L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies
ABSTRACT Introduction L‐Quebrachitol (LQB), a naturally occurring bioactive compound, exhibits anti‐inflammatory, anti‐oxidant, anti‐cancer, and anti‐diabetic properties. However, its therapeutic potential in convulsant management remains largely unexplored. The objective of this study was to invest...
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Wiley
2025-07-01
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| Series: | Brain and Behavior |
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| Online Access: | https://doi.org/10.1002/brb3.70675 |
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| author | Asifa Asrafi Mohammad Aslam Ali G. Alkhathami Md. Sakib Hossain Imam Hossen Rakib Md. Sakib Al Hasan Feroz Khan Nun Md. Faisal Amin Muhammad Torequl Islam |
| author_facet | Asifa Asrafi Mohammad Aslam Ali G. Alkhathami Md. Sakib Hossain Imam Hossen Rakib Md. Sakib Al Hasan Feroz Khan Nun Md. Faisal Amin Muhammad Torequl Islam |
| author_sort | Asifa Asrafi |
| collection | DOAJ |
| description | ABSTRACT Introduction L‐Quebrachitol (LQB), a naturally occurring bioactive compound, exhibits anti‐inflammatory, anti‐oxidant, anti‐cancer, and anti‐diabetic properties. However, its therapeutic potential in convulsant management remains largely unexplored. The objective of this study was to investigate the anticonvulsant effects of LQB in an In Vivo model and to examine its molecular interactions via In Silico docking simulations. Methods In the In Vivo study, pentylenetetrazol (PTZ) was administered intraperitoneally (i.p.) at 80 mg/kg to induce convulsions, and the test animals were treated orally with three doses of LQB (1, 5, and 10 mg/kg), with carbamazepine (CBZ) at 80 mg/kg as a standard drug. Results The results indicated that LQB at all tested doses significantly (p < 0.05) prolonged seizure latency and decreased convulsion frequency, with the 10 mg/kg dose showing the most significant effects. Furthermore, the combination of LQB (10 mg/kg) and CBZ (80 mg/kg) resulted in a synergistic increase in anticonvulsant activity. In the In Silico study, molecular docking analysis revealed that both LQB and CBZ interacted with the voltage‐gated sodium channel (VGSC), a key receptor involved in convulsions, with LQB demonstrating a binding affinity (BA) of −5.4 kcal/mol, which was moderate compared to CBZ's BA. Conclusion LQB showed potential anti‐convulsant activity in PTZ‐induced convulsion animals, possibly through blocking sodium channel receptors. Further studies are needed to clarify its mechanisms and clinical potential in convulsion treatment. |
| format | Article |
| id | doaj-art-39442d3c5f554a549cd60568d6d2127b |
| institution | DOAJ |
| issn | 2162-3279 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj-art-39442d3c5f554a549cd60568d6d2127b2025-08-20T02:48:43ZengWileyBrain and Behavior2162-32792025-07-01157n/an/a10.1002/brb3.70675L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico StudiesAsifa Asrafi0Mohammad Aslam1Ali G. Alkhathami2Md. Sakib Hossain3Imam Hossen Rakib4Md. Sakib Al Hasan5Feroz Khan Nun6Md. Faisal Amin7Muhammad Torequl Islam8Department of Biochemistry and Molecular Biology Gopalganj Science and Technology University Gopalganj BangladeshDepartment of Biochemistry and Molecular Biology Gopalganj Science and Technology University Gopalganj BangladeshDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences King Khalid University Abha Saudi ArabiaDepartment of Pharmacy Gopalganj Science and Technology University Gopalganj BangladeshDepartment of Pharmacy Gopalganj Science and Technology University Gopalganj BangladeshDepartment of Pharmacy Gopalganj Science and Technology University Gopalganj BangladeshDepartment of Pharmacy Gopalganj Science and Technology University Gopalganj BangladeshSchool of Integrative Biological and Chemical Sciences The University of Texas Rio Grande Valley Texas USADepartment of Pharmacy Gopalganj Science and Technology University Gopalganj BangladeshABSTRACT Introduction L‐Quebrachitol (LQB), a naturally occurring bioactive compound, exhibits anti‐inflammatory, anti‐oxidant, anti‐cancer, and anti‐diabetic properties. However, its therapeutic potential in convulsant management remains largely unexplored. The objective of this study was to investigate the anticonvulsant effects of LQB in an In Vivo model and to examine its molecular interactions via In Silico docking simulations. Methods In the In Vivo study, pentylenetetrazol (PTZ) was administered intraperitoneally (i.p.) at 80 mg/kg to induce convulsions, and the test animals were treated orally with three doses of LQB (1, 5, and 10 mg/kg), with carbamazepine (CBZ) at 80 mg/kg as a standard drug. Results The results indicated that LQB at all tested doses significantly (p < 0.05) prolonged seizure latency and decreased convulsion frequency, with the 10 mg/kg dose showing the most significant effects. Furthermore, the combination of LQB (10 mg/kg) and CBZ (80 mg/kg) resulted in a synergistic increase in anticonvulsant activity. In the In Silico study, molecular docking analysis revealed that both LQB and CBZ interacted with the voltage‐gated sodium channel (VGSC), a key receptor involved in convulsions, with LQB demonstrating a binding affinity (BA) of −5.4 kcal/mol, which was moderate compared to CBZ's BA. Conclusion LQB showed potential anti‐convulsant activity in PTZ‐induced convulsion animals, possibly through blocking sodium channel receptors. Further studies are needed to clarify its mechanisms and clinical potential in convulsion treatment.https://doi.org/10.1002/brb3.70675convulsionL‐Quebrachitolmolecular docking studysodium channel |
| spellingShingle | Asifa Asrafi Mohammad Aslam Ali G. Alkhathami Md. Sakib Hossain Imam Hossen Rakib Md. Sakib Al Hasan Feroz Khan Nun Md. Faisal Amin Muhammad Torequl Islam L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies Brain and Behavior convulsion L‐Quebrachitol molecular docking study sodium channel |
| title | L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies |
| title_full | L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies |
| title_fullStr | L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies |
| title_full_unstemmed | L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies |
| title_short | L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies |
| title_sort | l quebrachitol modulates the anti convulsant effects of carbamazepine possibly through voltage gated sodium channel blocking mechanism in chicks in vivo and in silico studies |
| topic | convulsion L‐Quebrachitol molecular docking study sodium channel |
| url | https://doi.org/10.1002/brb3.70675 |
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