Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics
One of the primary opportunistic pathogens that can cause a wide range of diseases is <i>Pseudomonas aeruginosa</i>. This microorganism can become resistant to practically every antibacterial currently in use, including beta-lactam antibiotics. Its ability to proliferate as biofilm has b...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-08-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/13/9/800 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850258705382113280 |
|---|---|
| author | Guillermo Santamaría-Corral John Jairo Aguilera-Correa Jaime Esteban Meritxell García-Quintanilla |
| author_facet | Guillermo Santamaría-Corral John Jairo Aguilera-Correa Jaime Esteban Meritxell García-Quintanilla |
| author_sort | Guillermo Santamaría-Corral |
| collection | DOAJ |
| description | One of the primary opportunistic pathogens that can cause a wide range of diseases is <i>Pseudomonas aeruginosa</i>. This microorganism can become resistant to practically every antibacterial currently in use, including beta-lactam antibiotics. Its ability to proliferate as biofilm has been linked to, among other things, the failure of antimicrobial therapies. Due to a variety of virulence factors and host immune system modifications, <i>P. aeruginosa</i> is one of the most significant and common bacteria that colonize wounds and burns. A novel therapeutic option for treating these multidrug-resistant (MDR) bacterial infections is the combination of antibiotics and bacteriophages. This approach has been linked to improved biofilm penetration, a decreased selection of antibiotic and bacteriophage resistance, and an enhanced antibacterial impact. Combining the F1Pa bacteriophage and beta-lactam antibiotics reduced the viability of the mature biofilm of MDR <i>P. aeruginosa</i> strains and suppressed bacterial growth in vitro. F1Pa critically reduced the amount of biofilm that MDR <i>P. aeruginosa</i> clinical strains formed in the in vitro wound model. These findings highlight the bacteriophage F1Pa’s therapeutic potential as a prophylactic topical treatment against MDR pseudomonal infections in wounds and burns. |
| format | Article |
| id | doaj-art-39f407756f0347b9ae7f8f9a538fe16b |
| institution | OA Journals |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-39f407756f0347b9ae7f8f9a538fe16b2025-08-20T01:56:04ZengMDPI AGAntibiotics2079-63822024-08-0113980010.3390/antibiotics13090800Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam AntibioticsGuillermo Santamaría-Corral0John Jairo Aguilera-Correa1Jaime Esteban2Meritxell García-Quintanilla3Clinical Microbiology Department, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid, 28040 Madrid, SpainClinical Microbiology Department, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid, 28040 Madrid, SpainClinical Microbiology Department, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid, 28040 Madrid, SpainClinical Microbiology Department, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid, 28040 Madrid, SpainOne of the primary opportunistic pathogens that can cause a wide range of diseases is <i>Pseudomonas aeruginosa</i>. This microorganism can become resistant to practically every antibacterial currently in use, including beta-lactam antibiotics. Its ability to proliferate as biofilm has been linked to, among other things, the failure of antimicrobial therapies. Due to a variety of virulence factors and host immune system modifications, <i>P. aeruginosa</i> is one of the most significant and common bacteria that colonize wounds and burns. A novel therapeutic option for treating these multidrug-resistant (MDR) bacterial infections is the combination of antibiotics and bacteriophages. This approach has been linked to improved biofilm penetration, a decreased selection of antibiotic and bacteriophage resistance, and an enhanced antibacterial impact. Combining the F1Pa bacteriophage and beta-lactam antibiotics reduced the viability of the mature biofilm of MDR <i>P. aeruginosa</i> strains and suppressed bacterial growth in vitro. F1Pa critically reduced the amount of biofilm that MDR <i>P. aeruginosa</i> clinical strains formed in the in vitro wound model. These findings highlight the bacteriophage F1Pa’s therapeutic potential as a prophylactic topical treatment against MDR pseudomonal infections in wounds and burns.https://www.mdpi.com/2079-6382/13/9/800bacteriophage<i>Pseudomonas aeruginosa</i>multi-drug resistancebeta-lactamwound infection |
| spellingShingle | Guillermo Santamaría-Corral John Jairo Aguilera-Correa Jaime Esteban Meritxell García-Quintanilla Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics Antibiotics bacteriophage <i>Pseudomonas aeruginosa</i> multi-drug resistance beta-lactam wound infection |
| title | Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics |
| title_full | Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics |
| title_fullStr | Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics |
| title_full_unstemmed | Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics |
| title_short | Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics |
| title_sort | bacteriophage therapy on an in vitro wound model and synergistic effects in combination with beta lactam antibiotics |
| topic | bacteriophage <i>Pseudomonas aeruginosa</i> multi-drug resistance beta-lactam wound infection |
| url | https://www.mdpi.com/2079-6382/13/9/800 |
| work_keys_str_mv | AT guillermosantamariacorral bacteriophagetherapyonaninvitrowoundmodelandsynergisticeffectsincombinationwithbetalactamantibiotics AT johnjairoaguileracorrea bacteriophagetherapyonaninvitrowoundmodelandsynergisticeffectsincombinationwithbetalactamantibiotics AT jaimeesteban bacteriophagetherapyonaninvitrowoundmodelandsynergisticeffectsincombinationwithbetalactamantibiotics AT meritxellgarciaquintanilla bacteriophagetherapyonaninvitrowoundmodelandsynergisticeffectsincombinationwithbetalactamantibiotics |