Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy
Breast cancer (BC) has a prevalence rate of 21.8% among Saudi women and ranks as the third leading cause of death in Western nations. Nanotechnology offers innovative methods for targeted BC therapy, and this study explores the use of single-walled carbon nanotubes (SWCNTs) for delivering the senna...
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-12-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-0994 |
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| author | Behairy Sabreen Mohammed Al-Maaqar Saleh Mohammed Al-Shaeri Majed Ahmed |
| author_facet | Behairy Sabreen Mohammed Al-Maaqar Saleh Mohammed Al-Shaeri Majed Ahmed |
| author_sort | Behairy Sabreen Mohammed |
| collection | DOAJ |
| description | Breast cancer (BC) has a prevalence rate of 21.8% among Saudi women and ranks as the third leading cause of death in Western nations. Nanotechnology offers innovative methods for targeted BC therapy, and this study explores the use of single-walled carbon nanotubes (SWCNTs) for delivering the senna leaf extract. The study evaluated the effects of increasing dosages of senna leaf extract conjugated to SWCNTs on MCF-7 cells. Cell viability was assessed using the MTT assay, while Giemsa staining revealed morphological changes. Additionally, the comet assay and agarose gel electrophoresis were employed to evaluate the pro-apoptotic potential. The potential of mitochondrial membrane and the production of reactive oxygen species (ROS) were investigated using the JC-1 dye. The results indicated that treated cells exhibited apoptotic characteristics, including elevated ROS levels and decreased mitochondrial membrane potential. In summary, the application of nanotechnology to deliver the senna leaf extract shows promise as a herbal treatment for BC, suggesting a potential breakthrough in combating this widespread and deadly disease. |
| format | Article |
| id | doaj-art-39c0f237aec7417a93bf156970431e60 |
| institution | Kabale University |
| issn | 2391-5412 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj-art-39c0f237aec7417a93bf156970431e602025-01-07T07:55:32ZengDe GruyterOpen Life Sciences2391-54122024-12-01191e2023014687610.1515/biol-2022-0994Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategyBehairy Sabreen Mohammed0Al-Maaqar Saleh Mohammed1Al-Shaeri Majed Ahmed2Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaBreast cancer (BC) has a prevalence rate of 21.8% among Saudi women and ranks as the third leading cause of death in Western nations. Nanotechnology offers innovative methods for targeted BC therapy, and this study explores the use of single-walled carbon nanotubes (SWCNTs) for delivering the senna leaf extract. The study evaluated the effects of increasing dosages of senna leaf extract conjugated to SWCNTs on MCF-7 cells. Cell viability was assessed using the MTT assay, while Giemsa staining revealed morphological changes. Additionally, the comet assay and agarose gel electrophoresis were employed to evaluate the pro-apoptotic potential. The potential of mitochondrial membrane and the production of reactive oxygen species (ROS) were investigated using the JC-1 dye. The results indicated that treated cells exhibited apoptotic characteristics, including elevated ROS levels and decreased mitochondrial membrane potential. In summary, the application of nanotechnology to deliver the senna leaf extract shows promise as a herbal treatment for BC, suggesting a potential breakthrough in combating this widespread and deadly disease.https://doi.org/10.1515/biol-2022-0994breast cancerdrug deliverysenna leaf extractscarbon nanotubesdna damagecell viability |
| spellingShingle | Behairy Sabreen Mohammed Al-Maaqar Saleh Mohammed Al-Shaeri Majed Ahmed Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy Open Life Sciences breast cancer drug delivery senna leaf extracts carbon nanotubes dna damage cell viability |
| title | Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy |
| title_full | Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy |
| title_fullStr | Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy |
| title_full_unstemmed | Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy |
| title_short | Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy |
| title_sort | impact of swcnt conjugated senna leaf extract on breast cancer cells a potential apoptotic therapeutic strategy |
| topic | breast cancer drug delivery senna leaf extracts carbon nanotubes dna damage cell viability |
| url | https://doi.org/10.1515/biol-2022-0994 |
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