In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis
AIM: This study aims to assess the in vitro anti-Leishmania activity of Dracocephalum lindbergii (D. lindbergii) and its essential oils. BACKGROUND: The plant, belonging to the Lamiaceae family, has a history of traditional medicinal use. Leishmaniasis, a vector-borne disease caused by protozoan par...
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524003151 |
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| author | Faham Khamesipour Ali Khamesipour Seyed Hossein Hejazi Mustafa Ghanadian |
| author_facet | Faham Khamesipour Ali Khamesipour Seyed Hossein Hejazi Mustafa Ghanadian |
| author_sort | Faham Khamesipour |
| collection | DOAJ |
| description | AIM: This study aims to assess the in vitro anti-Leishmania activity of Dracocephalum lindbergii (D. lindbergii) and its essential oils. BACKGROUND: The plant, belonging to the Lamiaceae family, has a history of traditional medicinal use. Leishmaniasis, a vector-borne disease caused by protozoan parasites of the genus Leishmania, poses significant health risks. METHODS: Aerial parts of D. lindbergii were collected from Iran, and essential components were extracted using a series of solvents. Gas chromatography coupled with mass spectrometry was utilized for component analysis. The impact of D. lindbergii fractions and essential oil on Leishmania major promastigotes, amastigotes, and macrophages was assessed using the MTT method for cell viability and flow cytometry for apoptosis. Real-time PCR quantified cytokine expression on murine macrophages. RESULTS: Results show the essential oil's potent anti-Leishmania activity against amastigotes after 24 hours. Fraction F1 was most effective against promastigotes, while F3 showed high activity against macrophages with a favorable selectivity index. Time-dependent variations in activity were observed. Cellular morphology changes included shrinkage and cytoplasmic condensation after treatment. Fraction F4 exhibited notable antioxidant properties. The results also indicated time-dependent variations in the anti-Leishmania activity of the essential oil and the fractions. CONCLUSIONS: D. lindbergii and its essential oils hold promise as anti-Leishmania agents, with the essential oil and F4 fraction being potential candidates for drug development. Further research and clinical trials are necessary to explore their therapeutic potential fully. |
| format | Article |
| id | doaj-art-39e56520e29e447ca211980f3b82b868 |
| institution | DOAJ |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-39e56520e29e447ca211980f3b82b8682025-08-20T02:57:22ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-01394310.1016/j.jgar.2024.10.138In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysisFaham Khamesipour0Ali Khamesipour1Seyed Hossein Hejazi2Mustafa Ghanadian3Halal Research Center of the Islamic Republic of Iran (IRI), Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, IranCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IranAIM: This study aims to assess the in vitro anti-Leishmania activity of Dracocephalum lindbergii (D. lindbergii) and its essential oils. BACKGROUND: The plant, belonging to the Lamiaceae family, has a history of traditional medicinal use. Leishmaniasis, a vector-borne disease caused by protozoan parasites of the genus Leishmania, poses significant health risks. METHODS: Aerial parts of D. lindbergii were collected from Iran, and essential components were extracted using a series of solvents. Gas chromatography coupled with mass spectrometry was utilized for component analysis. The impact of D. lindbergii fractions and essential oil on Leishmania major promastigotes, amastigotes, and macrophages was assessed using the MTT method for cell viability and flow cytometry for apoptosis. Real-time PCR quantified cytokine expression on murine macrophages. RESULTS: Results show the essential oil's potent anti-Leishmania activity against amastigotes after 24 hours. Fraction F1 was most effective against promastigotes, while F3 showed high activity against macrophages with a favorable selectivity index. Time-dependent variations in activity were observed. Cellular morphology changes included shrinkage and cytoplasmic condensation after treatment. Fraction F4 exhibited notable antioxidant properties. The results also indicated time-dependent variations in the anti-Leishmania activity of the essential oil and the fractions. CONCLUSIONS: D. lindbergii and its essential oils hold promise as anti-Leishmania agents, with the essential oil and F4 fraction being potential candidates for drug development. Further research and clinical trials are necessary to explore their therapeutic potential fully.http://www.sciencedirect.com/science/article/pii/S2213716524003151Dracocephalum lindbergiiApoptosisTraditionnel medicineLeishmania major and promastigotes |
| spellingShingle | Faham Khamesipour Ali Khamesipour Seyed Hossein Hejazi Mustafa Ghanadian In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis Journal of Global Antimicrobial Resistance Dracocephalum lindbergii Apoptosis Traditionnel medicine Leishmania major and promastigotes |
| title | In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis |
| title_full | In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis |
| title_fullStr | In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis |
| title_full_unstemmed | In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis |
| title_short | In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis |
| title_sort | in vitro evaluation of dracocephalum lindbergii on growth and apoptosis induction against leishmania major and cytokine analysis |
| topic | Dracocephalum lindbergii Apoptosis Traditionnel medicine Leishmania major and promastigotes |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524003151 |
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