Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production
Abstract Collagen, recognized as the primary structural component of human skin, is essential for preserving dermal integrity and function. Its progressive depletion has been closely associated with structural deterioration of the dermis and the visible signs of skin aging. Among current therapeutic...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-07-01
|
| Series: | Collagen and Leather |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42825-025-00203-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849767476750974976 |
|---|---|
| author | Chen Hu Kang Li Li Li Xinyue Zhang Tongjie Xu Liguo Zhang Jie Liang Yafang Chen Yujiang Fan |
| author_facet | Chen Hu Kang Li Li Li Xinyue Zhang Tongjie Xu Liguo Zhang Jie Liang Yafang Chen Yujiang Fan |
| author_sort | Chen Hu |
| collection | DOAJ |
| description | Abstract Collagen, recognized as the primary structural component of human skin, is essential for preserving dermal integrity and function. Its progressive depletion has been closely associated with structural deterioration of the dermis and the visible signs of skin aging. Among current therapeutic strategies, the injection of exogenous collagen has been established as an effective method for alleviating aging-related skin changes. In the present study, a comprehensive evaluation was conducted to assess the injectability, cellular interactions, and photoaging repair efficacy of recombinant human collagen type III (RHC). The RHC solution was found to demonstrate favorable injectability and support the adhesion and chemotactic behavior of L929 cells, while also upregulating the expression of type I and type III collagen. In co-culture systems with lipopolysaccharide-stimulated macrophages, RHC treatment suppressed macrophage proliferation and reduced the production of proinflammatory cytokines, suggesting notable immunomodulatory properties. Upon intradermal injection of RHC into photoaged rat skin, an increased density of dermal collagen fibers was observed, accompanied by a more organized and uniform fiber arrangement. Additionally, hydroxyproline content and the expressions of collagen I and III were markedly elevated in the RHC group compared with the control and hyaluronic acid groups. Collectively, these findings suggest that RHC holds considerable promise as a therapeutic agent for both medical and cosmetic purposes targeting the restoration and maintenance of youthful skin characteristics. Graphical Abstract |
| format | Article |
| id | doaj-art-39e553f8524147f5acaa137703c2d9ce |
| institution | DOAJ |
| issn | 2097-1419 2731-6998 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Collagen and Leather |
| spelling | doaj-art-39e553f8524147f5acaa137703c2d9ce2025-08-20T03:04:11ZengSpringerOpenCollagen and Leather2097-14192731-69982025-07-017111810.1186/s42825-025-00203-yRecombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen productionChen Hu0Kang Li1Li Li2Xinyue Zhang3Tongjie Xu4Liguo Zhang5Jie Liang6Yafang Chen7Yujiang Fan8National Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityInstitute of Clinical Pathology, West China Hospital of Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityHarbin Fuerjia Technology Co., Ltd.Harbin Fuerjia Technology Co., Ltd.National Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityNational Engineering Research Center for Biomaterials, Sichuan UniversityAbstract Collagen, recognized as the primary structural component of human skin, is essential for preserving dermal integrity and function. Its progressive depletion has been closely associated with structural deterioration of the dermis and the visible signs of skin aging. Among current therapeutic strategies, the injection of exogenous collagen has been established as an effective method for alleviating aging-related skin changes. In the present study, a comprehensive evaluation was conducted to assess the injectability, cellular interactions, and photoaging repair efficacy of recombinant human collagen type III (RHC). The RHC solution was found to demonstrate favorable injectability and support the adhesion and chemotactic behavior of L929 cells, while also upregulating the expression of type I and type III collagen. In co-culture systems with lipopolysaccharide-stimulated macrophages, RHC treatment suppressed macrophage proliferation and reduced the production of proinflammatory cytokines, suggesting notable immunomodulatory properties. Upon intradermal injection of RHC into photoaged rat skin, an increased density of dermal collagen fibers was observed, accompanied by a more organized and uniform fiber arrangement. Additionally, hydroxyproline content and the expressions of collagen I and III were markedly elevated in the RHC group compared with the control and hyaluronic acid groups. Collectively, these findings suggest that RHC holds considerable promise as a therapeutic agent for both medical and cosmetic purposes targeting the restoration and maintenance of youthful skin characteristics. Graphical Abstracthttps://doi.org/10.1186/s42825-025-00203-yRecombinant human collagen type III injectionImmunomodulatory potentialInducing collagen regenerationAmeliorating skin photoaging |
| spellingShingle | Chen Hu Kang Li Li Li Xinyue Zhang Tongjie Xu Liguo Zhang Jie Liang Yafang Chen Yujiang Fan Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production Collagen and Leather Recombinant human collagen type III injection Immunomodulatory potential Inducing collagen regeneration Ameliorating skin photoaging |
| title | Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production |
| title_full | Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production |
| title_fullStr | Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production |
| title_full_unstemmed | Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production |
| title_short | Recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production |
| title_sort | recombinant human collagen injection ameliorates skin photoaging via immunoregulation and collagen production |
| topic | Recombinant human collagen type III injection Immunomodulatory potential Inducing collagen regeneration Ameliorating skin photoaging |
| url | https://doi.org/10.1186/s42825-025-00203-y |
| work_keys_str_mv | AT chenhu recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT kangli recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT lili recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT xinyuezhang recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT tongjiexu recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT liguozhang recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT jieliang recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT yafangchen recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction AT yujiangfan recombinanthumancollageninjectionamelioratesskinphotoagingviaimmunoregulationandcollagenproduction |