Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy
Abstract Many plant lncRNAs regulate gene expression by binding to chromatin, but how they are retained at the target loci is unclear. We identify a chromatin-localized lncRNA - MUSHER, which activates two parallel regulatory pathways to increase Arabidopsis seed dormancy. MUSHER is upregulated in r...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-62991-5 |
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| author | Sebastian Przemyslaw Sacharowski Michal Krzyszton Lien Brzezniak Karol Jerzy Rogowski Miguel Montez Katarzyna Rosol Magdalena Wrona Ruslan Yatusevich Veena Halale Manjunath Lukasz Szewc Artur Jarmolowski Szymon Swiezewski |
| author_facet | Sebastian Przemyslaw Sacharowski Michal Krzyszton Lien Brzezniak Karol Jerzy Rogowski Miguel Montez Katarzyna Rosol Magdalena Wrona Ruslan Yatusevich Veena Halale Manjunath Lukasz Szewc Artur Jarmolowski Szymon Swiezewski |
| author_sort | Sebastian Przemyslaw Sacharowski |
| collection | DOAJ |
| description | Abstract Many plant lncRNAs regulate gene expression by binding to chromatin, but how they are retained at the target loci is unclear. We identify a chromatin-localized lncRNA - MUSHER, which activates two parallel regulatory pathways to increase Arabidopsis seed dormancy. MUSHER is upregulated in response to high temperatures, contributing to the induction of secondary dormancy. It promotes DOG1 expression by recruitment of the CPSF complex to enhance the proximal cleavage and polyadenylation at the DOG1 transcript. It also increases ABA sensitivity in seeds by activating PIR1 gene transcription. These genes, located on different chromosomes, are both bound by MUSHER, despite lacking sequence homology. The chromatin association of MUSHER enables the integration of the DOG1- and ABA pathways to adjust seed germination timing. Additionally, MUSHER and other lncRNAs interact with U1 snRNP, which is required for their chromatin localisation, revealing a role for U1 snRNP in plants. |
| format | Article |
| id | doaj-art-39af623d5aa144a390ff6399573a3fbc |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-39af623d5aa144a390ff6399573a3fbc2025-08-20T04:02:54ZengNature PortfolioNature Communications2041-17232025-08-0116111810.1038/s41467-025-62991-5Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancySebastian Przemyslaw Sacharowski0Michal Krzyszton1Lien Brzezniak2Karol Jerzy Rogowski3Miguel Montez4Katarzyna Rosol5Magdalena Wrona6Ruslan Yatusevich7Veena Halale Manjunath8Lukasz Szewc9Artur Jarmolowski10Szymon Swiezewski11Laboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASElementZero BiolabsDepartment of Cell and Developmental Biology, John Innes Centre, Norwich Research ParkLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz UniversityDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz UniversityLaboratory of Seed Molecular Biology, Institute of Biochemistry and Biophysics PASAbstract Many plant lncRNAs regulate gene expression by binding to chromatin, but how they are retained at the target loci is unclear. We identify a chromatin-localized lncRNA - MUSHER, which activates two parallel regulatory pathways to increase Arabidopsis seed dormancy. MUSHER is upregulated in response to high temperatures, contributing to the induction of secondary dormancy. It promotes DOG1 expression by recruitment of the CPSF complex to enhance the proximal cleavage and polyadenylation at the DOG1 transcript. It also increases ABA sensitivity in seeds by activating PIR1 gene transcription. These genes, located on different chromosomes, are both bound by MUSHER, despite lacking sequence homology. The chromatin association of MUSHER enables the integration of the DOG1- and ABA pathways to adjust seed germination timing. Additionally, MUSHER and other lncRNAs interact with U1 snRNP, which is required for their chromatin localisation, revealing a role for U1 snRNP in plants.https://doi.org/10.1038/s41467-025-62991-5 |
| spellingShingle | Sebastian Przemyslaw Sacharowski Michal Krzyszton Lien Brzezniak Karol Jerzy Rogowski Miguel Montez Katarzyna Rosol Magdalena Wrona Ruslan Yatusevich Veena Halale Manjunath Lukasz Szewc Artur Jarmolowski Szymon Swiezewski Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy Nature Communications |
| title | Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy |
| title_full | Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy |
| title_fullStr | Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy |
| title_full_unstemmed | Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy |
| title_short | Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy |
| title_sort | chromatin retained musher lncrna integrates aba and dog1 signalling pathways to enhance arabidopsis seeds dormancy |
| url | https://doi.org/10.1038/s41467-025-62991-5 |
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