Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.

Cancer cachexia is accompanied by muscle atrophy, sharing multiple common catabolic pathways with sarcopenia, including mitochondrial dysfunction. This study investigated gene expression from skeletal muscle tissues of older healthy adults, who are at risk of age-related sarcopenia, to identify pote...

Full description

Saved in:
Bibliographic Details
Main Authors: Konstantinos Prokopidis, Panagiotis Giannos, Oliver C Witard, Daniel Peckham, Theocharis Ispoglou
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0273766&type=printable
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849469999338487808
author Konstantinos Prokopidis
Panagiotis Giannos
Oliver C Witard
Daniel Peckham
Theocharis Ispoglou
author_facet Konstantinos Prokopidis
Panagiotis Giannos
Oliver C Witard
Daniel Peckham
Theocharis Ispoglou
author_sort Konstantinos Prokopidis
collection DOAJ
description Cancer cachexia is accompanied by muscle atrophy, sharing multiple common catabolic pathways with sarcopenia, including mitochondrial dysfunction. This study investigated gene expression from skeletal muscle tissues of older healthy adults, who are at risk of age-related sarcopenia, to identify potential gene biomarkers whose dysregulated expression and protein interference were involved in non-small cell lung cancer (NSCLC). Screening of the literature resulted in 14 microarray datasets (GSE25941, GSE28392, GSE28422, GSE47881, GSE47969, GSE59880 in musculoskeletal ageing; GSE118370, GSE33532, GSE19804, GSE18842, GSE27262, GSE19188, GSE31210, GSE40791 in NSCLC). Differentially expressed genes (DEGs) were used to construct protein-protein interaction networks and retrieve clustering gene modules. Overlapping module DEGs were ranked based on 11 topological algorithms and were correlated with prognosis, tissue expression, and tumour purity in NSCLC. The analysis revealed that the dysregulated expression of the mammalian mitochondrial ribosomal proteins, Mitochondrial Ribosomal Protein S26 (MRPS26), Mitochondrial Ribosomal Protein S17 (MRPS17), Mitochondrial Ribosomal Protein L18 (MRPL18) and Mitochondrial Ribosomal Protein L51 (MRPL51) were linked to reduced survival and tumour purity in NSCLC while tissue expression of the same genes followed an opposite direction in healthy older adults. These results support a potential link between the mitochondrial ribosomal microenvironment in ageing muscle and NSCLC. Further studies comparing changes in sarcopenia and NSCLC associated cachexia are warranted.
format Article
id doaj-art-395d6aa2828f4e9f9d48403e66876a55
institution Kabale University
issn 1932-6203
language English
publishDate 2022-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-395d6aa2828f4e9f9d48403e66876a552025-08-20T03:25:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179e027376610.1371/journal.pone.0273766Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.Konstantinos ProkopidisPanagiotis GiannosOliver C WitardDaniel PeckhamTheocharis IspoglouCancer cachexia is accompanied by muscle atrophy, sharing multiple common catabolic pathways with sarcopenia, including mitochondrial dysfunction. This study investigated gene expression from skeletal muscle tissues of older healthy adults, who are at risk of age-related sarcopenia, to identify potential gene biomarkers whose dysregulated expression and protein interference were involved in non-small cell lung cancer (NSCLC). Screening of the literature resulted in 14 microarray datasets (GSE25941, GSE28392, GSE28422, GSE47881, GSE47969, GSE59880 in musculoskeletal ageing; GSE118370, GSE33532, GSE19804, GSE18842, GSE27262, GSE19188, GSE31210, GSE40791 in NSCLC). Differentially expressed genes (DEGs) were used to construct protein-protein interaction networks and retrieve clustering gene modules. Overlapping module DEGs were ranked based on 11 topological algorithms and were correlated with prognosis, tissue expression, and tumour purity in NSCLC. The analysis revealed that the dysregulated expression of the mammalian mitochondrial ribosomal proteins, Mitochondrial Ribosomal Protein S26 (MRPS26), Mitochondrial Ribosomal Protein S17 (MRPS17), Mitochondrial Ribosomal Protein L18 (MRPL18) and Mitochondrial Ribosomal Protein L51 (MRPL51) were linked to reduced survival and tumour purity in NSCLC while tissue expression of the same genes followed an opposite direction in healthy older adults. These results support a potential link between the mitochondrial ribosomal microenvironment in ageing muscle and NSCLC. Further studies comparing changes in sarcopenia and NSCLC associated cachexia are warranted.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0273766&type=printable
spellingShingle Konstantinos Prokopidis
Panagiotis Giannos
Oliver C Witard
Daniel Peckham
Theocharis Ispoglou
Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.
PLoS ONE
title Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.
title_full Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.
title_fullStr Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.
title_full_unstemmed Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.
title_short Aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non-small cell lung cancer.
title_sort aberrant mitochondrial homeostasis at the crossroad of musculoskeletal ageing and non small cell lung cancer
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0273766&type=printable
work_keys_str_mv AT konstantinosprokopidis aberrantmitochondrialhomeostasisatthecrossroadofmusculoskeletalageingandnonsmallcelllungcancer
AT panagiotisgiannos aberrantmitochondrialhomeostasisatthecrossroadofmusculoskeletalageingandnonsmallcelllungcancer
AT olivercwitard aberrantmitochondrialhomeostasisatthecrossroadofmusculoskeletalageingandnonsmallcelllungcancer
AT danielpeckham aberrantmitochondrialhomeostasisatthecrossroadofmusculoskeletalageingandnonsmallcelllungcancer
AT theocharisispoglou aberrantmitochondrialhomeostasisatthecrossroadofmusculoskeletalageingandnonsmallcelllungcancer