PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES
Previous studies of the morphological features of head and neck squamous cell carcinomas revealed a significant association of the presence of discrete tumor cells and a low level of inflammatory infiltrate with lymphogenous metastasis. The aim of the study was to evaluate the quantity of tumor cell...
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Russian Academy of Sciences, Tomsk National Research Medical Center
2020-01-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/1245 |
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| author | V. A. Bychkov L. N. Bondar Ju. A. Nebova I. V. Larionova O. V. Cheremisina V. M. Perelmuter |
| author_facet | V. A. Bychkov L. N. Bondar Ju. A. Nebova I. V. Larionova O. V. Cheremisina V. M. Perelmuter |
| author_sort | V. A. Bychkov |
| collection | DOAJ |
| description | Previous studies of the morphological features of head and neck squamous cell carcinomas revealed a significant association of the presence of discrete tumor cells and a low level of inflammatory infiltrate with lymphogenous metastasis. The aim of the study was to evaluate the quantity of tumor cells undergoing epithelial-mesenchymal transition (EMt) in tumor cell structures of different degrees of differentiation and to assess the prognostic model of lymphogenous metastasis based on the studied parameters. Material and methods. the main clinical tumor characteristics and morphological patterns of the tumor were assessed in 92 patients. the expressions of snail and Vimentin proteins were studied in 16 patients using confocal microscopy. Results. the expression of the EMt marker in cell structures of different degrees of differentiation was evaluated; the maximum number of snail- and Vimentin-positive cells (20 % each) was observed in discrete tumor cells. No difference in the proportion of cells undergoing epithelial-mesenchymal transition between patients with and without lymphogenous metastasis was found. thus, the prognostic model of lymphogenous metastasis included: the presence of single tumor cells and low level of inflammation. the sensitivity of the model was 54.5 %, and specificity was 89 %. |
| format | Article |
| id | doaj-art-393dfd014a604de99a0742253bdd6f94 |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2020-01-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-393dfd014a604de99a0742253bdd6f942025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682020-01-01186677410.21294/1814-4861-2019-18-6-67-74691PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASESV. A. Bychkov0L. N. Bondar1Ju. A. Nebova2I. V. Larionova3O. V. Cheremisina4V. M. Perelmuter5Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, National Research Tomsk State UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, National Research Tomsk State UniversityCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of SciencesPrevious studies of the morphological features of head and neck squamous cell carcinomas revealed a significant association of the presence of discrete tumor cells and a low level of inflammatory infiltrate with lymphogenous metastasis. The aim of the study was to evaluate the quantity of tumor cells undergoing epithelial-mesenchymal transition (EMt) in tumor cell structures of different degrees of differentiation and to assess the prognostic model of lymphogenous metastasis based on the studied parameters. Material and methods. the main clinical tumor characteristics and morphological patterns of the tumor were assessed in 92 patients. the expressions of snail and Vimentin proteins were studied in 16 patients using confocal microscopy. Results. the expression of the EMt marker in cell structures of different degrees of differentiation was evaluated; the maximum number of snail- and Vimentin-positive cells (20 % each) was observed in discrete tumor cells. No difference in the proportion of cells undergoing epithelial-mesenchymal transition between patients with and without lymphogenous metastasis was found. thus, the prognostic model of lymphogenous metastasis included: the presence of single tumor cells and low level of inflammation. the sensitivity of the model was 54.5 %, and specificity was 89 %.https://www.siboncoj.ru/jour/article/view/1245epithelial-mesenchymal transitionintratumoral heterogeneityinflammationlymphogenous metastasis |
| spellingShingle | V. A. Bychkov L. N. Bondar Ju. A. Nebova I. V. Larionova O. V. Cheremisina V. M. Perelmuter PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES Сибирский онкологический журнал epithelial-mesenchymal transition intratumoral heterogeneity inflammation lymphogenous metastasis |
| title | PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES |
| title_full | PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES |
| title_fullStr | PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES |
| title_full_unstemmed | PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES |
| title_short | PROGNOSTIC SIGNIFICANCE OF THE EPITHELIAL-MESENCHYMAL TRANSITION MARKERS AND MORPHOLOGICAL CHARACTERISTICS OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS IN THE DEVELOPMENT OF LYMPHOGENOUS METASTASES |
| title_sort | prognostic significance of the epithelial mesenchymal transition markers and morphological characteristics of head and neck squamous cell carcinomas in the development of lymphogenous metastases |
| topic | epithelial-mesenchymal transition intratumoral heterogeneity inflammation lymphogenous metastasis |
| url | https://www.siboncoj.ru/jour/article/view/1245 |
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