In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication
Background: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has become one of the biggest health problems due to the lack of knowledge about effective therapy. Purpose: Some scientific studies have shown that essential oils (EOs) have anti-inflammatory, immunomod...
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Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324000435 |
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| author | Karelle Contant Shiv Shankar Monique Lacroix |
| author_facet | Karelle Contant Shiv Shankar Monique Lacroix |
| author_sort | Karelle Contant |
| collection | DOAJ |
| description | Background: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has become one of the biggest health problems due to the lack of knowledge about effective therapy. Purpose: Some scientific studies have shown that essential oils (EOs) have anti-inflammatory, immunomodulatory, and antiviral properties. Study design: This study demonstrated the potential antiviral activity of EOs in emulsion and in vapor forms to reduce the replication of MHV-A59, a murine surrogate of SARS-CoV-2. Methods: In the present study, 32 EOs were screened in vitro against MHV-A59 on DBT cells using plaque assay. EOs in emulsions were applied at their maximum noncytotoxic concentrations to MHV-A59 after penetration of the viruses into the host cells for 1 h during intracellular virus replication. Results: Monarda didyma at 5000 µg/ml showed a reduction of 100% of viral plaque. Tanacetum annuum at 10000 µg/ml inhibited 5.03-log MHV-A59. Beyond 4-log, the drug can be qualified as an antiviral according to the guidelines of ICH. In vapor form, none of the EOs showed potential inhibitory effects against MHV-A59. Conclusion: Results demonstrated that all 32 undiluted EOs, incubated with MHV-A59 for 30 min, had a ≤1.09-log inactivation compared to an untreated virus. The findings of this study may provide proof-of-concept and insight into related trials. |
| format | Article |
| id | doaj-art-3a2127042c1046569282b0b8e345d7f1 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj-art-3a2127042c1046569282b0b8e345d7f12025-02-10T04:35:02ZengElsevierPhytomedicine Plus2667-03132025-02-0151100566In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replicationKarelle Contant0Shiv Shankar1Monique Lacroix2Armand-Frappier Santé Biotechnologie Center, Institut national de la recherche scientifique, Research Laboratories in Sciences, Applied to Food, Canadian Irradiation Centre, 531 Boulevard des Prairies, Laval, Québec, H7V 1B7, Canada; Institut sur la nutrition et les aliments fonctionnels, 2440 Boulevard Hochelaga Suite 1710, Québec, G1V 0A6, CanadaArmand-Frappier Santé Biotechnologie Center, Institut national de la recherche scientifique, Research Laboratories in Sciences, Applied to Food, Canadian Irradiation Centre, 531 Boulevard des Prairies, Laval, Québec, H7V 1B7, Canada; Institut sur la nutrition et les aliments fonctionnels, 2440 Boulevard Hochelaga Suite 1710, Québec, G1V 0A6, CanadaArmand-Frappier Santé Biotechnologie Center, Institut national de la recherche scientifique, Research Laboratories in Sciences, Applied to Food, Canadian Irradiation Centre, 531 Boulevard des Prairies, Laval, Québec, H7V 1B7, Canada; Institut sur la nutrition et les aliments fonctionnels, 2440 Boulevard Hochelaga Suite 1710, Québec, G1V 0A6, Canada; Corresponding author.Background: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has become one of the biggest health problems due to the lack of knowledge about effective therapy. Purpose: Some scientific studies have shown that essential oils (EOs) have anti-inflammatory, immunomodulatory, and antiviral properties. Study design: This study demonstrated the potential antiviral activity of EOs in emulsion and in vapor forms to reduce the replication of MHV-A59, a murine surrogate of SARS-CoV-2. Methods: In the present study, 32 EOs were screened in vitro against MHV-A59 on DBT cells using plaque assay. EOs in emulsions were applied at their maximum noncytotoxic concentrations to MHV-A59 after penetration of the viruses into the host cells for 1 h during intracellular virus replication. Results: Monarda didyma at 5000 µg/ml showed a reduction of 100% of viral plaque. Tanacetum annuum at 10000 µg/ml inhibited 5.03-log MHV-A59. Beyond 4-log, the drug can be qualified as an antiviral according to the guidelines of ICH. In vapor form, none of the EOs showed potential inhibitory effects against MHV-A59. Conclusion: Results demonstrated that all 32 undiluted EOs, incubated with MHV-A59 for 30 min, had a ≤1.09-log inactivation compared to an untreated virus. The findings of this study may provide proof-of-concept and insight into related trials.http://www.sciencedirect.com/science/article/pii/S2667031324000435Essential oilsMurine coronavirusViral inhibitionAntiviral activityPlaque assay |
| spellingShingle | Karelle Contant Shiv Shankar Monique Lacroix In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication Phytomedicine Plus Essential oils Murine coronavirus Viral inhibition Antiviral activity Plaque assay |
| title | In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication |
| title_full | In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication |
| title_fullStr | In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication |
| title_full_unstemmed | In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication |
| title_short | In vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus A-59 replication |
| title_sort | in vitro study on the inhibitory effect of various essential oils against murine coronavirus mouse hepatitis virus a 59 replication |
| topic | Essential oils Murine coronavirus Viral inhibition Antiviral activity Plaque assay |
| url | http://www.sciencedirect.com/science/article/pii/S2667031324000435 |
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