The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds
The hard-seed coat of <i>Ormosia henryi</i> significantly impedes germination efficiency in massive propagation, while conventional physical dormancy-breaking methods often result in compromised seed vigor, asynchronous seedling emergence, and diminished stress tolerance. Seed biopriming...
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2025-07-01
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| author | Meng Ge Xiaoli Wei Yongming Fan Yan Wu Mei Fan Xueqing Tian |
| author_facet | Meng Ge Xiaoli Wei Yongming Fan Yan Wu Mei Fan Xueqing Tian |
| author_sort | Meng Ge |
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| description | The hard-seed coat of <i>Ormosia henryi</i> significantly impedes germination efficiency in massive propagation, while conventional physical dormancy-breaking methods often result in compromised seed vigor, asynchronous seedling emergence, and diminished stress tolerance. Seed biopriming, an innovative technique involving the inoculation of beneficial microorganisms onto seed surfaces or into germination substrates, enhances germination kinetics and emergence uniformity through microbial metabolic functions and synergistic interactions with seed exudates. Notably, spermosphere-derived functional bacteria isolated from native spermosphere soil demonstrate superior colonization capacity and sustained bioactivity. This investigation employed selective inoculation of these indigenous functional strains to systematically analyze dynamic changes in endogenous phytohormones, enzymatic activities, and storage substances during critical germination phases, thereby elucidating the physiological mechanisms underlying biopriming-enhanced germination. The experimental results demonstrated significant improvements in germination parameters through biopriming. Inoculation with the <i>Bacillus</i> sp. strain achieved a peak germination rate (76.19%), representing a 16.19% increase over the control (<i>p</i> < 0.05). The biopriming treatment effectively improved the seed vigor, broke the impermeability of the seed coat, accelerated the germination speed, and positively regulated physiological indicators, especially amylase activity and the ratio of gibberellic acid to abscisic acid. This study establishes a theoretical framework for microbial chemotaxis and rhizocompetence in seed priming applications while providing an eco-technological solution for overcoming germination constraints in <i>O. henryi</i> cultivation. The optimized biopriming protocol addresses both low germination rates and post-germination growth limitations, providing technical support for the seedling cultivation of <i>O. henryi</i>. |
| format | Article |
| id | doaj-art-391eb5ca8ed6403aab8fd8db7ce8dad5 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
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| series | Microorganisms |
| spelling | doaj-art-391eb5ca8ed6403aab8fd8db7ce8dad52025-08-20T03:35:38ZengMDPI AGMicroorganisms2076-26072025-07-01137159810.3390/microorganisms13071598The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> SeedsMeng Ge0Xiaoli Wei1Yongming Fan2Yan Wu3Mei Fan4Xueqing Tian5School of Human Settlements, North China University of Water Resources and Electric Power, Zhengzhou 450046, ChinaCollege of Forestry, Guizhou University, Guiyang 550025, ChinaSchool of Human Settlements, North China University of Water Resources and Electric Power, Zhengzhou 450046, ChinaSchool of Human Settlements, North China University of Water Resources and Electric Power, Zhengzhou 450046, ChinaCollege of Forestry, Guizhou University, Guiyang 550025, ChinaCollege of Forestry, Guizhou University, Guiyang 550025, ChinaThe hard-seed coat of <i>Ormosia henryi</i> significantly impedes germination efficiency in massive propagation, while conventional physical dormancy-breaking methods often result in compromised seed vigor, asynchronous seedling emergence, and diminished stress tolerance. Seed biopriming, an innovative technique involving the inoculation of beneficial microorganisms onto seed surfaces or into germination substrates, enhances germination kinetics and emergence uniformity through microbial metabolic functions and synergistic interactions with seed exudates. Notably, spermosphere-derived functional bacteria isolated from native spermosphere soil demonstrate superior colonization capacity and sustained bioactivity. This investigation employed selective inoculation of these indigenous functional strains to systematically analyze dynamic changes in endogenous phytohormones, enzymatic activities, and storage substances during critical germination phases, thereby elucidating the physiological mechanisms underlying biopriming-enhanced germination. The experimental results demonstrated significant improvements in germination parameters through biopriming. Inoculation with the <i>Bacillus</i> sp. strain achieved a peak germination rate (76.19%), representing a 16.19% increase over the control (<i>p</i> < 0.05). The biopriming treatment effectively improved the seed vigor, broke the impermeability of the seed coat, accelerated the germination speed, and positively regulated physiological indicators, especially amylase activity and the ratio of gibberellic acid to abscisic acid. This study establishes a theoretical framework for microbial chemotaxis and rhizocompetence in seed priming applications while providing an eco-technological solution for overcoming germination constraints in <i>O. henryi</i> cultivation. The optimized biopriming protocol addresses both low germination rates and post-germination growth limitations, providing technical support for the seedling cultivation of <i>O. henryi</i>.https://www.mdpi.com/2076-2607/13/7/1598bioprimingfunctional strainsgerminationmechanismphysiological indicatorsseed coat |
| spellingShingle | Meng Ge Xiaoli Wei Yongming Fan Yan Wu Mei Fan Xueqing Tian The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds Microorganisms biopriming functional strains germination mechanism physiological indicators seed coat |
| title | The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds |
| title_full | The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds |
| title_fullStr | The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds |
| title_full_unstemmed | The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds |
| title_short | The Physiological and Biochemical Mechanisms Bioprimed by Spermosphere Microorganisms on <i>Ormosia henryi</i> Seeds |
| title_sort | physiological and biochemical mechanisms bioprimed by spermosphere microorganisms on i ormosia henryi i seeds |
| topic | biopriming functional strains germination mechanism physiological indicators seed coat |
| url | https://www.mdpi.com/2076-2607/13/7/1598 |
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