Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury
Abstract This study aimed to estimate the hepatic and immune ameliorating potential of extracted bovine lactoferrin (LF), Selenium nanoparticles (SeNPs) or their combination (LF/SeNPs) against bleomycin (BLM) induced hepatic injury. Fifty adult male rats (160–200 g) were equally divided into five gr...
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Nature Portfolio
2024-09-01
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| Online Access: | https://doi.org/10.1038/s41598-024-70894-6 |
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| author | Khaled G. Abdel-Wahhab Mahmoud Ashry Laila K. Hassan Marwa H. El-Azma Ghada M. Elqattan Mohamed H. A. Gadelmawla Fathia A. Mannaa |
| author_facet | Khaled G. Abdel-Wahhab Mahmoud Ashry Laila K. Hassan Marwa H. El-Azma Ghada M. Elqattan Mohamed H. A. Gadelmawla Fathia A. Mannaa |
| author_sort | Khaled G. Abdel-Wahhab |
| collection | DOAJ |
| description | Abstract This study aimed to estimate the hepatic and immune ameliorating potential of extracted bovine lactoferrin (LF), Selenium nanoparticles (SeNPs) or their combination (LF/SeNPs) against bleomycin (BLM) induced hepatic injury. Fifty adult male rats (160–200 g) were equally divided into five groups: (1) the saline control group, (2) BLM-injected (15 mg/kg twice a week, ip), and (3–5) groups treated orally with LF (200 mg/kg/day), SeNPs (0.0486 mg/kg/day) or LF/SeNPs combination (200.0486 mg/kg/day) for 6 weeks post BLM-intoxication. Blood and liver samples were subjected to biochemical, histopathological, and immunohistochemical analyses. The results revealed that BLM caused a significant increase in hepatic lipid peroxidation and nitric oxide, as well as serum markers of liver functions (AST, ALT and GGT activities), and levels of GM-CSF, CD4, TNF-α, IL-1β, TGF-β1, fibronectin, triglycerides, cholesterol and LDL-C. Additionally, hepatic glutathione, Na+/K+-ATPase, and glutathione peroxidase, as well as serum HDL-C, total protein and albumin levels were significantly reduced. Moreover, BLM injection resulted in marked histopathological alterations and severe expression of caspase 3. Post-treatment of BLM-intoxicated rats with LF, SeNPs or LF/SeNPs combination obviously improved the BLM-induced hepatic damages; this was achieved from the marked modulations in the mentioned parameters, besides improving the histopathological hepatic architecture. It is worth mentioning that LF/SeNPs exerted the greatest potency. In conclusion, the obtained results demonstrated that LF, SeNPs and LF/SeNPs succeeded in attenuating the BLM-induced hepatic dysfunction. Therefore, these supplements might be used to protect against drug-associated side effects. |
| format | Article |
| id | doaj-art-39810642707c4e5b9b0ea99efb319d97 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-39810642707c4e5b9b0ea99efb319d972025-02-02T12:25:13ZengNature PortfolioScientific Reports2045-23222024-09-0114111210.1038/s41598-024-70894-6Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injuryKhaled G. Abdel-Wahhab0Mahmoud Ashry1Laila K. Hassan2Marwa H. El-Azma3Ghada M. Elqattan4Mohamed H. A. Gadelmawla5Fathia A. Mannaa6Medical Physiology Department, National Research CentreZoology Department, Faculty of Science, Al-Azhar UniversityDairy Department, National Research CentreMedical Physiology Department, National Research CentreMedical Physiology Department, National Research CentreHistology Department, Faculty of Dentistry, Sinai UniversityMedical Physiology Department, National Research CentreAbstract This study aimed to estimate the hepatic and immune ameliorating potential of extracted bovine lactoferrin (LF), Selenium nanoparticles (SeNPs) or their combination (LF/SeNPs) against bleomycin (BLM) induced hepatic injury. Fifty adult male rats (160–200 g) were equally divided into five groups: (1) the saline control group, (2) BLM-injected (15 mg/kg twice a week, ip), and (3–5) groups treated orally with LF (200 mg/kg/day), SeNPs (0.0486 mg/kg/day) or LF/SeNPs combination (200.0486 mg/kg/day) for 6 weeks post BLM-intoxication. Blood and liver samples were subjected to biochemical, histopathological, and immunohistochemical analyses. The results revealed that BLM caused a significant increase in hepatic lipid peroxidation and nitric oxide, as well as serum markers of liver functions (AST, ALT and GGT activities), and levels of GM-CSF, CD4, TNF-α, IL-1β, TGF-β1, fibronectin, triglycerides, cholesterol and LDL-C. Additionally, hepatic glutathione, Na+/K+-ATPase, and glutathione peroxidase, as well as serum HDL-C, total protein and albumin levels were significantly reduced. Moreover, BLM injection resulted in marked histopathological alterations and severe expression of caspase 3. Post-treatment of BLM-intoxicated rats with LF, SeNPs or LF/SeNPs combination obviously improved the BLM-induced hepatic damages; this was achieved from the marked modulations in the mentioned parameters, besides improving the histopathological hepatic architecture. It is worth mentioning that LF/SeNPs exerted the greatest potency. In conclusion, the obtained results demonstrated that LF, SeNPs and LF/SeNPs succeeded in attenuating the BLM-induced hepatic dysfunction. Therefore, these supplements might be used to protect against drug-associated side effects.https://doi.org/10.1038/s41598-024-70894-6Nano-SeleniumBleomycinLiverLactoferrinFibrosisRats |
| spellingShingle | Khaled G. Abdel-Wahhab Mahmoud Ashry Laila K. Hassan Marwa H. El-Azma Ghada M. Elqattan Mohamed H. A. Gadelmawla Fathia A. Mannaa Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury Scientific Reports Nano-Selenium Bleomycin Liver Lactoferrin Fibrosis Rats |
| title | Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury |
| title_full | Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury |
| title_fullStr | Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury |
| title_full_unstemmed | Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury |
| title_short | Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury |
| title_sort | hepatic and immune modulatory effectiveness of lactoferrin loaded selenium nanoparticles on bleomycin induced hepatic injury |
| topic | Nano-Selenium Bleomycin Liver Lactoferrin Fibrosis Rats |
| url | https://doi.org/10.1038/s41598-024-70894-6 |
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