Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures
Due to the restricted intrinsic capacity of resident chondrocytes to regenerate the lost cartilage postinjury, stem cell-based therapies have been proposed as a novel therapeutic approach for cartilage repair. Moreover, stem cell-based therapies using mesenchymal stem cells (MSCs) or induced pluripo...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/9079538 |
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| author | Melissa Lo Monaco Greet Merckx Jessica Ratajczak Pascal Gervois Petra Hilkens Peter Clegg Annelies Bronckaers Jean-Michel Vandeweerd Ivo Lambrichts |
| author_facet | Melissa Lo Monaco Greet Merckx Jessica Ratajczak Pascal Gervois Petra Hilkens Peter Clegg Annelies Bronckaers Jean-Michel Vandeweerd Ivo Lambrichts |
| author_sort | Melissa Lo Monaco |
| collection | DOAJ |
| description | Due to the restricted intrinsic capacity of resident chondrocytes to regenerate the lost cartilage postinjury, stem cell-based therapies have been proposed as a novel therapeutic approach for cartilage repair. Moreover, stem cell-based therapies using mesenchymal stem cells (MSCs) or induced pluripotent stem cells (iPSCs) have been used successfully in preclinical and clinical settings. Despite these promising reports, the exact mechanisms underlying stem cell-mediated cartilage repair remain uncertain. Stem cells can contribute to cartilage repair via chondrogenic differentiation, via immunomodulation, or by the production of paracrine factors and extracellular vesicles. But before novel cell-based therapies for cartilage repair can be introduced into the clinic, rigorous testing in preclinical animal models is required. Preclinical models used in regenerative cartilage studies include murine, lapine, caprine, ovine, porcine, canine, and equine models, each associated with its specific advantages and limitations. This review presents a summary of recent in vitro data and from in vivo preclinical studies justifying the use of MSCs and iPSCs in cartilage tissue engineering. Moreover, the advantages and disadvantages of utilizing small and large animals will be discussed, while also describing suitable outcome measures for evaluating cartilage repair. |
| format | Article |
| id | doaj-art-395cd0a677224b58a20fcca2320df131 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-395cd0a677224b58a20fcca2320df1312025-02-03T05:59:07ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/90795389079538Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome MeasuresMelissa Lo Monaco0Greet Merckx1Jessica Ratajczak2Pascal Gervois3Petra Hilkens4Peter Clegg5Annelies Bronckaers6Jean-Michel Vandeweerd7Ivo Lambrichts8Department of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDepartment of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDepartment of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDepartment of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDepartment of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDepartment of Musculoskeletal Biology, Faculty of Health and Life Sciences, University of Liverpool, Leahurst Campus, Neston CH64 7TE, UKDepartment of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDepartment of Veterinary Medicine, Integrated Veterinary Research Unit-Namur Research Institute for Life Science (IVRU-NARILIS), Faculty of Sciences, University of Namur, 5000 Namur, BelgiumDepartment of Morphology, Biomedical Research Institute, Faculty of Medicine and Life Sciences, Hasselt University, Campus Diepenbeek, 3590 Diepenbeek, BelgiumDue to the restricted intrinsic capacity of resident chondrocytes to regenerate the lost cartilage postinjury, stem cell-based therapies have been proposed as a novel therapeutic approach for cartilage repair. Moreover, stem cell-based therapies using mesenchymal stem cells (MSCs) or induced pluripotent stem cells (iPSCs) have been used successfully in preclinical and clinical settings. Despite these promising reports, the exact mechanisms underlying stem cell-mediated cartilage repair remain uncertain. Stem cells can contribute to cartilage repair via chondrogenic differentiation, via immunomodulation, or by the production of paracrine factors and extracellular vesicles. But before novel cell-based therapies for cartilage repair can be introduced into the clinic, rigorous testing in preclinical animal models is required. Preclinical models used in regenerative cartilage studies include murine, lapine, caprine, ovine, porcine, canine, and equine models, each associated with its specific advantages and limitations. This review presents a summary of recent in vitro data and from in vivo preclinical studies justifying the use of MSCs and iPSCs in cartilage tissue engineering. Moreover, the advantages and disadvantages of utilizing small and large animals will be discussed, while also describing suitable outcome measures for evaluating cartilage repair.http://dx.doi.org/10.1155/2018/9079538 |
| spellingShingle | Melissa Lo Monaco Greet Merckx Jessica Ratajczak Pascal Gervois Petra Hilkens Peter Clegg Annelies Bronckaers Jean-Michel Vandeweerd Ivo Lambrichts Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures Stem Cells International |
| title | Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures |
| title_full | Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures |
| title_fullStr | Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures |
| title_full_unstemmed | Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures |
| title_short | Stem Cells for Cartilage Repair: Preclinical Studies and Insights in Translational Animal Models and Outcome Measures |
| title_sort | stem cells for cartilage repair preclinical studies and insights in translational animal models and outcome measures |
| url | http://dx.doi.org/10.1155/2018/9079538 |
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