Clinical possibilities of flow cytometry in hodgkin’s lymphoma
Hodgkin’s lymphoma (HL) is a unique lymphoma of b-cell origin, the tumor cells of which have lost the expression of main b-cell antigens. The standard immunophenotype of the tumor substrate, according to immunohistochemical studies, is characterized by the expression of such markers as CD15, CD30 an...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
ABV-press
2023-09-01
|
| Series: | Онкогематология |
| Subjects: | |
| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/838 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850024765529522176 |
|---|---|
| author | F. M. Abbasbeyli A. A. Fedenko P. A. Zeynalova R. F. Zibirov V. N. Grinevich T. Yu. Mushkarina A. A. Melnikova M. A. Vernyuk L. Yu. Grivtsova |
| author_facet | F. M. Abbasbeyli A. A. Fedenko P. A. Zeynalova R. F. Zibirov V. N. Grinevich T. Yu. Mushkarina A. A. Melnikova M. A. Vernyuk L. Yu. Grivtsova |
| author_sort | F. M. Abbasbeyli |
| collection | DOAJ |
| description | Hodgkin’s lymphoma (HL) is a unique lymphoma of b-cell origin, the tumor cells of which have lost the expression of main b-cell antigens. The standard immunophenotype of the tumor substrate, according to immunohistochemical studies, is characterized by the expression of such markers as CD15, CD30 and PAX-5, while tumor cells have a negative expression of CD3, CD19 and in most cases also CD20 and CD45 (or express this antigen rather weakly). The knowledge gained in recent years has increased the effectiveness of diagnosis, prognosis and treatment of LH. Flow cytometry, as a method of immunophenotyping in classical LH, was practically not used due to the difficulty of distinguishing single reed–sternberg–berezovsky tumor cells (RSB) and the tumor microenvironment (reactive background), which is very rich in cellular elements and is represented by T cells, B cells, eosinophils, histiocytes and plasma cells. However, in the recent past, several studies have successfully attempted to identify rsb cells using multiparameter flow cytometry during aspiration with a thin needle or biopsy of lymph node tissue to confirm or supplement immunohistochemical staining during primary diagnosis. Taking into account the very characteristic immunophenotype of RSB cells, the flow cytometry may become an additional diagnostic method of classical LH in the future. In this review, we summarize the data on the possibility of using the flow cytometry as an additional clinical diagnostic option in the primary diagnosis of classical LH. |
| format | Article |
| id | doaj-art-39a21135b8514f22b01aa5f24ea5fe50 |
| institution | DOAJ |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2023-09-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-39a21135b8514f22b01aa5f24ea5fe502025-08-20T03:01:02ZrusABV-pressОнкогематология1818-83462413-40232023-09-01183707710.17650/1818-8346-2023-18-3-70-77699Clinical possibilities of flow cytometry in hodgkin’s lymphomaF. M. Abbasbeyli0A. A. Fedenko1P. A. Zeynalova2R. F. Zibirov3V. N. Grinevich4T. Yu. Mushkarina5A. A. Melnikova6M. A. Vernyuk7L. Yu. Grivtsova8Clinical Hospital “Lapino” of the “Mother and Child” Group of companiesClinical Hospital “Lapino” of the “Mother and Child” Group of companies; P. A. Hertzen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaClinical Hospital “Lapino” of the “Mother and Child” Group of companies; I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)A. F. Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaP. A. Hertzen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaA. F. Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaA. F. Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaP. A. Hertzen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaA. F. Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaHodgkin’s lymphoma (HL) is a unique lymphoma of b-cell origin, the tumor cells of which have lost the expression of main b-cell antigens. The standard immunophenotype of the tumor substrate, according to immunohistochemical studies, is characterized by the expression of such markers as CD15, CD30 and PAX-5, while tumor cells have a negative expression of CD3, CD19 and in most cases also CD20 and CD45 (or express this antigen rather weakly). The knowledge gained in recent years has increased the effectiveness of diagnosis, prognosis and treatment of LH. Flow cytometry, as a method of immunophenotyping in classical LH, was practically not used due to the difficulty of distinguishing single reed–sternberg–berezovsky tumor cells (RSB) and the tumor microenvironment (reactive background), which is very rich in cellular elements and is represented by T cells, B cells, eosinophils, histiocytes and plasma cells. However, in the recent past, several studies have successfully attempted to identify rsb cells using multiparameter flow cytometry during aspiration with a thin needle or biopsy of lymph node tissue to confirm or supplement immunohistochemical staining during primary diagnosis. Taking into account the very characteristic immunophenotype of RSB cells, the flow cytometry may become an additional diagnostic method of classical LH in the future. In this review, we summarize the data on the possibility of using the flow cytometry as an additional clinical diagnostic option in the primary diagnosis of classical LH.https://oncohematology.abvpress.ru/ongm/article/view/838hodgkin’s lymphomareed–sternberg–berezovsky tumor cellflow cytometry |
| spellingShingle | F. M. Abbasbeyli A. A. Fedenko P. A. Zeynalova R. F. Zibirov V. N. Grinevich T. Yu. Mushkarina A. A. Melnikova M. A. Vernyuk L. Yu. Grivtsova Clinical possibilities of flow cytometry in hodgkin’s lymphoma Онкогематология hodgkin’s lymphoma reed–sternberg–berezovsky tumor cell flow cytometry |
| title | Clinical possibilities of flow cytometry in hodgkin’s lymphoma |
| title_full | Clinical possibilities of flow cytometry in hodgkin’s lymphoma |
| title_fullStr | Clinical possibilities of flow cytometry in hodgkin’s lymphoma |
| title_full_unstemmed | Clinical possibilities of flow cytometry in hodgkin’s lymphoma |
| title_short | Clinical possibilities of flow cytometry in hodgkin’s lymphoma |
| title_sort | clinical possibilities of flow cytometry in hodgkin s lymphoma |
| topic | hodgkin’s lymphoma reed–sternberg–berezovsky tumor cell flow cytometry |
| url | https://oncohematology.abvpress.ru/ongm/article/view/838 |
| work_keys_str_mv | AT fmabbasbeyli clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT aafedenko clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT pazeynalova clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT rfzibirov clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT vngrinevich clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT tyumushkarina clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT aamelnikova clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT mavernyuk clinicalpossibilitiesofflowcytometryinhodgkinslymphoma AT lyugrivtsova clinicalpossibilitiesofflowcytometryinhodgkinslymphoma |