Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish

Primary liver cancer (PLC) is the sixth most common tumour disease and one of the leading causes of cancer-related death worldwide. The two most common types of PLC are hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). Diverse subgroups are described and a manifold number of...

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Main Authors: Umesh Tharehalli, Michael Svinarenko, André Lechel
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2019/3831213
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author Umesh Tharehalli
Michael Svinarenko
André Lechel
author_facet Umesh Tharehalli
Michael Svinarenko
André Lechel
author_sort Umesh Tharehalli
collection DOAJ
description Primary liver cancer (PLC) is the sixth most common tumour disease and one of the leading causes of cancer-related death worldwide. The two most common types of PLC are hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). Diverse subgroups are described and a manifold number of gene mutations are known. Asymptomatic disease progression and limited therapeutic options are the reasons for the high mortality rate in PLC. Up to date, the multikinase inhibitors sorafenib and lenvatinib are the only FDA-approved first-line treatments for advanced HCC. One of the major drawbacks in the preclinical drug development is the lack of suitable model systems. In recent years, 3D organoid cultures were established from several organs and tumour subtypes, thereby opening new avenues in tumour research. 3D organoid cultures are used to describe the tumour diversity, for cancer modelling in a dish and for therapy responsiveness. The establishment of living biobanks and the development of next-generation matrices are promising approaches to overcome drug resistance and to improve the quality of personalised anticancer strategies for patients with PLC. In this review, we summarise the current knowledge of 3D cultures generated from healthy liver and primary liver cancer.
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spelling doaj-art-39a6c52881384f2f9deae0c3b2d855722025-08-20T03:55:12ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/38312133831213Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a DishUmesh Tharehalli0Michael Svinarenko1André Lechel2Department of Internal Medicine I, Ulm University, Ulm, GermanyDepartment of Internal Medicine I, Ulm University, Ulm, GermanyDepartment of Internal Medicine I, Ulm University, Ulm, GermanyPrimary liver cancer (PLC) is the sixth most common tumour disease and one of the leading causes of cancer-related death worldwide. The two most common types of PLC are hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). Diverse subgroups are described and a manifold number of gene mutations are known. Asymptomatic disease progression and limited therapeutic options are the reasons for the high mortality rate in PLC. Up to date, the multikinase inhibitors sorafenib and lenvatinib are the only FDA-approved first-line treatments for advanced HCC. One of the major drawbacks in the preclinical drug development is the lack of suitable model systems. In recent years, 3D organoid cultures were established from several organs and tumour subtypes, thereby opening new avenues in tumour research. 3D organoid cultures are used to describe the tumour diversity, for cancer modelling in a dish and for therapy responsiveness. The establishment of living biobanks and the development of next-generation matrices are promising approaches to overcome drug resistance and to improve the quality of personalised anticancer strategies for patients with PLC. In this review, we summarise the current knowledge of 3D cultures generated from healthy liver and primary liver cancer.http://dx.doi.org/10.1155/2019/3831213
spellingShingle Umesh Tharehalli
Michael Svinarenko
André Lechel
Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish
Stem Cells International
title Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish
title_full Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish
title_fullStr Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish
title_full_unstemmed Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish
title_short Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish
title_sort remodelling and improvements in organoid technology to study liver carcinogenesis in a dish
url http://dx.doi.org/10.1155/2019/3831213
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AT michaelsvinarenko remodellingandimprovementsinorganoidtechnologytostudylivercarcinogenesisinadish
AT andrelechel remodellingandimprovementsinorganoidtechnologytostudylivercarcinogenesisinadish