Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>)
Avian pox is a globally spread viral disease affecting a wide spectrum of wild and domesticated bird species. The disease is caused by a diverse group of large DNA viruses, namely, avipoxviruses (genus <i>Avipoxvirus</i>, family <i>Poxviridae</i>). In this study, gross pathol...
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2024-12-01
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| author | Eszter Kaszab Endre Sós Krisztina Bali Viktória Sós-Koroknai Edina Perge Krisztina Ursu Szilvia Marton Márton Hoitsy Gábor Kemenesi Krisztián Bányai |
| author_facet | Eszter Kaszab Endre Sós Krisztina Bali Viktória Sós-Koroknai Edina Perge Krisztina Ursu Szilvia Marton Márton Hoitsy Gábor Kemenesi Krisztián Bányai |
| author_sort | Eszter Kaszab |
| collection | DOAJ |
| description | Avian pox is a globally spread viral disease affecting a wide spectrum of wild and domesticated bird species. The disease is caused by a diverse group of large DNA viruses, namely, avipoxviruses (genus <i>Avipoxvirus</i>, family <i>Poxviridae</i>). In this study, gross pathological examination and histopathological examination of skin lesions and several organs suggested acute poxvirus infection of a Eurasian crane (<i>Grus grus</i>, Linnaeus, 1758). Avipoxvirus infection was confirmed by testing wart-like lesions via gene-specific PCR assay and sequencing the obtained amplicon. Phylogenetic analysis of the gene encoding the DNA polymerase revealed that the crane poxvirus clustered in clade A, subclade A3. A large fragment of the poxvirus genome (306,477 bp in length) was assembled from the DNA of a skin specimen. Our study reaffirms previous findings that even complex virus genomes can be determined from a metagenomic assemblage generated directly from avian tissue samples without prior virus isolation, a promising approach for the epidemiologic surveillance of avipoxvirus infections in wild birds and domestic poultry. |
| format | Article |
| id | doaj-art-3a29e8c10f5d4a4986f909d875ac7493 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-3a29e8c10f5d4a4986f909d875ac74932025-01-10T13:13:56ZengMDPI AGAnimals2076-26152024-12-011516010.3390/ani15010060Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>)Eszter Kaszab0Endre Sós1Krisztina Bali2Viktória Sós-Koroknai3Edina Perge4Krisztina Ursu5Szilvia Marton6Márton Hoitsy7Gábor Kemenesi8Krisztián Bányai9HUN-REN Veterinary Medical Research Institute, Tábornok u. 2., H-1143 Budapest, HungaryBudapest Zoo and Botanical Garden, Állatkerti krt. 6-12, H-1146 Budapest, HungaryHUN-REN Veterinary Medical Research Institute, Tábornok u. 2., H-1143 Budapest, HungaryBudapest Zoo and Botanical Garden, Állatkerti krt. 6-12, H-1146 Budapest, HungaryMatrix Histopathology Service, Váci út 174, H-1085 Budapest, HungaryVeterinary Diagnostic Directorate, National Food Chain Safety Office, H-1024 Budapest, HungaryHUN-REN Veterinary Medical Research Institute, Tábornok u. 2., H-1143 Budapest, HungaryBudapest Zoo and Botanical Garden, Állatkerti krt. 6-12, H-1146 Budapest, HungaryInstitute of Biology, Faculty of Sciences, University of Pécs, H-7624 Pécs, HungaryHUN-REN Veterinary Medical Research Institute, Tábornok u. 2., H-1143 Budapest, HungaryAvian pox is a globally spread viral disease affecting a wide spectrum of wild and domesticated bird species. The disease is caused by a diverse group of large DNA viruses, namely, avipoxviruses (genus <i>Avipoxvirus</i>, family <i>Poxviridae</i>). In this study, gross pathological examination and histopathological examination of skin lesions and several organs suggested acute poxvirus infection of a Eurasian crane (<i>Grus grus</i>, Linnaeus, 1758). Avipoxvirus infection was confirmed by testing wart-like lesions via gene-specific PCR assay and sequencing the obtained amplicon. Phylogenetic analysis of the gene encoding the DNA polymerase revealed that the crane poxvirus clustered in clade A, subclade A3. A large fragment of the poxvirus genome (306,477 bp in length) was assembled from the DNA of a skin specimen. Our study reaffirms previous findings that even complex virus genomes can be determined from a metagenomic assemblage generated directly from avian tissue samples without prior virus isolation, a promising approach for the epidemiologic surveillance of avipoxvirus infections in wild birds and domestic poultry.https://www.mdpi.com/2076-2615/15/1/60avipoxvirusgenome sequencingphylogenetic analysis |
| spellingShingle | Eszter Kaszab Endre Sós Krisztina Bali Viktória Sós-Koroknai Edina Perge Krisztina Ursu Szilvia Marton Márton Hoitsy Gábor Kemenesi Krisztián Bányai Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>) Animals avipoxvirus genome sequencing phylogenetic analysis |
| title | Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>) |
| title_full | Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>) |
| title_fullStr | Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>) |
| title_full_unstemmed | Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>) |
| title_short | Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (<i>Grus grus</i>) |
| title_sort | near complete avipoxvirus genome assembled from skin lesions of dead eurasian crane i grus grus i |
| topic | avipoxvirus genome sequencing phylogenetic analysis |
| url | https://www.mdpi.com/2076-2615/15/1/60 |
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