c7ae3c7ec.pdf
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