ROCK1 and LIMK2 interact in spread but not blebbing cancer cells.
Cancer cells migrating within a 3D microenvironment are able to adopt either a mesenchymal or amoeboid mode of migration. Amoeboid migration is characterised by membrane blebbing that is dependent on the Rho effectors, ROCK1/2. We identify LIMK2 as the preferred substrate for ROCK1 but find that LIM...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003398&type=printable |
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| _version_ | 1849687097418448896 |
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| author | Kerry F Shea Claire M Wells Andrew P Garner Gareth E Jones |
| author_facet | Kerry F Shea Claire M Wells Andrew P Garner Gareth E Jones |
| author_sort | Kerry F Shea |
| collection | DOAJ |
| description | Cancer cells migrating within a 3D microenvironment are able to adopt either a mesenchymal or amoeboid mode of migration. Amoeboid migration is characterised by membrane blebbing that is dependent on the Rho effectors, ROCK1/2. We identify LIMK2 as the preferred substrate for ROCK1 but find that LIMK2 did not induce membrane blebbing, suggesting that a LIMK2 pathway is not involved in amoeboid-mode migration. In support of this hypothesis, novel FRET data demonstrate a direct interaction between ROCK1 and LIMK2 in polarised but not blebbing cells. Our results point to a specific role for the ROCK1:LIMK2 pathway in mesenchymal-mode migration. |
| format | Article |
| id | doaj-art-3a1e32c7a9ac4300a0aef830a2d947d7 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-3a1e32c7a9ac4300a0aef830a2d947d72025-08-20T03:22:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01310e339810.1371/journal.pone.0003398ROCK1 and LIMK2 interact in spread but not blebbing cancer cells.Kerry F SheaClaire M WellsAndrew P GarnerGareth E JonesCancer cells migrating within a 3D microenvironment are able to adopt either a mesenchymal or amoeboid mode of migration. Amoeboid migration is characterised by membrane blebbing that is dependent on the Rho effectors, ROCK1/2. We identify LIMK2 as the preferred substrate for ROCK1 but find that LIMK2 did not induce membrane blebbing, suggesting that a LIMK2 pathway is not involved in amoeboid-mode migration. In support of this hypothesis, novel FRET data demonstrate a direct interaction between ROCK1 and LIMK2 in polarised but not blebbing cells. Our results point to a specific role for the ROCK1:LIMK2 pathway in mesenchymal-mode migration.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003398&type=printable |
| spellingShingle | Kerry F Shea Claire M Wells Andrew P Garner Gareth E Jones ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. PLoS ONE |
| title | ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. |
| title_full | ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. |
| title_fullStr | ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. |
| title_full_unstemmed | ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. |
| title_short | ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. |
| title_sort | rock1 and limk2 interact in spread but not blebbing cancer cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0003398&type=printable |
| work_keys_str_mv | AT kerryfshea rock1andlimk2interactinspreadbutnotblebbingcancercells AT clairemwells rock1andlimk2interactinspreadbutnotblebbingcancercells AT andrewpgarner rock1andlimk2interactinspreadbutnotblebbingcancercells AT garethejones rock1andlimk2interactinspreadbutnotblebbingcancercells |