LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation
A rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitio...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8889375 |
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| author | Cao-Son Tran Quang-Dong Bui Ngoc-Tho Thi Nguyen Minh-Hanh Dao Thach-Tung Nguyen |
| author_facet | Cao-Son Tran Quang-Dong Bui Ngoc-Tho Thi Nguyen Minh-Hanh Dao Thach-Tung Nguyen |
| author_sort | Cao-Son Tran |
| collection | DOAJ |
| description | A rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitioning by a mixture of magnesium sulfate and sodium chloride. The supernatant was then directly injected into LC-MS/MS in a positive electrospray ionization mode and quantified using progesterone-d9 as the internal standard. The method linearity was in the range from 1 ng/mL (LOQ) to 200 ng/mL. Method recovery was from 86.0% to 103%, and repeatability was lower than 5.5%. The plasma sample was stable for 12 weeks stored at 18 ± 2°C. This method was applied to quantify progesterone in rabbit plasma in a pharmacokinetic study of two transdermal formulations: a reference drug and a eutectic-hydrogel system. The data indicate that the eutectic-hydrogel system’s bioavailability was 1.5 times better than that of the reference drug, and the transdermal system is a potential drug delivery system for progesterone. |
| format | Article |
| id | doaj-art-39c6fac2f65a4c2f8da59bd78e4d20f2 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-39c6fac2f65a4c2f8da59bd78e4d20f22025-08-20T02:24:08ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88893758889375LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal FormulationCao-Son Tran0Quang-Dong Bui1Ngoc-Tho Thi Nguyen2Minh-Hanh Dao3Thach-Tung Nguyen4Laboratory of Food Toxicology and Allergens Testing, National Institute for Food Control, Hanoi 10000, VietnamLaboratory of Food Toxicology and Allergens Testing, National Institute for Food Control, Hanoi 10000, VietnamDepartment of Pharmaceutics, Hanoi University of Pharmacy, Hanoi 10000, VietnamDepartment of Pharmaceutics, Hanoi University of Pharmacy, Hanoi 10000, VietnamDepartment of Pharmaceutics, Hanoi University of Pharmacy, Hanoi 10000, VietnamA rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma. The analyte was extracted from plasma by acetonitrile with phase partitioning by a mixture of magnesium sulfate and sodium chloride. The supernatant was then directly injected into LC-MS/MS in a positive electrospray ionization mode and quantified using progesterone-d9 as the internal standard. The method linearity was in the range from 1 ng/mL (LOQ) to 200 ng/mL. Method recovery was from 86.0% to 103%, and repeatability was lower than 5.5%. The plasma sample was stable for 12 weeks stored at 18 ± 2°C. This method was applied to quantify progesterone in rabbit plasma in a pharmacokinetic study of two transdermal formulations: a reference drug and a eutectic-hydrogel system. The data indicate that the eutectic-hydrogel system’s bioavailability was 1.5 times better than that of the reference drug, and the transdermal system is a potential drug delivery system for progesterone.http://dx.doi.org/10.1155/2020/8889375 |
| spellingShingle | Cao-Son Tran Quang-Dong Bui Ngoc-Tho Thi Nguyen Minh-Hanh Dao Thach-Tung Nguyen LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation Journal of Analytical Methods in Chemistry |
| title | LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
| title_full | LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
| title_fullStr | LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
| title_full_unstemmed | LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
| title_short | LC-MS/MS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation |
| title_sort | lc ms ms method for rapid quantification of progesterone in rabbit plasma and its application in a pharmacokinetic study of the transdermal formulation |
| url | http://dx.doi.org/10.1155/2020/8889375 |
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