Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi
A Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG1 and one IgG2a) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000450 |
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| author | T. L. Kipnis D. V. Tambourgi M. J. M. Alves W. Dias da Silva |
| author_facet | T. L. Kipnis D. V. Tambourgi M. J. M. Alves W. Dias da Silva |
| author_sort | T. L. Kipnis |
| collection | DOAJ |
| description | A Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG1 and one IgG2a) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies sensitize trypomastigotes and prepare them to activate the complement (C) system, as measured by consumption of total C, C4, B and C3, only the polyclonal antiserum or its IgG, IgM and Fabμ fragments were able to induce trypanosome lysis by the alternative C pathway. |
| format | Article |
| id | doaj-art-39cfc7f174444f4aa30a3fb66b20b4c8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-39cfc7f174444f4aa30a3fb66b20b4c82025-08-20T03:55:11ZengWileyMediators of Inflammation0962-93511466-18611992-01-011530931210.1155/S0962935192000450Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruziT. L. Kipnis0D. V. Tambourgi1M. J. M. Alves2W. Dias da Silva3Departamento de Imunologia, ICB, Universidade de São Paulo Av. Prof. Lineu Prestes 2415, São Paulo CEP 05508-900, BrazilDepartamento de Imunologia, ICB, Universidade de São Paulo Av. Prof. Lineu Prestes 2415, São Paulo CEP 05508-900, BrazilDepartamento de Bioquimica, IQ, Universidade de São Paulo Av. Prof. Lineu Prestes 748, São Paulo CEP 00508-970, BrazilLaboratório de Imunoquimica, Instituto Butantan Av. Vital Brazil 1500, São Paulo CEP 05504-900, BrazilA Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG1 and one IgG2a) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies sensitize trypomastigotes and prepare them to activate the complement (C) system, as measured by consumption of total C, C4, B and C3, only the polyclonal antiserum or its IgG, IgM and Fabμ fragments were able to induce trypanosome lysis by the alternative C pathway.http://dx.doi.org/10.1155/S0962935192000450 |
| spellingShingle | T. L. Kipnis D. V. Tambourgi M. J. M. Alves W. Dias da Silva Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi Mediators of Inflammation |
| title | Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi |
| title_full | Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi |
| title_fullStr | Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi |
| title_full_unstemmed | Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi |
| title_short | Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi |
| title_sort | comparison of the c mediating killing activity and c activating properties of mouse monoclonal and polyclonal antibodies against trypanosoma cruzi |
| url | http://dx.doi.org/10.1155/S0962935192000450 |
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