Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway
Transplantation of mesenchymal stem cells has been considered as an auspicious treatment for repairing nerve injuries. The rat adrenal pheochromocytoma cell line (PC12) is one of the traditional models for the study of neuronal differentiation and neuroregeneration in vitro. However, the effects of...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/2229477 |
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| author | Yong Xie Yangping Chen Yuyuan Zhu Xia Chen Tiecheng Lin Dan Zhou |
| author_facet | Yong Xie Yangping Chen Yuyuan Zhu Xia Chen Tiecheng Lin Dan Zhou |
| author_sort | Yong Xie |
| collection | DOAJ |
| description | Transplantation of mesenchymal stem cells has been considered as an auspicious treatment for repairing nerve injuries. The rat adrenal pheochromocytoma cell line (PC12) is one of the traditional models for the study of neuronal differentiation and neuroregeneration in vitro. However, the effects of adipose mesenchymal stem cell-derived exosomes (ADSC-exo) on PC12 cells remain unclear and to be elucidated. In our study, the effects of ADSC-exo on PC12 cells were investigated. ADSC-exo were isolated by ultracentrifugation and characterized by transmission electron microscopy, flow nanoanalysis, and western blot. The effects of ADSC-exo on PC12 cell proliferation, migration, apoptosis, and the protein levels were analyzed using CCK-8 assay and EdU incorporation assay, transwell migration assay and scratch wound assay, flow cytometry, and western blot, respectively. We successfully isolated and purified exosomes from ADSC supernatant and found that ADSC-exo treatment significantly promoted PC12 cell proliferation and migration, inhibited their apoptosis, and activated the PI3K/AKT pathway, while PI3K/AKT signaling repression using LY294002 exhibited the opposite effects. The results showed that ADSC-exo promoted proliferation and migration and inhibited apoptosis of PC12 through the activation of the PI3K/AKT pathway. Thus, the effect of ADSC-exo on PC12 cells may suggest ADSC-exo may be a promising therapeutic for nerve damage. |
| format | Article |
| id | doaj-art-39fbdd0e492b4244bc11e1120f8a6e7d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-39fbdd0e492b4244bc11e1120f8a6e7d2025-08-20T03:35:51ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/22294772229477Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT PathwayYong Xie0Yangping Chen1Yuyuan Zhu2Xia Chen3Tiecheng Lin4Dan Zhou5Department of Obstetrics and Gynecology, The First People’s Hospital of Foshan, Foshan 528000, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Foshan, Foshan 528000, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Foshan, Foshan 528000, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Foshan, Foshan 528000, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Foshan, Foshan 528000, ChinaDepartment of Galactophore Surgery, The First People’s Hospital of Foshan, Foshan 528000, ChinaTransplantation of mesenchymal stem cells has been considered as an auspicious treatment for repairing nerve injuries. The rat adrenal pheochromocytoma cell line (PC12) is one of the traditional models for the study of neuronal differentiation and neuroregeneration in vitro. However, the effects of adipose mesenchymal stem cell-derived exosomes (ADSC-exo) on PC12 cells remain unclear and to be elucidated. In our study, the effects of ADSC-exo on PC12 cells were investigated. ADSC-exo were isolated by ultracentrifugation and characterized by transmission electron microscopy, flow nanoanalysis, and western blot. The effects of ADSC-exo on PC12 cell proliferation, migration, apoptosis, and the protein levels were analyzed using CCK-8 assay and EdU incorporation assay, transwell migration assay and scratch wound assay, flow cytometry, and western blot, respectively. We successfully isolated and purified exosomes from ADSC supernatant and found that ADSC-exo treatment significantly promoted PC12 cell proliferation and migration, inhibited their apoptosis, and activated the PI3K/AKT pathway, while PI3K/AKT signaling repression using LY294002 exhibited the opposite effects. The results showed that ADSC-exo promoted proliferation and migration and inhibited apoptosis of PC12 through the activation of the PI3K/AKT pathway. Thus, the effect of ADSC-exo on PC12 cells may suggest ADSC-exo may be a promising therapeutic for nerve damage.http://dx.doi.org/10.1155/2021/2229477 |
| spellingShingle | Yong Xie Yangping Chen Yuyuan Zhu Xia Chen Tiecheng Lin Dan Zhou Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway Stem Cells International |
| title | Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway |
| title_full | Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway |
| title_fullStr | Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway |
| title_full_unstemmed | Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway |
| title_short | Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway |
| title_sort | adipose mesenchymal stem cell derived exosomes enhance pc12 cell function through the activation of the pi3k akt pathway |
| url | http://dx.doi.org/10.1155/2021/2229477 |
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