Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein
Abstract The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, has evolved resistance to nearly every management tactic utilized in the field. This study investigated the resistance mechanisms in a WCR strain resistant to the Bacillus thuringiensis (Bt) protein eCry3.1Ab using dsR...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83135-7 |
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| author | Adriano E. Pereira Kyle J. Paddock Jacob A. Corcoran Zixiao Zhao Michelle L. J. Gregory Thomas A. Coudron Bruce E. Hibbard Kent S. Shelby Man P. Huynh |
| author_facet | Adriano E. Pereira Kyle J. Paddock Jacob A. Corcoran Zixiao Zhao Michelle L. J. Gregory Thomas A. Coudron Bruce E. Hibbard Kent S. Shelby Man P. Huynh |
| author_sort | Adriano E. Pereira |
| collection | DOAJ |
| description | Abstract The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, has evolved resistance to nearly every management tactic utilized in the field. This study investigated the resistance mechanisms in a WCR strain resistant to the Bacillus thuringiensis (Bt) protein eCry3.1Ab using dsRNA to knockdown WCR midgut genes previously documented to be associated with the resistance. ATP-binding cassette transporter (ABCC4), aminopeptidase-N, cadherin, and cathepsin-B were previously found to be differentially expressed in eCry3.1Ab-resistant WCR larvae when compared to susceptible larvae after feeding on maize expressing eCry3.1Ab and its near-isoline. Here we compared the susceptibility of resistant and susceptible WCR larvae to eCry3.1Ab protein in presence or absence of dsRNA targeting the above genes using 10-day diet overlay toxicity assays. Combining ABCC4 dsRNA with eCry3.1Ab protein increased susceptibility to Bt protein in WCR-resistant larvae, but the other three genes had no such effect. Among 65 ABC transport genes identified, several were expressed differently in resistant or susceptible WCR larvae, fed on eCry3.1Ab-expressing maize versus its isoline, that may be involved in Bt resistance. Our findings provide strong evidence that ABCC4 is indirectly involved in WCR resistance to eCry3.1Ab protein by enhancing the effects of Bt-induced toxicity. |
| format | Article |
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| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-39d48015cfa842e2b274bf3fb69e69a02025-08-20T02:39:34ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-83135-7Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab proteinAdriano E. Pereira0Kyle J. Paddock1Jacob A. Corcoran2Zixiao Zhao3Michelle L. J. Gregory4Thomas A. Coudron5Bruce E. Hibbard6Kent S. Shelby7Man P. Huynh8Division of Plant Science and Technology, University of MissouriBiological Control of Insects Research Laboratory, USDA-ARSBiological Control of Insects Research Laboratory, USDA-ARSDivision of Plant Science and Technology, University of MissouriBiological Control of Insects Research Laboratory, USDA-ARSBiological Control of Insects Research Laboratory, USDA-ARSBiological Control of Insects Research Laboratory, USDA-ARSBiological Control of Insects Research Laboratory, USDA-ARSDivision of Plant Science and Technology, University of MissouriAbstract The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, has evolved resistance to nearly every management tactic utilized in the field. This study investigated the resistance mechanisms in a WCR strain resistant to the Bacillus thuringiensis (Bt) protein eCry3.1Ab using dsRNA to knockdown WCR midgut genes previously documented to be associated with the resistance. ATP-binding cassette transporter (ABCC4), aminopeptidase-N, cadherin, and cathepsin-B were previously found to be differentially expressed in eCry3.1Ab-resistant WCR larvae when compared to susceptible larvae after feeding on maize expressing eCry3.1Ab and its near-isoline. Here we compared the susceptibility of resistant and susceptible WCR larvae to eCry3.1Ab protein in presence or absence of dsRNA targeting the above genes using 10-day diet overlay toxicity assays. Combining ABCC4 dsRNA with eCry3.1Ab protein increased susceptibility to Bt protein in WCR-resistant larvae, but the other three genes had no such effect. Among 65 ABC transport genes identified, several were expressed differently in resistant or susceptible WCR larvae, fed on eCry3.1Ab-expressing maize versus its isoline, that may be involved in Bt resistance. Our findings provide strong evidence that ABCC4 is indirectly involved in WCR resistance to eCry3.1Ab protein by enhancing the effects of Bt-induced toxicity.https://doi.org/10.1038/s41598-024-83135-7Diabrotica virgifera virgiferaBacillus thuringiensisCorn rootwormResistance mechanismsRNAiInsect resistance management |
| spellingShingle | Adriano E. Pereira Kyle J. Paddock Jacob A. Corcoran Zixiao Zhao Michelle L. J. Gregory Thomas A. Coudron Bruce E. Hibbard Kent S. Shelby Man P. Huynh Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein Scientific Reports Diabrotica virgifera virgifera Bacillus thuringiensis Corn rootworm Resistance mechanisms RNAi Insect resistance management |
| title | Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein |
| title_full | Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein |
| title_fullStr | Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein |
| title_full_unstemmed | Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein |
| title_short | Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein |
| title_sort | knockdown of an atp binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to ecry3 1ab protein |
| topic | Diabrotica virgifera virgifera Bacillus thuringiensis Corn rootworm Resistance mechanisms RNAi Insect resistance management |
| url | https://doi.org/10.1038/s41598-024-83135-7 |
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