Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases

Aim: The high levels of anti-SSA/Ro and anti-SSB/La autoantibodies are closely associated with a group of diseases related to connective tissues, also known as connective tissue diseases (CTD). The current study attested to profile the multifactorial association between interleukin IL-6 and IL-10 in...

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Main Authors: Nor Effa S. Zulkafli, Anisah Abdul Zubir, Balqissiah Baharudin, Chuo Luan Tan, Nurul Aulia Zakaria, Ernest Mangantig
Format: Article
Language:English
Published: Open Exploration Publishing Inc. 2025-05-01
Series:Exploration of Immunology
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Online Access:https://www.explorationpub.com/uploads/Article/A1003197/1003197.pdf
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author Nor Effa S. Zulkafli
Anisah Abdul Zubir
Balqissiah Baharudin
Chuo Luan Tan
Nurul Aulia Zakaria
Ernest Mangantig
author_facet Nor Effa S. Zulkafli
Anisah Abdul Zubir
Balqissiah Baharudin
Chuo Luan Tan
Nurul Aulia Zakaria
Ernest Mangantig
author_sort Nor Effa S. Zulkafli
collection DOAJ
description Aim: The high levels of anti-SSA/Ro and anti-SSB/La autoantibodies are closely associated with a group of diseases related to connective tissues, also known as connective tissue diseases (CTD). The current study attested to profile the multifactorial association between interleukin IL-6 and IL-10 in sera from the study cohort to underline its putative prognostic and therapeutic characteristics for future application in CTD. Methods: The study cohort was recruited from government hospitals and screened for autoantibody using Enzyme Immunoassay (EIA) and Immunofluorescence Assay (IFA) while cytokine levels were measured using ELISA. Results: Our data showed the mean age of female patients is 38.1 years. Higher mean levels of both cytokines were observed in the first year of disease onset and menopause autoimmune-CTD patients. The mean levels of IL-6 and IL-10 were significantly higher in positive anti-Ro/La compared to the control group (p < 0.05). Also, the significant correlation of IL-6 and IL-10 in CTD patients as opposed to healthy control has underlined the putative role of these biologics. Conclusions: These data suggest the putative manipulation of IL-6 and IL-10 as prognostic and therapeutics molecules in managing CTD, as an alternative to steroid-based medications to control the disease manifestations.
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spelling doaj-art-39cc2dff6e184cd680acaf09da75d4ae2025-08-20T02:26:00ZengOpen Exploration Publishing Inc.Exploration of Immunology2768-66552025-05-015100319710.37349/ei.2025.1003197Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseasesNor Effa S. Zulkafli0https://orcid.org/0000-0003-4188-5506Anisah Abdul Zubir1Balqissiah Baharudin2Chuo Luan Tan3Nurul Aulia Zakaria4Ernest Mangantig5https://orcid.org/0000-0003-3355-3026Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang 13200, MalaysiaDepartment of Pathology, Hospital Tuanku Fauziah, Kangar 01000, Malaysia; Ministry of Health Malaysia, Federal Government Administrative Centre, Putrajaya 62590, MalaysiaDepartment of Pathology, Hospital Tuanku Fauziah, Kangar 01000, Malaysia; Ministry of Health Malaysia, Federal Government Administrative Centre, Putrajaya 62590, MalaysiaMinistry of Health Malaysia, Federal Government Administrative Centre, Putrajaya 62590, Malaysia; Department of Internal Medicine, Hospital Sultanah Bahiyah, Alor Setar 05460, MalaysiaMinistry of Health Malaysia, Federal Government Administrative Centre, Putrajaya 62590, Malaysia; Department of Internal Medicine, Hospital Sultanah Bahiyah, Alor Setar 05460, Malaysia; Department of Internal Medicine, Sultan Ahmad Shah Medical Centre, International Islamic University of Malaysia, Kuantan 2500, MalaysiaDepartment of Community Medicine, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang 13200, MalaysiaAim: The high levels of anti-SSA/Ro and anti-SSB/La autoantibodies are closely associated with a group of diseases related to connective tissues, also known as connective tissue diseases (CTD). The current study attested to profile the multifactorial association between interleukin IL-6 and IL-10 in sera from the study cohort to underline its putative prognostic and therapeutic characteristics for future application in CTD. Methods: The study cohort was recruited from government hospitals and screened for autoantibody using Enzyme Immunoassay (EIA) and Immunofluorescence Assay (IFA) while cytokine levels were measured using ELISA. Results: Our data showed the mean age of female patients is 38.1 years. Higher mean levels of both cytokines were observed in the first year of disease onset and menopause autoimmune-CTD patients. The mean levels of IL-6 and IL-10 were significantly higher in positive anti-Ro/La compared to the control group (p < 0.05). Also, the significant correlation of IL-6 and IL-10 in CTD patients as opposed to healthy control has underlined the putative role of these biologics. Conclusions: These data suggest the putative manipulation of IL-6 and IL-10 as prognostic and therapeutics molecules in managing CTD, as an alternative to steroid-based medications to control the disease manifestations.https://www.explorationpub.com/uploads/Article/A1003197/1003197.pdfil-6il-10anti-ro/anti-lainterleukinscytokineconnective tissue diseasesslerheumatoid arthritis
spellingShingle Nor Effa S. Zulkafli
Anisah Abdul Zubir
Balqissiah Baharudin
Chuo Luan Tan
Nurul Aulia Zakaria
Ernest Mangantig
Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases
Exploration of Immunology
il-6
il-10
anti-ro/anti-la
interleukins
cytokine
connective tissue diseases
sle
rheumatoid arthritis
title Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases
title_full Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases
title_fullStr Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases
title_full_unstemmed Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases
title_short Prognostic values of sera IL-6/IL-10 and high titration of anti-SSA/Ro and anti-SSB/La autoantibodies in female patients with connective tissue diseases
title_sort prognostic values of sera il 6 il 10 and high titration of anti ssa ro and anti ssb la autoantibodies in female patients with connective tissue diseases
topic il-6
il-10
anti-ro/anti-la
interleukins
cytokine
connective tissue diseases
sle
rheumatoid arthritis
url https://www.explorationpub.com/uploads/Article/A1003197/1003197.pdf
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