Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies
Induced pluripotent stem cells (iPSCs) are generated through a gradual process in which somatic cells undergo a number of stochastic events. In this study, we examined whether two different doxycycline-inducible iPSCs, slow-forming 4F2A-iPSCs and fast-forming NGFP-iPSCs, have equivalent levels of pl...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/195928 |
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| author | Sujin Kwon Jung Sun Park Byungkuk Min Yong-Kook Kang |
| author_facet | Sujin Kwon Jung Sun Park Byungkuk Min Yong-Kook Kang |
| author_sort | Sujin Kwon |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) are generated through a gradual process in which somatic cells undergo a number of stochastic events. In this study, we examined whether two different doxycycline-inducible iPSCs, slow-forming 4F2A-iPSCs and fast-forming NGFP-iPSCs, have equivalent levels of pluripotency. Multiplex reverse-transcriptase PCR generated gene expression profiles (GEPs) of 13 pluripotency genes in single initially formed-iPSC (if-iPSC) colonies of NGFP and 4F2A group. Assessment of GEP difference using a weighted root mean square deviation (wRMSD) indicates that 4F2A if-iPSCs are more closely related to mESCs than NGFP if-iPSCs. Consistently, Nanog and Sox2 genes were more frequently derepressed in 4F2A if-iPSC group. We further examined 20 genes that are implicated in reprogramming. They were, overall, more highly expressed in NGFP if-iPSCs, differing from the pluripotency genes being more expressed in 4F2A if-iPSCs. wRMSD analysis for these reprogramming-related genes confirmed that the 4F2A if-iPSC colonies were less deviated from mESCs than the NGFP if-iPSC colonies. Our findings suggest that more important in attaining a better reprogramming is the mode of action by the given reprogramming factors, rather than the total activity of them exerting to the cells, as the thin-but-long-lasting mode of action in 4F2A if-iPSCs is shown to be more effective than its full-but-short-lasting mode in NGFP if-iPSCs. |
| format | Article |
| id | doaj-art-39b0659607aa4b31bb625b0cbc86b9de |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-39b0659607aa4b31bb625b0cbc86b9de2025-02-03T05:53:00ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/195928195928Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell ColoniesSujin Kwon0Jung Sun Park1Byungkuk Min2Yong-Kook Kang3Development and Differentiation Research Center, KRIBB, 111 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of KoreaDevelopment and Differentiation Research Center, KRIBB, 111 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of KoreaDevelopment and Differentiation Research Center, KRIBB, 111 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of KoreaDevelopment and Differentiation Research Center, KRIBB, 111 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of KoreaInduced pluripotent stem cells (iPSCs) are generated through a gradual process in which somatic cells undergo a number of stochastic events. In this study, we examined whether two different doxycycline-inducible iPSCs, slow-forming 4F2A-iPSCs and fast-forming NGFP-iPSCs, have equivalent levels of pluripotency. Multiplex reverse-transcriptase PCR generated gene expression profiles (GEPs) of 13 pluripotency genes in single initially formed-iPSC (if-iPSC) colonies of NGFP and 4F2A group. Assessment of GEP difference using a weighted root mean square deviation (wRMSD) indicates that 4F2A if-iPSCs are more closely related to mESCs than NGFP if-iPSCs. Consistently, Nanog and Sox2 genes were more frequently derepressed in 4F2A if-iPSC group. We further examined 20 genes that are implicated in reprogramming. They were, overall, more highly expressed in NGFP if-iPSCs, differing from the pluripotency genes being more expressed in 4F2A if-iPSCs. wRMSD analysis for these reprogramming-related genes confirmed that the 4F2A if-iPSC colonies were less deviated from mESCs than the NGFP if-iPSC colonies. Our findings suggest that more important in attaining a better reprogramming is the mode of action by the given reprogramming factors, rather than the total activity of them exerting to the cells, as the thin-but-long-lasting mode of action in 4F2A if-iPSCs is shown to be more effective than its full-but-short-lasting mode in NGFP if-iPSCs.http://dx.doi.org/10.1155/2015/195928 |
| spellingShingle | Sujin Kwon Jung Sun Park Byungkuk Min Yong-Kook Kang Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies Stem Cells International |
| title | Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies |
| title_full | Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies |
| title_fullStr | Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies |
| title_full_unstemmed | Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies |
| title_short | Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies |
| title_sort | differences in the gene expression profiles of slow and fast forming preinduced pluripotent stem cell colonies |
| url | http://dx.doi.org/10.1155/2015/195928 |
| work_keys_str_mv | AT sujinkwon differencesinthegeneexpressionprofilesofslowandfastformingpreinducedpluripotentstemcellcolonies AT jungsunpark differencesinthegeneexpressionprofilesofslowandfastformingpreinducedpluripotentstemcellcolonies AT byungkukmin differencesinthegeneexpressionprofilesofslowandfastformingpreinducedpluripotentstemcellcolonies AT yongkookkang differencesinthegeneexpressionprofilesofslowandfastformingpreinducedpluripotentstemcellcolonies |