Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle
Abstract Despite indisputable benefits of different exercise modes, the molecular underpinnings of their divergent responses remain unclear. We investigate post-translational modifications in human skeletal muscle following 12 weeks of high-intensity aerobic interval or resistance exercise training....
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60049-0 |
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| author | Mark W. Pataky Carrie J. Heppelmann Kyle J. Sevits Aneesh K. Asokan Arathi Prabha Kumar Katherine A. Klaus Surendra Dasari Hawley E. Kunz Matthew D. Strub Matthew M. Robinson Joshua J. Coon Ian R. Lanza Christopher M. Adams K. Sreekumaran Nair |
| author_facet | Mark W. Pataky Carrie J. Heppelmann Kyle J. Sevits Aneesh K. Asokan Arathi Prabha Kumar Katherine A. Klaus Surendra Dasari Hawley E. Kunz Matthew D. Strub Matthew M. Robinson Joshua J. Coon Ian R. Lanza Christopher M. Adams K. Sreekumaran Nair |
| author_sort | Mark W. Pataky |
| collection | DOAJ |
| description | Abstract Despite indisputable benefits of different exercise modes, the molecular underpinnings of their divergent responses remain unclear. We investigate post-translational modifications in human skeletal muscle following 12 weeks of high-intensity aerobic interval or resistance exercise training. High-intensity aerobic training induces acetylproteome modifications including several mitochondrial proteins, indicating post-translational regulation of energetics machinery, whereas resistance exercise training regulates phosphoproteomic modifications of contractile/cytoskeletal machinery, consistent with greater strength. Furthermore, despite similar transcriptional responses to a single acute bout of aerobic and resistance exercise, more robust phosphoproteomic and metabolomic responses occur with acute aerobic exercise, including phosphorylation of structural/contractile and membrane transport machinery, and the nascent polypeptide-associated complex-α, a regulator of protein translation. Together, our findings provide new insight on the intricate phosphoproteomic and acetylproteomic modifications in muscle that potentially explain physiological responses to different modes of chronic and acute exercise. This study is registered with ClinicalTrials.gov, numbers NCT01477164 and NCT04158375. |
| format | Article |
| id | doaj-art-39d2700ebf0c4a5887d7d27f6d06f9f5 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-39d2700ebf0c4a5887d7d27f6d06f9f52025-08-20T03:37:37ZengNature PortfolioNature Communications2041-17232025-07-0116111910.1038/s41467-025-60049-0Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscleMark W. Pataky0Carrie J. Heppelmann1Kyle J. Sevits2Aneesh K. Asokan3Arathi Prabha Kumar4Katherine A. Klaus5Surendra Dasari6Hawley E. Kunz7Matthew D. Strub8Matthew M. Robinson9Joshua J. Coon10Ian R. Lanza11Christopher M. Adams12K. Sreekumaran Nair13Division of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDepartment of Quantitative Health Sciences, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicSchool of Exercise, Sport, and Health Sciences, College of Health, Oregon State UniversityDepartment of Chemistry, University of Wisconsin-MadisonDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicDivision of Endocrinology and Metabolism, Mayo ClinicAbstract Despite indisputable benefits of different exercise modes, the molecular underpinnings of their divergent responses remain unclear. We investigate post-translational modifications in human skeletal muscle following 12 weeks of high-intensity aerobic interval or resistance exercise training. High-intensity aerobic training induces acetylproteome modifications including several mitochondrial proteins, indicating post-translational regulation of energetics machinery, whereas resistance exercise training regulates phosphoproteomic modifications of contractile/cytoskeletal machinery, consistent with greater strength. Furthermore, despite similar transcriptional responses to a single acute bout of aerobic and resistance exercise, more robust phosphoproteomic and metabolomic responses occur with acute aerobic exercise, including phosphorylation of structural/contractile and membrane transport machinery, and the nascent polypeptide-associated complex-α, a regulator of protein translation. Together, our findings provide new insight on the intricate phosphoproteomic and acetylproteomic modifications in muscle that potentially explain physiological responses to different modes of chronic and acute exercise. This study is registered with ClinicalTrials.gov, numbers NCT01477164 and NCT04158375.https://doi.org/10.1038/s41467-025-60049-0 |
| spellingShingle | Mark W. Pataky Carrie J. Heppelmann Kyle J. Sevits Aneesh K. Asokan Arathi Prabha Kumar Katherine A. Klaus Surendra Dasari Hawley E. Kunz Matthew D. Strub Matthew M. Robinson Joshua J. Coon Ian R. Lanza Christopher M. Adams K. Sreekumaran Nair Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle Nature Communications |
| title | Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle |
| title_full | Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle |
| title_fullStr | Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle |
| title_full_unstemmed | Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle |
| title_short | Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle |
| title_sort | aerobic and resistance exercise regulated phosphoproteome and acetylproteome modifications in human skeletal muscle |
| url | https://doi.org/10.1038/s41467-025-60049-0 |
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