Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain
A bacterial strain (No. 20230510) was isolated from the kidneys of diseased Siniperca chuatsi in Guangxi, China, since 2023. Artificial infection experiments demonstrated that this strain caused the observed disease in S. chuatsi. The isolate underwent morphological, pathological, genomic (whole-gen...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1623741/full |
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| author | Liting Chen Liting Chen Xin Yan Xin Yan Yongju Luo Yongju Luo Zhuanling Lu Zhuanling Lu Xinxian Wei Xinxian Wei Zhanyang Tang Zhanyang Tang Liqun Xia Fuyan Chen Fuyan Chen Ming Li Ming Li Zhongbao Guo Zhongbao Guo Zhichang He Zhichang He Ting Huang Ting Huang |
| author_facet | Liting Chen Liting Chen Xin Yan Xin Yan Yongju Luo Yongju Luo Zhuanling Lu Zhuanling Lu Xinxian Wei Xinxian Wei Zhanyang Tang Zhanyang Tang Liqun Xia Fuyan Chen Fuyan Chen Ming Li Ming Li Zhongbao Guo Zhongbao Guo Zhichang He Zhichang He Ting Huang Ting Huang |
| author_sort | Liting Chen |
| collection | DOAJ |
| description | A bacterial strain (No. 20230510) was isolated from the kidneys of diseased Siniperca chuatsi in Guangxi, China, since 2023. Artificial infection experiments demonstrated that this strain caused the observed disease in S. chuatsi. The isolate underwent morphological, pathological, genomic (whole-genome sequencing, WGS), and antibiotic susceptibility analyses. Infection trials revealed 100% mortality in high-concentration groups, with an LD50 of 3.89 × 104 CFU/mL, indicating high virulence. WGS results showed a circular chromosome of 8,123,106 bp (GC content: 68.14%), containing 7,638 CDSs, 72 tRNAs, and 12 rRNAs. Phylogenomic analysis revealed that strain 20230510 (CP130742) clusters with three N. seriolae strains with 98% bootstrap supporting, confirming its identification as N. seriolae. Further analysis identified 403 potential virulence genes linked to nutrient metabolism, regulatory factors, immune modulation, effector delivery systems, and exotoxins. Chromosomal comparisons also detected multiple antibiotic resistance genes. Susceptibility testing confirmed sensitivity to nine antibiotics, including enrofloxacin, doxycycline, florfenicol, and sulfamethoxazole. Histopathology revealed chronic granulomatous lesions, most severe in the kidneys, with similar but milder damage in the liver, spleen, gills, and intestines. These results confirm N. seriolae strain 20230510 as the pathogenic agent behind S. chuatsi mortality, offering key insights for developing control strategies. |
| format | Article |
| id | doaj-art-3993111202e4493c931ad62383ff767c |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-3993111202e4493c931ad62383ff767c2025-08-25T05:25:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.16237411623741Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strainLiting Chen0Liting Chen1Xin Yan2Xin Yan3Yongju Luo4Yongju Luo5Zhuanling Lu6Zhuanling Lu7Xinxian Wei8Xinxian Wei9Zhanyang Tang10Zhanyang Tang11Liqun Xia12Fuyan Chen13Fuyan Chen14Ming Li15Ming Li16Zhongbao Guo17Zhongbao Guo18Zhichang He19Zhichang He20Ting Huang21Ting Huang22Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Shenzhen Institute of Guangdong Ocean University, Guangdong, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Science, Nanning, Guangxi, ChinaKey Laboratory of Comprehensive Development and Utilization of Aquatic Germplasm Resources of China (Guangxi) and ASEAN (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi, ChinaA bacterial strain (No. 20230510) was isolated from the kidneys of diseased Siniperca chuatsi in Guangxi, China, since 2023. Artificial infection experiments demonstrated that this strain caused the observed disease in S. chuatsi. The isolate underwent morphological, pathological, genomic (whole-genome sequencing, WGS), and antibiotic susceptibility analyses. Infection trials revealed 100% mortality in high-concentration groups, with an LD50 of 3.89 × 104 CFU/mL, indicating high virulence. WGS results showed a circular chromosome of 8,123,106 bp (GC content: 68.14%), containing 7,638 CDSs, 72 tRNAs, and 12 rRNAs. Phylogenomic analysis revealed that strain 20230510 (CP130742) clusters with three N. seriolae strains with 98% bootstrap supporting, confirming its identification as N. seriolae. Further analysis identified 403 potential virulence genes linked to nutrient metabolism, regulatory factors, immune modulation, effector delivery systems, and exotoxins. Chromosomal comparisons also detected multiple antibiotic resistance genes. Susceptibility testing confirmed sensitivity to nine antibiotics, including enrofloxacin, doxycycline, florfenicol, and sulfamethoxazole. Histopathology revealed chronic granulomatous lesions, most severe in the kidneys, with similar but milder damage in the liver, spleen, gills, and intestines. These results confirm N. seriolae strain 20230510 as the pathogenic agent behind S. chuatsi mortality, offering key insights for developing control strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1623741/fullSiniperca chuatsiNocardia seriolaepathogenicitywhole-genome analysisantibiotic susceptibility testing |
| spellingShingle | Liting Chen Liting Chen Xin Yan Xin Yan Yongju Luo Yongju Luo Zhuanling Lu Zhuanling Lu Xinxian Wei Xinxian Wei Zhanyang Tang Zhanyang Tang Liqun Xia Fuyan Chen Fuyan Chen Ming Li Ming Li Zhongbao Guo Zhongbao Guo Zhichang He Zhichang He Ting Huang Ting Huang Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain Frontiers in Microbiology Siniperca chuatsi Nocardia seriolae pathogenicity whole-genome analysis antibiotic susceptibility testing |
| title | Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain |
| title_full | Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain |
| title_fullStr | Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain |
| title_full_unstemmed | Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain |
| title_short | Pathogenicity and whole-genome analysis of a Siniperca chuatsi-derived Nocardia seriolae strain |
| title_sort | pathogenicity and whole genome analysis of a siniperca chuatsi derived nocardia seriolae strain |
| topic | Siniperca chuatsi Nocardia seriolae pathogenicity whole-genome analysis antibiotic susceptibility testing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1623741/full |
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