Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology
Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing tec...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124002782 |
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| author | Tingyu Gong Dandan Liu Xiaochen Wang Danni Zhou Ling Tang Hao Wang Jun Su Ping Liang |
| author_facet | Tingyu Gong Dandan Liu Xiaochen Wang Danni Zhou Ling Tang Hao Wang Jun Su Ping Liang |
| author_sort | Tingyu Gong |
| collection | DOAJ |
| description | Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype. |
| format | Article |
| id | doaj-art-39dd69a5ca404c95980a93f87cd26f90 |
| institution | OA Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-39dd69a5ca404c95980a93f87cd26f902025-08-20T02:38:17ZengElsevierStem Cell Research1873-50612024-12-018110358010.1016/j.scr.2024.103580Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technologyTingyu Gong0Dandan Liu1Xiaochen Wang2Danni Zhou3Ling Tang4Hao Wang5Jun Su6Ping Liang7Shulan International Medical College, Zhejiang Shuren University, Hangzhou, 310015, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China; Corresponding authors at: Key Laboratory of combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China; Corresponding authors at: Key Laboratory of combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype.http://www.sciencedirect.com/science/article/pii/S1873506124002782 |
| spellingShingle | Tingyu Gong Dandan Liu Xiaochen Wang Danni Zhou Ling Tang Hao Wang Jun Su Ping Liang Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology Stem Cell Research |
| title | Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology |
| title_full | Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology |
| title_fullStr | Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology |
| title_full_unstemmed | Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology |
| title_short | Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology |
| title_sort | establishment of a cib1 knockout human pluripotent stem cell line via crispr cas9 genome editing technology |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124002782 |
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