In situ optical mapping of voltage and calcium in the heart.
Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological funct...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042562&type=printable |
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| author | Peter Lee Fouad Taghavi Ping Yan Paul Ewart Euan A Ashley Leslie M Loew Peter Kohl Christian Bollensdorff Christopher E Woods |
| author_facet | Peter Lee Fouad Taghavi Ping Yan Paul Ewart Euan A Ashley Leslie M Loew Peter Kohl Christian Bollensdorff Christopher E Woods |
| author_sort | Peter Lee |
| collection | DOAJ |
| description | Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches. |
| format | Article |
| id | doaj-art-39bac620917a41cea991c05e1b157bb9 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-39bac620917a41cea991c05e1b157bb92025-08-20T02:05:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4256210.1371/journal.pone.0042562In situ optical mapping of voltage and calcium in the heart.Peter LeeFouad TaghaviPing YanPaul EwartEuan A AshleyLeslie M LoewPeter KohlChristian BollensdorffChristopher E WoodsElectroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042562&type=printable |
| spellingShingle | Peter Lee Fouad Taghavi Ping Yan Paul Ewart Euan A Ashley Leslie M Loew Peter Kohl Christian Bollensdorff Christopher E Woods In situ optical mapping of voltage and calcium in the heart. PLoS ONE |
| title | In situ optical mapping of voltage and calcium in the heart. |
| title_full | In situ optical mapping of voltage and calcium in the heart. |
| title_fullStr | In situ optical mapping of voltage and calcium in the heart. |
| title_full_unstemmed | In situ optical mapping of voltage and calcium in the heart. |
| title_short | In situ optical mapping of voltage and calcium in the heart. |
| title_sort | in situ optical mapping of voltage and calcium in the heart |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042562&type=printable |
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