Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts

Nitrate and nitrite residues in food significantly impact human health. This paper developed a one-pot ultrasound-assisted cloud point extraction method for spectrophotometric nitrate and nitrite determination, utilizing an eco-friendly natural reagent consisting of Careya arborea Roxb. leaf extract...

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Main Authors: Sam-ang Supharoek, Chang Young Lee, Sasalux Kaewbutra, Sucha Jumpanon, Waraporn Threeprom, Kraingkrai Ponhong
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525002287
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author Sam-ang Supharoek
Chang Young Lee
Sasalux Kaewbutra
Sucha Jumpanon
Waraporn Threeprom
Kraingkrai Ponhong
author_facet Sam-ang Supharoek
Chang Young Lee
Sasalux Kaewbutra
Sucha Jumpanon
Waraporn Threeprom
Kraingkrai Ponhong
author_sort Sam-ang Supharoek
collection DOAJ
description Nitrate and nitrite residues in food significantly impact human health. This paper developed a one-pot ultrasound-assisted cloud point extraction method for spectrophotometric nitrate and nitrite determination, utilizing an eco-friendly natural reagent consisting of Careya arborea Roxb. leaf extract as an alternative to N-1-naphthyl ethylenediamine. In the proposed reaction, nitrate was reduced to nitrite utilizing vanadium (III) chloride. The nitrite then reacted with sulfanilamide and the natural reagent to yield a yellow product which was subsequently preconcentrated using Triton X-114. Nitrate reduction, azo-dye formation, and cloud point preconcentration were performed concurrently using a one-pot ultrasound-assisted approach. This method exhibited a good linear range with limits of detection for nitrite and nitrate 0.01 and 0.05 mg L−1, respectively. Our suggested approach effectively determined nitrate and nitrite in sausage samples, obtaining desirable recovery ranging from 90 % to 110 % and exhibiting strong agreement with Griess's method.
format Article
id doaj-art-39dcbf47e2dd4d9fb7b2cc8fdf8cd738
institution OA Journals
issn 2590-1575
language English
publishDate 2025-04-01
publisher Elsevier
record_format Article
series Food Chemistry: X
spelling doaj-art-39dcbf47e2dd4d9fb7b2cc8fdf8cd7382025-08-20T01:50:01ZengElsevierFood Chemistry: X2590-15752025-04-012710238110.1016/j.fochx.2025.102381Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extractsSam-ang Supharoek0Chang Young Lee1Sasalux Kaewbutra2Sucha Jumpanon3Waraporn Threeprom4Kraingkrai Ponhong5Department of Medical Science, Mahidol University, Amnatcharoen Campus, Amnat Charoen 37000, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Corresponding author at: Department of Medical Science, Mahidol University, Amnatcharoen Campus, Amnat Charoen 37000, Thailand.School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of KoreaDepartment of Medical Science, Mahidol University, Amnatcharoen Campus, Amnat Charoen 37000, Thailand; Unit of Water and Food Analysis, Department of Medical Science, Amnatcharoen Campus, Mahidol University, ThailandDepartment of Medical Science, Mahidol University, Amnatcharoen Campus, Amnat Charoen 37000, Thailand; Unit of Water and Food Analysis, Department of Medical Science, Amnatcharoen Campus, Mahidol University, ThailandSchool of Interdisciplinary Studies (Kanchanaburi Campus), Mahidol University, Kanchanaburi 71150, ThailandCreative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand; Multidisciplinary Research Unit of Pure and Applied Chemistry (MRUPAC), Department of Chemistry and Center of Excellent for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandNitrate and nitrite residues in food significantly impact human health. This paper developed a one-pot ultrasound-assisted cloud point extraction method for spectrophotometric nitrate and nitrite determination, utilizing an eco-friendly natural reagent consisting of Careya arborea Roxb. leaf extract as an alternative to N-1-naphthyl ethylenediamine. In the proposed reaction, nitrate was reduced to nitrite utilizing vanadium (III) chloride. The nitrite then reacted with sulfanilamide and the natural reagent to yield a yellow product which was subsequently preconcentrated using Triton X-114. Nitrate reduction, azo-dye formation, and cloud point preconcentration were performed concurrently using a one-pot ultrasound-assisted approach. This method exhibited a good linear range with limits of detection for nitrite and nitrate 0.01 and 0.05 mg L−1, respectively. Our suggested approach effectively determined nitrate and nitrite in sausage samples, obtaining desirable recovery ranging from 90 % to 110 % and exhibiting strong agreement with Griess's method.http://www.sciencedirect.com/science/article/pii/S2590157525002287Careya arborea Roxb.Cloud point extractionFood preservationNatural reagentNitrate and nitriteUltrasound-assisted one-pot
spellingShingle Sam-ang Supharoek
Chang Young Lee
Sasalux Kaewbutra
Sucha Jumpanon
Waraporn Threeprom
Kraingkrai Ponhong
Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts
Food Chemistry: X
Careya arborea Roxb.
Cloud point extraction
Food preservation
Natural reagent
Nitrate and nitrite
Ultrasound-assisted one-pot
title Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts
title_full Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts
title_fullStr Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts
title_full_unstemmed Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts
title_short Ultrasound-assisted one-pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using Careya arborea Roxb. leaf extracts
title_sort ultrasound assisted one pot cloud point extraction for spectrophotometric nitrate and nitrite determination in sausage products using careya arborea roxb leaf extracts
topic Careya arborea Roxb.
Cloud point extraction
Food preservation
Natural reagent
Nitrate and nitrite
Ultrasound-assisted one-pot
url http://www.sciencedirect.com/science/article/pii/S2590157525002287
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