Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes
Abstract Nanoelectrode arrays (NEAs) are emerging as promising technologies for minimally‐invasive, parallel intracellular recording. These vertical electrodes, typically hundreds of nanometers in diameter and micrometers in height, provide a means for gentle electroporation and reversible membrane...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-07-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202500187 |
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| author | Ching‐Ting Tsai Hongyan Gao Csaba Forro Yang Yang Viktoriya Shautsova Xingyuan Zhang Zeinab Jahed Bianxiao Cui |
| author_facet | Ching‐Ting Tsai Hongyan Gao Csaba Forro Yang Yang Viktoriya Shautsova Xingyuan Zhang Zeinab Jahed Bianxiao Cui |
| author_sort | Ching‐Ting Tsai |
| collection | DOAJ |
| description | Abstract Nanoelectrode arrays (NEAs) are emerging as promising technologies for minimally‐invasive, parallel intracellular recording. These vertical electrodes, typically hundreds of nanometers in diameter and micrometers in height, provide a means for gentle electroporation and reversible membrane permeabilization to achieve intracellular recording. Prior studies have used 5–9 vertical nanostructures per recording channel to enhance device robustness and signal strength. However, this approach complicates the establishment of a one‐to‐one correspondence between cells and electrodes. In this study, devices with recording channels featuring 1‐, 3‐, 5‐, or 9‐ vertical nanocrowns electrode arrays (NcEAs) are developed in the same device. Channels with vertical nanoelectrodes of different geometries, as well as non‐vertical electrodes, such as shallow hole electrodes and large flat electrodes, are also incorporated. These measurements demonstrate that a single NcEA not only provides high‐quality iAP recordings but also excels at preserving the intracellular waveform. In contrast, non‐vertical electrodes detect intracellular‐like signals with distorted waveforms and are not suitable for cardiac intracellular recordings. These findings highlight the critical role of electrode geometry in improving the precision and reliability of intracellular recording technologies. |
| format | Article |
| id | doaj-art-39bdfeae9f6b4d60a6a2e1194e151621 |
| institution | Kabale University |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-39bdfeae9f6b4d60a6a2e1194e1516212025-08-20T03:58:36ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-07-011214n/an/a10.1002/admi.202500187Single Nanocrown Electrodes for High‐Quality Intracellular Recording of CardiomyocytesChing‐Ting Tsai0Hongyan Gao1Csaba Forro2Yang Yang3Viktoriya Shautsova4Xingyuan Zhang5Zeinab Jahed6Bianxiao Cui7Department of Chemistry Stanford University Stanford CA 94305 USADepartment of Chemistry Stanford University Stanford CA 94305 USADepartment of Chemistry Stanford University Stanford CA 94305 USADepartment of Chemistry Stanford University Stanford CA 94305 USADepartment of Chemistry Stanford University Stanford CA 94305 USADepartment of Chemistry Stanford University Stanford CA 94305 USAAiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California San Diego La Jolla CA 92093 USADepartment of Chemistry Stanford University Stanford CA 94305 USAAbstract Nanoelectrode arrays (NEAs) are emerging as promising technologies for minimally‐invasive, parallel intracellular recording. These vertical electrodes, typically hundreds of nanometers in diameter and micrometers in height, provide a means for gentle electroporation and reversible membrane permeabilization to achieve intracellular recording. Prior studies have used 5–9 vertical nanostructures per recording channel to enhance device robustness and signal strength. However, this approach complicates the establishment of a one‐to‐one correspondence between cells and electrodes. In this study, devices with recording channels featuring 1‐, 3‐, 5‐, or 9‐ vertical nanocrowns electrode arrays (NcEAs) are developed in the same device. Channels with vertical nanoelectrodes of different geometries, as well as non‐vertical electrodes, such as shallow hole electrodes and large flat electrodes, are also incorporated. These measurements demonstrate that a single NcEA not only provides high‐quality iAP recordings but also excels at preserving the intracellular waveform. In contrast, non‐vertical electrodes detect intracellular‐like signals with distorted waveforms and are not suitable for cardiac intracellular recordings. These findings highlight the critical role of electrode geometry in improving the precision and reliability of intracellular recording technologies.https://doi.org/10.1002/admi.202500187cardiomyocyteselectroporationintracellular action potentialnanocrown electrodenanofabrication |
| spellingShingle | Ching‐Ting Tsai Hongyan Gao Csaba Forro Yang Yang Viktoriya Shautsova Xingyuan Zhang Zeinab Jahed Bianxiao Cui Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes Advanced Materials Interfaces cardiomyocytes electroporation intracellular action potential nanocrown electrode nanofabrication |
| title | Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes |
| title_full | Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes |
| title_fullStr | Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes |
| title_full_unstemmed | Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes |
| title_short | Single Nanocrown Electrodes for High‐Quality Intracellular Recording of Cardiomyocytes |
| title_sort | single nanocrown electrodes for high quality intracellular recording of cardiomyocytes |
| topic | cardiomyocytes electroporation intracellular action potential nanocrown electrode nanofabrication |
| url | https://doi.org/10.1002/admi.202500187 |
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