Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible

Lateral or vertical bone augmentation has always been a challenge, since the site is exposed to constant pressure from the soft tissue, and blood supply only exists from the donor site. Although, for such clinical cases, onlay grafting with autogenous bone is commonly selected, the invasiveness of t...

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Main Authors: Charles Marin, Ryo Jimbo, Fabio Cesar Lorenzoni, Lukasz Witek, Hellen Teixeira, Estevam Bonfante, Jose Gil, Rodrigo Granato, Nick Tovar, Paulo G. Coelho
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2013/296391
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author Charles Marin
Ryo Jimbo
Fabio Cesar Lorenzoni
Lukasz Witek
Hellen Teixeira
Estevam Bonfante
Jose Gil
Rodrigo Granato
Nick Tovar
Paulo G. Coelho
author_facet Charles Marin
Ryo Jimbo
Fabio Cesar Lorenzoni
Lukasz Witek
Hellen Teixeira
Estevam Bonfante
Jose Gil
Rodrigo Granato
Nick Tovar
Paulo G. Coelho
author_sort Charles Marin
collection DOAJ
description Lateral or vertical bone augmentation has always been a challenge, since the site is exposed to constant pressure from the soft tissue, and blood supply only exists from the donor site. Although, for such clinical cases, onlay grafting with autogenous bone is commonly selected, the invasiveness of the secondary surgical site and the relatively fast resorption rate have been reported as a drawback, which motivated the investigation of alternative approaches. This study evaluated the bone-forming capability of a novel nanoHA alloplast infused with collagen graft material made from biodegradable polylactic acid/polyglycolic acid versus a control graft material with the same synthesized alloplast without the nanoHA component and collagen infiltration. The status of newly formed bone and the resorption of the graft material were evaluated at 6 weeks in vivo histologically and three dimensionally by means of 3D microcomputed tomography. The histologic observation showed that newly formed bone ingrowth and internal resorption of the block were observed for the experimental blocks, whereas for the control blocks less bone ingrowth occurred along with lower resorption rate of the block material. The three-dimensional observation indicated that the experimental block maintained the external geometry, but at the same time successfully altered the graft material into bone. It is suggested that the combination of numerous factors contributed to the bone ingrowth and the novel development could be an alternative bone grafting choice.
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spelling doaj-art-39e6c98c2e3b48edbbdc49a90b91762a2025-08-20T03:35:50ZengWileyInternational Journal of Dentistry1687-87281687-87362013-01-01201310.1155/2013/296391296391Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep MandibleCharles Marin0Ryo Jimbo1Fabio Cesar Lorenzoni2Lukasz Witek3Hellen Teixeira4Estevam Bonfante5Jose Gil6Rodrigo Granato7Nick Tovar8Paulo G. Coelho9Department of Dentistry, UNIGRANRIO, 25071-202 Duque de Caxias, RJ, BrazilDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDepartment of Biomaterials and Biomimetics, New York University, New York, NY 10010, USASchool of Chemical Engineering, Oklahoma State University, Stillwater, OK 74075, USADepartment of Biomaterials and Biomimetics, New York University, New York, NY 10010, USADepartment of Prosthodontics, Integrated Center for Research, Bauru School of Dentistry, University of São Paulo, 17012-901 Bauru, SP, BrazilPostgraduate Program in Dentistry, UNIGRANRIO University-School of Health Sciences, 25071-202 Duque de Caxias, RJ, BrazilPostgraduate Program in Dentistry, UNIGRANRIO University-School of Health Sciences, 25071-202 Duque de Caxias, RJ, BrazilDepartment of Biomaterials and Biomimetics, New York University, New York, NY 10010, USADepartment of Biomaterials and Biomimetics, New York University, New York, NY 10010, USALateral or vertical bone augmentation has always been a challenge, since the site is exposed to constant pressure from the soft tissue, and blood supply only exists from the donor site. Although, for such clinical cases, onlay grafting with autogenous bone is commonly selected, the invasiveness of the secondary surgical site and the relatively fast resorption rate have been reported as a drawback, which motivated the investigation of alternative approaches. This study evaluated the bone-forming capability of a novel nanoHA alloplast infused with collagen graft material made from biodegradable polylactic acid/polyglycolic acid versus a control graft material with the same synthesized alloplast without the nanoHA component and collagen infiltration. The status of newly formed bone and the resorption of the graft material were evaluated at 6 weeks in vivo histologically and three dimensionally by means of 3D microcomputed tomography. The histologic observation showed that newly formed bone ingrowth and internal resorption of the block were observed for the experimental blocks, whereas for the control blocks less bone ingrowth occurred along with lower resorption rate of the block material. The three-dimensional observation indicated that the experimental block maintained the external geometry, but at the same time successfully altered the graft material into bone. It is suggested that the combination of numerous factors contributed to the bone ingrowth and the novel development could be an alternative bone grafting choice.http://dx.doi.org/10.1155/2013/296391
spellingShingle Charles Marin
Ryo Jimbo
Fabio Cesar Lorenzoni
Lukasz Witek
Hellen Teixeira
Estevam Bonfante
Jose Gil
Rodrigo Granato
Nick Tovar
Paulo G. Coelho
Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible
International Journal of Dentistry
title Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible
title_full Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible
title_fullStr Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible
title_full_unstemmed Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible
title_short Bone-Forming Capabilities of a Newly Developed NanoHA Composite Alloplast Infused with Collagen: A Pilot Study in the Sheep Mandible
title_sort bone forming capabilities of a newly developed nanoha composite alloplast infused with collagen a pilot study in the sheep mandible
url http://dx.doi.org/10.1155/2013/296391
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