The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru.
<h4>Background</h4>The comparison of Mycobacterium tuberculosis bacterial genotypes with phenotypic, demographic, geospatial and clinical data improves our understanding of how strain lineage influences the development of drug-resistance and the spread of tuberculosis.<h4>Methods&l...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0126271 |
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| author | Louis Grandjean Tomotada Iwamoto Anna Lithgow Robert H Gilman Kentaro Arikawa Noriko Nakanishi Laura Martin Edith Castillo Valentina Alarcon Jorge Coronel Walter Solano Minoo Aminian Claudia Guezala Nalin Rastogi David Couvin Patricia Sheen Mirko Zimic David A J Moore |
| author_facet | Louis Grandjean Tomotada Iwamoto Anna Lithgow Robert H Gilman Kentaro Arikawa Noriko Nakanishi Laura Martin Edith Castillo Valentina Alarcon Jorge Coronel Walter Solano Minoo Aminian Claudia Guezala Nalin Rastogi David Couvin Patricia Sheen Mirko Zimic David A J Moore |
| author_sort | Louis Grandjean |
| collection | DOAJ |
| description | <h4>Background</h4>The comparison of Mycobacterium tuberculosis bacterial genotypes with phenotypic, demographic, geospatial and clinical data improves our understanding of how strain lineage influences the development of drug-resistance and the spread of tuberculosis.<h4>Methods</h4>To investigate the association of Mycobacterium tuberculosis bacterial genotype with drug-resistance. Drug susceptibility testing together with genotyping using both 15-loci MIRU-typing and spoligotyping, was performed on 2,139 culture positive isolates, each from a different patient in Lima, Peru. Demographic, geospatial and socio-economic data were collected using questionnaires, global positioning equipment and the latest national census.<h4>Results</h4>The Latin American Mediterranean (LAM) clade (OR 2.4, p<0.001) was significantly associated with drug-resistance and alone accounted for more than half of all drug resistance in the region. Previously treated patients, prisoners and genetically clustered cases were also significantly associated with drug-resistance (OR's 2.5, 2.4 and 1.8, p<0.001, p<0.05, p<0.001 respectively).<h4>Conclusions</h4>Tuberculosis disease caused by the LAM clade was more likely to be drug resistant independent of important clinical, genetic and socio-economic confounding factors. Explanations for this include; the preferential co-evolution of LAM strains in a Latin American population, a LAM strain bacterial genetic background that favors drug-resistance or the "founder effect" from pre-existing LAM strains disproportionately exposed to drugs. |
| format | Article |
| id | doaj-art-3a1ae4f1003944d188632b43a07f1f2e |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-3a1ae4f1003944d188632b43a07f1f2e2025-08-20T03:10:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012627110.1371/journal.pone.0126271The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru.Louis GrandjeanTomotada IwamotoAnna LithgowRobert H GilmanKentaro ArikawaNoriko NakanishiLaura MartinEdith CastilloValentina AlarconJorge CoronelWalter SolanoMinoo AminianClaudia GuezalaNalin RastogiDavid CouvinPatricia SheenMirko ZimicDavid A J Moore<h4>Background</h4>The comparison of Mycobacterium tuberculosis bacterial genotypes with phenotypic, demographic, geospatial and clinical data improves our understanding of how strain lineage influences the development of drug-resistance and the spread of tuberculosis.<h4>Methods</h4>To investigate the association of Mycobacterium tuberculosis bacterial genotype with drug-resistance. Drug susceptibility testing together with genotyping using both 15-loci MIRU-typing and spoligotyping, was performed on 2,139 culture positive isolates, each from a different patient in Lima, Peru. Demographic, geospatial and socio-economic data were collected using questionnaires, global positioning equipment and the latest national census.<h4>Results</h4>The Latin American Mediterranean (LAM) clade (OR 2.4, p<0.001) was significantly associated with drug-resistance and alone accounted for more than half of all drug resistance in the region. Previously treated patients, prisoners and genetically clustered cases were also significantly associated with drug-resistance (OR's 2.5, 2.4 and 1.8, p<0.001, p<0.05, p<0.001 respectively).<h4>Conclusions</h4>Tuberculosis disease caused by the LAM clade was more likely to be drug resistant independent of important clinical, genetic and socio-economic confounding factors. Explanations for this include; the preferential co-evolution of LAM strains in a Latin American population, a LAM strain bacterial genetic background that favors drug-resistance or the "founder effect" from pre-existing LAM strains disproportionately exposed to drugs.https://doi.org/10.1371/journal.pone.0126271 |
| spellingShingle | Louis Grandjean Tomotada Iwamoto Anna Lithgow Robert H Gilman Kentaro Arikawa Noriko Nakanishi Laura Martin Edith Castillo Valentina Alarcon Jorge Coronel Walter Solano Minoo Aminian Claudia Guezala Nalin Rastogi David Couvin Patricia Sheen Mirko Zimic David A J Moore The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru. PLoS ONE |
| title | The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru. |
| title_full | The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru. |
| title_fullStr | The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru. |
| title_full_unstemmed | The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru. |
| title_short | The Association between Mycobacterium Tuberculosis Genotype and Drug Resistance in Peru. |
| title_sort | association between mycobacterium tuberculosis genotype and drug resistance in peru |
| url | https://doi.org/10.1371/journal.pone.0126271 |
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