The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet
Fluorescent iron nanoclusters are emerging fluorescent nanomaterials. Herein, we synthesized hemoglobin-coated iron nanoclusters (Hb−Fe NCs) with a significant fluorescence emission peak at 615 nm and investigated the inner-filter effect of fluorescence induced by a manganese dioxide nanosheet (MnO&...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/1424-8220/25/2/585 |
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| author | Liang Zhao Xinyue Liu Xinwen Zhang Siyu Liu Jiazhen Wu |
| author_facet | Liang Zhao Xinyue Liu Xinwen Zhang Siyu Liu Jiazhen Wu |
| author_sort | Liang Zhao |
| collection | DOAJ |
| description | Fluorescent iron nanoclusters are emerging fluorescent nanomaterials. Herein, we synthesized hemoglobin-coated iron nanoclusters (Hb−Fe NCs) with a significant fluorescence emission peak at 615 nm and investigated the inner-filter effect of fluorescence induced by a manganese dioxide nanosheet (MnO<sub>2</sub> NS). The fluorescence quenching of Hb−Fe NCs by a MnO<sub>2</sub> NS can be significantly reversed by the addition of ascorbic acid. On the basis of fluorescent recovery by ascorbic acid, we proposed a system that consisted of Hb−Fe NCs, a MnO<sub>2</sub> NS and ascorbate phosphate, and the proposed system was successfully used for alkaline phosphatase (ALP) detection in the range of 0–20 μg/mL based on the significant fluorescence recovery achieved. |
| format | Article |
| id | doaj-art-39e3b827de91487c81ab0fa405e6ad18 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj-art-39e3b827de91487c81ab0fa405e6ad182025-01-24T13:49:26ZengMDPI AGSensors1424-82202025-01-0125258510.3390/s25020585The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide NanosheetLiang Zhao0Xinyue Liu1Xinwen Zhang2Siyu Liu3Jiazhen Wu4College of Life and Health Sciences, Northeastern University, Shenyang 110169, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang 110169, ChinaCollege of Medicine and Biological Information Engineering, Northeastern University, Shenyang 110057, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang 110169, ChinaDepartment of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaFluorescent iron nanoclusters are emerging fluorescent nanomaterials. Herein, we synthesized hemoglobin-coated iron nanoclusters (Hb−Fe NCs) with a significant fluorescence emission peak at 615 nm and investigated the inner-filter effect of fluorescence induced by a manganese dioxide nanosheet (MnO<sub>2</sub> NS). The fluorescence quenching of Hb−Fe NCs by a MnO<sub>2</sub> NS can be significantly reversed by the addition of ascorbic acid. On the basis of fluorescent recovery by ascorbic acid, we proposed a system that consisted of Hb−Fe NCs, a MnO<sub>2</sub> NS and ascorbate phosphate, and the proposed system was successfully used for alkaline phosphatase (ALP) detection in the range of 0–20 μg/mL based on the significant fluorescence recovery achieved.https://www.mdpi.com/1424-8220/25/2/585fluorescencedetectionALPiron nanoclusterMnO<sub>2</sub> NS |
| spellingShingle | Liang Zhao Xinyue Liu Xinwen Zhang Siyu Liu Jiazhen Wu The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet Sensors fluorescence detection ALP iron nanocluster MnO<sub>2</sub> NS |
| title | The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet |
| title_full | The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet |
| title_fullStr | The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet |
| title_full_unstemmed | The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet |
| title_short | The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet |
| title_sort | fluorescent detection of alkaline phosphatase based on iron nanoclusters and a manganese dioxide nanosheet |
| topic | fluorescence detection ALP iron nanocluster MnO<sub>2</sub> NS |
| url | https://www.mdpi.com/1424-8220/25/2/585 |
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