Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface
Background: The gold standard treatment of periodontal diseases is scaling and root planing (SRP). Different adjunctive root conditioning agents such as hyaluronic acid (HA), ethylenediaminetetraacetic acid (EDTA), and chlorhexidine (CHX) have been used with SRP to improve the smear layer removal an...
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| Format: | Article |
| Language: | English |
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Springer
2021-09-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905219312568 |
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| author | Walaa Babgi Mashael Alhajaji Lujain Al-Mehmadi Rokaia Elbaqli Noha Khayat Salwa Aldahlawi Abdel-Rahman Youssef |
| author_facet | Walaa Babgi Mashael Alhajaji Lujain Al-Mehmadi Rokaia Elbaqli Noha Khayat Salwa Aldahlawi Abdel-Rahman Youssef |
| author_sort | Walaa Babgi |
| collection | DOAJ |
| description | Background: The gold standard treatment of periodontal diseases is scaling and root planing (SRP). Different adjunctive root conditioning agents such as hyaluronic acid (HA), ethylenediaminetetraacetic acid (EDTA), and chlorhexidine (CHX) have been used with SRP to improve the smear layer removal and the healing of periodontal tissues. The aim of this study was to compare the effect of manual scaling with or without HA, EDTA, or CHX root conditioning on the attachment and viability of human gingival fibroblasts (GF). Methods: Fifteen healthy single rooted teeth were co llected and divided randomly into a scaled (n = 12) and non-scaled control group (n = 3). The scaled roots were subdivided equally into four groups; the first group did not receive any chemical treatment, while the remaining groups were treated with the conditioning agents HA or 17% EDTA or 0.2% CHX gel. Gingival fibroblasts were seeded on the top of each root and incubated for 48 h to allow attachment to the roots. The viability of fibroblasts attached to the root surface was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. Results: The cell viability was the highest in the scaled only group (p = 0.0001) while the lowest was in the scaled with EDTA group (p > 0.05). The scaled group was the highest followed by the HA and CHX groups, while the EDTA group showed the lowest mean value. Conclusion: SRP remains the superior method for regaining cell attachment to the root surface, leading to better periodontal health, and adjunctive therapies did not enhance the GF attachment to the root surface beyond the effect of SRP. Further studies are needed to investigate the effect of root conditioning agents on periodontally diseased teeth in vitro and compare them in vivo. |
| format | Article |
| id | doaj-art-39c4402a888044d9a8d4e0620bd1aae6 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-39c4402a888044d9a8d4e0620bd1aae62025-08-20T02:13:48ZengSpringerSaudi Dental Journal1013-90522021-09-0133634234710.1016/j.sdentj.2020.03.009Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surfaceWalaa Babgi0Mashael Alhajaji1Lujain Al-Mehmadi2Rokaia Elbaqli3Noha Khayat4Salwa Aldahlawi5Abdel-Rahman Youssef6Dental Intern, Faculty of Dentistry, Umm Al-Qura University, Saudi ArabiaDental Intern, Faculty of Dentistry, Umm Al-Qura University, Saudi ArabiaDental Intern, Faculty of Dentistry, Umm Al-Qura University, Saudi ArabiaDental Intern, Faculty of Dentistry, Umm Al-Qura University, Saudi ArabiaDental Intern, Faculty of Dentistry, Umm Al-Qura University, Saudi ArabiaDepartment of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura University, Saudi ArabiaDepartment of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura University, Saudi Arabia; Department of Microbiology, Faculty of Medicine, Suez Canal University, Egypt; Corresponding author at: Faculty of Dentistry, Umm Al-Qura University, Al-Abdia Campus, Makkah, Saudi Arabia.Background: The gold standard treatment of periodontal diseases is scaling and root planing (SRP). Different adjunctive root conditioning agents such as hyaluronic acid (HA), ethylenediaminetetraacetic acid (EDTA), and chlorhexidine (CHX) have been used with SRP to improve the smear layer removal and the healing of periodontal tissues. The aim of this study was to compare the effect of manual scaling with or without HA, EDTA, or CHX root conditioning on the attachment and viability of human gingival fibroblasts (GF). Methods: Fifteen healthy single rooted teeth were co llected and divided randomly into a scaled (n = 12) and non-scaled control group (n = 3). The scaled roots were subdivided equally into four groups; the first group did not receive any chemical treatment, while the remaining groups were treated with the conditioning agents HA or 17% EDTA or 0.2% CHX gel. Gingival fibroblasts were seeded on the top of each root and incubated for 48 h to allow attachment to the roots. The viability of fibroblasts attached to the root surface was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. Results: The cell viability was the highest in the scaled only group (p = 0.0001) while the lowest was in the scaled with EDTA group (p > 0.05). The scaled group was the highest followed by the HA and CHX groups, while the EDTA group showed the lowest mean value. Conclusion: SRP remains the superior method for regaining cell attachment to the root surface, leading to better periodontal health, and adjunctive therapies did not enhance the GF attachment to the root surface beyond the effect of SRP. Further studies are needed to investigate the effect of root conditioning agents on periodontally diseased teeth in vitro and compare them in vivo.http://www.sciencedirect.com/science/article/pii/S1013905219312568Gingival fibroblastsHyaluronic AcidEDTAChlorhexidineCell viability |
| spellingShingle | Walaa Babgi Mashael Alhajaji Lujain Al-Mehmadi Rokaia Elbaqli Noha Khayat Salwa Aldahlawi Abdel-Rahman Youssef Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface Saudi Dental Journal Gingival fibroblasts Hyaluronic Acid EDTA Chlorhexidine Cell viability |
| title | Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface |
| title_full | Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface |
| title_fullStr | Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface |
| title_full_unstemmed | Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface |
| title_short | Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface |
| title_sort | effect of root conditioning agents hyaluronic acid edta and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface |
| topic | Gingival fibroblasts Hyaluronic Acid EDTA Chlorhexidine Cell viability |
| url | http://www.sciencedirect.com/science/article/pii/S1013905219312568 |
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