Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction
A simple and rapid capillary electrophoresis method with diode array detector was developed for determination of sarafloxacin and difloxacin in beef. In this study, the experimental parameters affecting the determination were systematically optimized, including wavelength, buffer system, pH and conc...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/1981436 |
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| author | Qian Wang Rui Zhao Yan Cui Cuicui Liu Liya Liang Shijie Yan |
| author_facet | Qian Wang Rui Zhao Yan Cui Cuicui Liu Liya Liang Shijie Yan |
| author_sort | Qian Wang |
| collection | DOAJ |
| description | A simple and rapid capillary electrophoresis method with diode array detector was developed for determination of sarafloxacin and difloxacin in beef. In this study, the experimental parameters affecting the determination were systematically optimized, including wavelength, buffer system, pH and concentration, and separation temperature and voltage. Under the optimal conditions, sarafloxacin and difloxacin could be quantified within 4 min using H3BO3/Na2B4O7 buffer (35 mmol/L, pH 8.8) as background electrolyte, 25 kV as separation voltage, and 22°C as the column temperature. The linear range of the method was 1–20 μg/mL with LOD 0.8 μg/mL for sarafloxacin and 0.5–20 μg/mL with LOD 0.3 μg/mL for difloxacin. The RSDs for the peak area of 8 μg/mL sarafloxacin were 4.8% (intraday) and 7.8% (interday), respectively. The proposed method has been applied to determine the residue of sarafloxacin and difloxacin in beef samples with the satisfactory recovery. |
| format | Article |
| id | doaj-art-3a0a26e931d04907a2e68c55b54a2da1 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-3a0a26e931d04907a2e68c55b54a2da12025-08-20T03:55:40ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/19814361981436Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase ExtractionQian Wang0Rui Zhao1Yan Cui2Cuicui Liu3Liya Liang4Shijie Yan5Department of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, ChinaDepartment of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, ChinaDepartment of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, ChinaDepartment of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, ChinaDepartment of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, ChinaDepartment of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, ChinaA simple and rapid capillary electrophoresis method with diode array detector was developed for determination of sarafloxacin and difloxacin in beef. In this study, the experimental parameters affecting the determination were systematically optimized, including wavelength, buffer system, pH and concentration, and separation temperature and voltage. Under the optimal conditions, sarafloxacin and difloxacin could be quantified within 4 min using H3BO3/Na2B4O7 buffer (35 mmol/L, pH 8.8) as background electrolyte, 25 kV as separation voltage, and 22°C as the column temperature. The linear range of the method was 1–20 μg/mL with LOD 0.8 μg/mL for sarafloxacin and 0.5–20 μg/mL with LOD 0.3 μg/mL for difloxacin. The RSDs for the peak area of 8 μg/mL sarafloxacin were 4.8% (intraday) and 7.8% (interday), respectively. The proposed method has been applied to determine the residue of sarafloxacin and difloxacin in beef samples with the satisfactory recovery.http://dx.doi.org/10.1155/2018/1981436 |
| spellingShingle | Qian Wang Rui Zhao Yan Cui Cuicui Liu Liya Liang Shijie Yan Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction Journal of Analytical Methods in Chemistry |
| title | Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction |
| title_full | Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction |
| title_fullStr | Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction |
| title_full_unstemmed | Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction |
| title_short | Rapid and Simple Determination of Sarafloxacin and Difloxacin in Beef by Capillary Electrophoresis Coupled with Solid-Phase Extraction |
| title_sort | rapid and simple determination of sarafloxacin and difloxacin in beef by capillary electrophoresis coupled with solid phase extraction |
| url | http://dx.doi.org/10.1155/2018/1981436 |
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